Impaired Guanosine 3',5'-Cyclic Phosphate Production in Severe Pregnancy-Induced Hypertension with High Plasma Levels of Atrial and Brain Natriuretic Peptides.

Itoh, Hiroaki; Sagawa, Norimasa; Nanno, Hidetaka; Mori, Takahide; Mukoyama, Masashi; Itoh, Hiroshi; Nakao, Kazuwa

doi:10.1507/endocrj.44.389

“…One possible mechanism by which putative mutations in the ANP gene could exert their genetic effects is in determining presetting of ANP levels according to genotype, although plasma ANP levels are highly changeable within individuals to preserve sodium-water balance in the body. A few studies have reported distributions of plasma ANP levels in normal subjects (n l 10-36) compared with those in patients with essential hypertension [48], congestive heart failure [34] or pregnancy-induced hypertension [35]. The reported mean values in normotensive subjects (2n8-6n3 pmol\l) were not much different from our results, while concentrations were increased in diseased individuals to compensate for elevated intravascular volume overload and blood pressure.…”

Section: Discussioncontrasting

confidence: 94%

Genetic analysis of the atrial natriuretic peptide gene in essential hypertension

KATO¹,

SUGIYAMA²,

Morita³

et al. 2000

View full text Add to dashboard Cite

Atrial natriuretic peptide (ANP) plays an important role in the regulation of blood pressure through sodium-water homoeostasis. Accordingly, several investigators have raised the question of whether the gene encoding ANP is involved in the aetiology of essential hypertension or related phenotypes such as salt sensitivity. Most of the studies have used anonymous polymorphic markers of the gene, and made inconclusive claims about the disease relevance of ANP. Therefore, in order to find sequence variations with potential functional significance and to characterize the pattern of linkage disequilibrium between polymorphisms, we screened a 3368-bp genomic fragment of ANP. Subsequently we tested the association of detected polymorphisms with plasma ANP levels and with hypertension status. Two new polymorphisms were identified, in the 5'-untranslated region and exon 1 respectively, as well as three previously reported polymorphisms in intron 2 and exon 3. When analysed in 102 healthy normotensive subjects, none of the polymorphisms appeared to significantly affect plasma ANP levels. A case-control study in a Japanese population (255 hypertensive and 225 normotensive individuals) revealed a marginally significant association (P=0.026) between an ANP polymorphism located in the 5'-untranslated region (C-664G) and hypertension, but no association for the other polymorphisms. Each of the uncommon variants has an allele frequency of less than 10% in Japanese people, which may have hampered our detection of a significant association between ANP variants and hypertension status (and plasma ANP levels). The pathophysiological relevance of ANP, however, needs to be further defined in relation to hypertension-associated phenotypes, and also should be examined in different ethnic groups.

show abstract

“…Definition of normal values is needed for the use of NT‐proBNP during pregnancy. Previous studies aiming this issue had small cohorts and did not follow the subjects longitudinally (8–11). Furthermore, to our best knowledge, this is the first study comparing non‐pregnant women to pregnant women directly.…”

Section: Discussionmentioning

confidence: 99%

NT‐proBNP is increased in healthy pregnancies compared to non‐pregnant controls

Franz

¹

,

Andreas

²

,

Schießl

³

et al. 2009

Acta Obstet Gynecol Scand

View full text Add to dashboard Cite

Serum concentrations of the amino-terminal pro-B-type natriuretic peptide (NT-proBNP) may be used to monitor cardiac function during pregnancy but normal values are not established for this purpose. Therefore, we investigated NT-proBNP in normotensive healthy pregnancies compared to a non-pregnant control group. Serum NT-proBNP was measured in 94 normotensive, healthy pregnant women (32+/-6 years) every five weeks beginning from 12th gestational week (GW) in a longitudinal study and compared to a non-pregnant control group of 521 women (32+/-7 years). Pooled median serum NT-proBNP levels (25th; 75th percentile) were significantly higher in pregnant women compared to non-pregnant women (56 (33; 95) pg/ml vs. 38 (22; 62) pg/ml (p<0.001)). NT-proBNP increased during pregnancy to 73 (51; 124) pg/ml in the 11+6 to 15+6 GW (p<0.001). However, NT-proBNP levels from 23+0 GW towards term were comparable to non-pregnant controls. NT-proBNP is significantly elevated in healthy pregnancies until mid-pregnancy. As preeclampsia and gestational hypertension are associated with increased NT-proBNP, our results have to be considered in future diagnostic approaches using NT-proBNP for these pathologic conditions.

show abstract

“…Although the relevance of the amino acid substitution located in the prosegment of ANP to mature peptide function remains to be elucidated, a possible effect is indicated by the observation that a Gly-to-Ser substitution at position 99 (in exon 2) of the prosegment of rat ANP appears to be related to differential post-translational processing in two inbred rat strains, the stroke-prone spontaneously hypertensive rat and the Wistar Kyoto rat [47]. A few studies have reported distributions of plasma ANP levels in normal subjects (n l 10-36) compared with those in patients with essential hypertension [48], congestive heart failure [34] or pregnancy-induced hypertension [35]. This polymorphism is located within what would normally be a stop codon.…”

Section: Discussionmentioning

confidence: 99%

“…Measurement procedures have been described elsewhere [34,35]. Briefly, peripheral blood was taken after an overnight fast.…”

Section: Measurement Of Plasma Anp Levelsmentioning

confidence: 99%

Genetic analysis of the atrial natriuretic peptide gene in essential hypertension

Kato

¹

,

Sugiyama

²

,

Morita

³

et al. 2000

View full text Add to dashboard Cite

Atrial natriuretic peptide (ANP) plays an important role in the regulation of blood pressure through sodium-water homoeostasis. Accordingly, several investigators have raised the question of whether the gene encoding ANP is involved in the aetiology of essential hypertension or related phenotypes such as salt sensitivity. Most of the studies have used anonymous polymorphic markers of the gene, and made inconclusive claims about the disease relevance of ANP. Therefore, in order to find sequence variations with potential functional significance and to characterize the pattern of linkage disequilibrium between polymorphisms, we screened a 3368-bp genomic fragment of ANP. Subsequently we tested the association of detected polymorphisms with plasma ANP levels and with hypertension status. Two new polymorphisms were identified, in the 5'-untranslated region and exon 1 respectively, as well as three previously reported polymorphisms in intron 2 and exon 3. When analysed in 102 healthy normotensive subjects, none of the polymorphisms appeared to significantly affect plasma ANP levels. A case-control study in a Japanese population (255 hypertensive and 225 normotensive individuals) revealed a marginally significant association (P=0.026) between an ANP polymorphism located in the 5'-untranslated region (C-664G) and hypertension, but no association for the other polymorphisms. Each of the uncommon variants has an allele frequency of less than 10% in Japanese people, which may have hampered our detection of a significant association between ANP variants and hypertension status (and plasma ANP levels). The pathophysiological relevance of ANP, however, needs to be further defined in relation to hypertension-associated phenotypes, and also should be examined in different ethnic groups.

show abstract

Impaired Guanosine 3',5'-Cyclic Phosphate Production in Severe Pregnancy-Induced Hypertension with High Plasma Levels of Atrial and Brain Natriuretic Peptides.

Abstract: Abstract.To But in the pregnant women with severe PIH, plasma cGMP levels were significantly lower than those in pregnant women with mild PIH (P<0.05), although plasma ANP and BNP levels were higher than those in pregnant women with mild PIH.

Cited by 20 publications

References 13 publications

Genetic analysis of the atrial natriuretic peptide gene in essential hypertension

Genetic analysis of the atrial natriuretic peptide gene in essential hypertension

NT‐proBNP is increased in healthy pregnancies compared to non‐pregnant controls

Genetic analysis of the atrial natriuretic peptide gene in essential hypertension

Contact Info

Product

Resources

About