Impaired Gamma Interferon Response to
            <i>Mycobacterium vaccae</i>
            Antigens in Patients with Cavitary Pulmonary Tuberculosis

Rodríguez-Güell, Elisabeth; Agustí, Gemma; Corominas, M.; Luquin, Marina; Julián, Esther

doi:10.1128/cvi.00131-08

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Barry, et al [ 11 ] demonstrated impaired antigen-specific CD4+ T lymphocytes in cavitary tuberculosis, and other studies [ 26 , 27 ] also reported impaired antigen specific IFN-γ in cavitary tuberculosis, but no one made the stratification analysis for cavitary PTB by number and diameter of cavities, their numbers of participants enrolled were much lower than current study. At the site of infection, the proliferation reaction and immune response were also weaker in cavitary tuberculosis as reported by two studies [ 28 , 29 ].…”

Section: Discussioncontrasting

confidence: 56%

Impaired M. tuberculosis Antigen-Specific IFN-γ Response without IL-17 Enhancement in Patients with Severe Cavitary Pulmonary Tuberculosis

et al. 2015

View full text Add to dashboard Cite

BackgroundTh1 cells play an essential role in immune protection against tuberculosis. Th17 cells might be involved with immune pathology in active human tuberculosis (TB). The balance between Th1 and Th17 cells in patients with cavitary tuberculosis needs to be clarified which might help understanding the immunological basis of pathologic pathogenesis in TB.MethodInitially treated pulmonary TB (PTB) patients with or without cavities were recruited before chemotherapy. We isolated peripheral blood mononuclear cells, stimulated with phytohemagglutinin (PHA), PPD, or ESAT-6 antigens, and assayed supernatant IFN-γ and IL-17 by ELISA after 24 or 72 hours incubation, respectively. Cells were also stained with antibodies to CD3, CD4, CD8, IFN-γ or IL-17 and the proportion of stained cells was measured by flow cytometry.ResultsWe found wide variation of IFN-γ response in active PTB patients, but less subject-to-subject variation of IL-17 was observed as we previously reported. There were no significant differences in IFN-γ and IL-17 between cavitary and non-cavitary PTB; however, we found decreased IFN-γ secretion in severe cavitary PTB compared to mild lesion non-cavitary PTB (p < 0.05). We also found a decrease in the proportion of CD3+CD4+ T cells in the blood of severe cavitary PTB patients (p < 0.05).ConclusionsIL-17 seemed to have no association with the formation of cavities in active PTB from the study of PBMC. Impaired IFN-γ without IL-17 enhancement occurs in peripheral blood during severe cavitary PTB. Our results demonstrate that M. tuberculosis antigen-specific Th1 response is decreased when PTB lesions develop to severe cavities.

show abstract