Impaired growth and differentiation of diploid but not immortal melanoblasts from endothelin receptor B mutant (piebald) mice

Sviderskaya, Elena V.; Easty, David J.; Bennett, Dorothy C.

doi:10.1002/(sici)1097-0177(199812)213:4<452::aid-aja10>3.0.co;2-6

Cited by 11 publications

(3 citation statements)

References 47 publications

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“…Endothelins can signal through two receptors, endothelin receptors A and B (Ednra, Ednrb). Some response to these might have been expected from the genetic (Baynash et al, 1994; Shin et al, 1999) and biological (Ono et al, 1998; Opdecamp et al, 1998) evidence that Edn3 and Ednrb are required for normal melanoblast and melanocyte development, but the observed lack of response is consistent with the lack of detectable Ednrb mRNA in mouse melanoblast lines including melb‐a (Sviderskaya et al, 1998). Work reported in an abstract suggests that melanocyte precursors cultured extensively with and without Edn3, respectively, did and did not detectably express immunoreactive Ednrb (Kawa et al, 1999).…”

Section: Discussionmentioning

confidence: 66%

“…We, therefore, developed methods to immortalize melanoblasts, and have described 6 lines of these cells, all from mice of a nonagouti ( a/a , black) genetic background. The melb‐a line used in the present study is a/a and otherwise wild‐type (Sviderskaya et al, 1995); others are from pinkeyed dilution , piebald (endothelin receptor B mutant, Ednrb s /Ednrb s ) (3 lines) and misty ( m/m ) mice (Sviderskaya et al, 1997, 1998; reviewed by Bennett and Sviderskaya, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Agouti signaling protein and other factors modulating differentiation and proliferation of immortal melanoblasts

Sviderskaya

Hill

Balachandar

et al. 2001

Developmental Dynamics

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Agouti signaling protein and other factors modulating differentiation and proliferation of immortal melanoblasts

Sviderskaya

Hill

Balachandar

et al. 2001

Developmental Dynamics

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“…Therefore, to precisely assess the effects of extrinsic factors, it is preferable to use cell lines of melanocyte precursors which can be maintained without feeder cells. Immortal melanoblasts or melanocyte cell lines without feeder cells (10–12) derived from mouse skin have been established. However, cell lines without feeder cells from mouse neural crest cells have not yet been established.…”

Section: Introductionmentioning

confidence: 99%

Establishment by an Original Single‐cell Cloning Method and Characterization of an Immortal Mouse Melanoblast Cell Line (NCCmelb4)

Ito

Kawa

Watabe

et al. 2004

Pigment Cell Research

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We devised a unique new single-cell cloning method which uses microscope cover glasses and established a melanoblast cell line derived from mouse neural crest cells. A microscope cover glass was nicked and broken into small pieces and put on a dish. Culture medium and a suspension of 20-30 cells/ml were dropped in the dish. After 1-3 d, a piece of glass to which only one cell was adhered was picked up and transferred to another dish containing culture medium. The greatest advantage of this method is that the derivation of a colony from a single cell can be directly confirmed by microscopy and there is no risk of migratory cells being contaminated by other colonies. Using this single-cell cloning method, in this study we established a cell line derived from a neural crest cell line (NCC-S4.1) and designated it as NCCmelb4. When the culture medium was supplemented with stem cell factor (SCF) alone, NCCmelb4 cells were KIT-positive and tyrosinase-negative melanocyte precursors; they remained at an immature and undifferentiated stage. When the medium was supplemented with phorbol 12-o-tetradecanoyl-13-acetate (TPA) + cholera toxin (CT), the cell morphology changed and became L-3,4-dihydroxyphenylalanine (DOPA)-positive. This observation indicates that the NCCmelb4 cells are capable of further differentiation with suitable stimulation. NCCmelb4 cells derived from the mouse neural crest has characteristics of melanocyte precursors (melanoblasts), and is a cell line which can be utilized to study differentiation-inducing factors and growth factors without the effects of feeder cells.

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Mutational Analysis of Endothelin Receptor b1 (rose) during Neural Crest and Pigment Pattern Development in the Zebrafish Danio rerio

Parichy

Mellgren

Rawls

et al. 2000

Developmental Biology

220

232

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Pigment patterns of fishes are a tractable system for studying the genetic and cellular bases for postembryonic phenotypes. In the zebrafish Danio rerio, neural crest-derived pigment cells generate different pigment patterns during different phases of the life cycle. Whereas early larvae exhibit simple stripes of melanocytes and silver iridophores in a background of yellow xanthophores, this pigment pattern is transformed at metamorphosis into that of the adult, comprising a series of dark melanocyte and iridophore stripes, alternating with light stripes of iridophores and xanthophores. Although several genes have been identified in D. rerio that contribute to the development of both early larval and adult pigment patterns, comparatively little is known about genes that are essential for pattern formation during just one or the other life cycle phase. In this study, we identify the gene responsible for the rose mutant phenotype in D. rerio. rose mutants have wild-type early larval pigment patterns, but fail to develop normal numbers of melanocytes and iridophores during pigment pattern metamorphosis and exhibit a disrupted pattern of these cells. We show that rose corresponds to endothelin receptor b1 (ednrb1), an orthologue of amniote Ednrb genes that have long been studied for their roles in neural crest and pigment cell development. Furthermore, we demonstrate that D. rerio ednrb1 is expressed both during pigment pattern metamorphosis and during embryogenesis, and cells of melanocyte, iridophore, and xanthophore lineages all express this gene. These analyses suggest a phylogenetic conservation of roles for Ednrb signaling in the development of amniote and teleost pigment cell precursors. As murine Ednrb is essential for the development of all neural crest derived melanocytes, and D. rerio ednrb1 is required only by a subset of adult melanocytes and iridophores, these analyses also reveal variation among vertebrates in the cellular requirements for Ednrb signaling, and suggest alternative models for the cellular and genetic bases of pigment pattern metamorphosis in D. rerio.

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Impaired growth and differentiation of diploid but not immortal melanoblasts from endothelin receptor B mutant (piebald) mice

Cited by 11 publications

References 47 publications

Agouti signaling protein and other factors modulating differentiation and proliferation of immortal melanoblasts

Agouti signaling protein and other factors modulating differentiation and proliferation of immortal melanoblasts

Establishment by an Original Single‐cell Cloning Method and Characterization of an Immortal Mouse Melanoblast Cell Line (NCCmelb4)

Mutational Analysis of Endothelin Receptor b1 (rose) during Neural Crest and Pigment Pattern Development in the Zebrafish Danio rerio

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