Impaired gonadal and somatic development corroborate vulnerability differences to the synthetic estrogen ethinylestradiol among deeply diverged anuran lineages

Tamschick, Stephanie; Rozenblut-Kościsty, Beata; Ogielska, Maria; Lehmann, Andreas; Lymberakis, Petros; Hoffmann, Frauke; Lutz, Ilka; Schneider, R.; Kloas, Werner; Stöck, Matthias

doi:10.1016/j.aquatox.2016.07.001

Cited by 11 publications

(13 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…60% under higher Tb-concentrations (0.27 and 2.7 μg/L). Despite (naturally except for the treatment) identical rearing conditions like in our previous experiments involving EE2 and BPA 30–32 , in the present trials we observed increased mortality in green toads. However, this mortality in B .…”

Section: Discussionsupporting

confidence: 59%

“…We used the same high standard flow-through system as in our former experiments involving EE2 30,32 and BPA 31 . During the Tb-exposure, we experienced some fluctuations in the maintenance of the nominal Tb-concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Among others, this has been shown for feminizing substances, such as 17α or β-ethinylestradiol and BPA. Examples come from Xenopus laevis 27–32 , Bombina bombina and Bombina variegata 29 , Hyla arborea 29–32 , Bufo(tes) viridis 29–32 , Lithobates sylvaticus 33 , Euphlyctis cyanophlyctis 34 , and Rana curtipes 35 . On the other hand, vulnerability to masculinizing substances has been tested using testosterone, for example in Rana rugosa 36 , Rana japonica 37 , Pelophylax nigromaculatus 34 , and Rana curtipes 35 , and often resulted in sex reversals.…”

Section: Introductionmentioning

confidence: 99%

“…Some of us have recently developed a novel methodology for EDC testing in amphibians, comprising synchronized tadpole exposure of representatives of three systematic anuran families and pioneering genetic and histological sexing of metamorphs in a flow-through-system 30 , with which we have, so far, tested two feminizing substances: EE2 and BPA 30–32 . This approach allowed for the first time the immediate comparison of EDC effects on the sexual development of three deeply divergent model and non-model anurans under identical experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Impacts of the synthetic androgen Trenbolone on gonad differentiation and development – comparisons between three deeply diverged anuran families

Rozenblut-Kościsty¹,

Ogielska²,

Hahn

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Using a recently developed approach for testing endocrine disruptive chemicals (EDCs) in amphibians, comprising synchronized tadpole exposure plus genetic and histological sexing of metamorphs in a flow-through-system, we tested the effects of 17β-Trenbolone (Tb), a widely used growth promoter in cattle farming, in three deeply diverged anuran families: the amphibian model species Xenopus laevis (Pipidae) and the non-models Bufo ( tes ) viridis (Bufonidae) and Hyla arborea (Hylidae). Trenbolone was applied in three environmentally and/or physiologically relevant concentrations (0.027 µg/L (10 −10 M), 0.27 µg/L (10 −9 M), 2.7 µg/L (10 −8 M)). In none of the species, Tb caused sex reversals or masculinization of gonads but had negative species-specific impacts on gonad morphology and differentiation after the completion of metamorphosis, independently of genetic sex. In H . arborea and B . viridis , mounting Tb-concentration correlated positively with anatomical abnormalities at 27 µg/L (10 −9 M) and 2.7 µg/L (10 −8 M), occurring in X . laevis only at the highest Tb concentration. Despite anatomical aberrations, histologically all gonadal tissues differentiated seemingly normally when examined at the histological level but at various rates. Tb-concentration caused various species-specific mortalities (low in Xenopus , uncertain in Bufo ). Our data suggest that deep phylogenetic divergence modifies EDC-vulnerability, as previously demonstrated for Bisphenol A (BPA) and Ethinylestradiol (EE2).

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impacts of the synthetic androgen Trenbolone on gonad differentiation and development – comparisons between three deeply diverged anuran families

Rozenblut-Kościsty¹,

Ogielska²,

Hahn

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…BPA is widely found in aquatic environments and causes reproductive toxicity in fish, often resulting in abnormal testicular and ovarian development [16][17][18]28,29]. BPA has also been demonstrated to accumulate in various fish tissues, leading to pathological changes, including changes in the endocannabinoid system, changes in the ontogeny of the cortisol stress response, and energetic impairments [22,23,30].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Effects of Bisphenol A on Dopamine Synthesis and Blood Vessels in the Goldfish Brain

Wang

Lin

et al. 2019

IJMS

View full text Add to dashboard Cite

Bisphenol A (BPA) is an abundant contaminant found in aquatic environments. While a large number of toxicological studies have investigated the effects of BPA, the potential effects of BPA exposure on fish brain have rarely been studied. To understand how BPA impacts goldfish brains, we performed a transcriptome analysis of goldfish brains that had been exposed to 50 μg L−1 and 0 μg L−1 BPA for 30 days. In the analysis of unigene expression profiles, 327 unigenes were found to be upregulated and 153 unigenes were found to be downregulated in the BPA exposure group compared to the control group. Dopaminergic signaling pathway-related genes were significantly downregulated in the BPA exposure group. Furthermore, we found that serum dopamine concentrations decreased and TUNEL (terminal deoxynucleotidyl transferase 2-deoxyuridine, 5-triphosphate nick end labeling) staining was present in dopamine neurons enriched regions in the brain after BPA exposure, suggesting that BPA may disrupt dopaminergic processes. A KEGG analysis revealed that genes involved in the fluid shear stress and atherosclerosis pathway were highly significantly enriched. In addition, the qRT-PCR results for fluid shear stress and atherosclerosis pathway-related genes and the vascular histology of the brain showed that BPA exposure could damage blood vessels and induce brain atherosclerosis. The results of this work provide insights into the biological effects of BPA on dopamine synthesis and blood vessels in goldfish brain and could lay a foundation for future BPA neurotoxicity studies.

show abstract

Effect of ethylenethiourea on metamorphosis and ovary development: A comparative study of three larval frogs

2021

View full text Add to dashboard Cite

Amphibian endocrine systems interact with each other during normal development.Interference with one of the endocrine systems may influence others. We studied the effect of a thyroid inhibitor (ethylenethiourea [ETU]) on metamorphosis and ovary development of three species, Sphaerotheca pashchima, Indosylvirana caesari, and Euphlyctis cyanophlyctis with different larval durations. We treated the tadpoles of these species with 50, 100, and 200 mg/L concentrations of ETU and studied their larval duration, size at metamorphosis, and ovary development. The results revealed that ETU affects metamorphosis, depending on the species and concentration. ETU delayed metamorphosis of E. cyanophlyctis tadpoles and did not affect metamorphosis in S. pashchima tadpoles. Lower concentrations of ETU stimulated metamorphosis in I. caesari tadpoles while high concentration delayed metamorphosis. In the tadpoles (E. cyanophlyctis) treated with higher concentrations of ETU, ovary development was advanced with an increased size of the diplotene oocytes. Oocyte size was smaller in the tadpoles (of I. caesari) treated with lower concentrations of ETU. These results demonstrated that the tadpoles of these species show different responses to the thyroid inhibitor, possibly due to the differences in the larval duration and sensitivity. Inhibition or acceleration of metamorphosis did not interfere in the ovary development of E. cyanophlyctis and I. caesari. These results will be useful in understanding the impact of endocrine disruptors on the interaction between thyroid and sex steroid hormones.

show abstract

Impaired gonadal and somatic development corroborate vulnerability differences to the synthetic estrogen ethinylestradiol among deeply diverged anuran lineages

Cited by 11 publications

References 72 publications

Impacts of the synthetic androgen Trenbolone on gonad differentiation and development – comparisons between three deeply diverged anuran families

Impacts of the synthetic androgen Trenbolone on gonad differentiation and development – comparisons between three deeply diverged anuran families

Assessment of the Effects of Bisphenol A on Dopamine Synthesis and Blood Vessels in the Goldfish Brain

Effect of ethylenethiourea on metamorphosis and ovary development: A comparative study of three larval frogs

Contact Info

Product

Resources

About