Impaired counter-regulation of interleukin-1 by the soluble IL-1 receptor type II in patients with chronic liver disease

Ludwiczek, Othmar; Vannier, Edouard; Moschen, Alexander R.; Salazar-Montes, Adriana; Borggraefe, Ingo; Gabay, Cem; Enrich, Barbara; Kaser, Arthur; Siegmund, Britta; Dinarello, Charles A.; Tilg, Herbert

doi:10.1080/00365520802179925

Cited by 16 publications

(17 citation statements)

References 33 publications

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“…In this study, the plasma levels of IL‐1α, IL‐1β and IL‐1Ra were elevated in cirrhotic patients compared with healthy controls. This result is in agreement with the previous research . However, the mechanism is unclear.…”

Section: Discussionsupporting

confidence: 92%

“…Some surrogate markers have been proposed for bacterial translocation but they did not correlate with the severity of cirrhosis or development of bacterial infection . Previous studies have shown elevated plasma levels of IL‐1α, IL‐1β and IL‐1 receptor antagonist (IL‐1Ra) in patients with liver cirrhosis . The IL‐1 family of cytokines comprises 11 proteins with the main function of controlling pro‐inflammatory reaction in response to tissue injury .…”

Section: Introductionmentioning

confidence: 99%

“…8 Previous studies have shown elevated plasma levels of IL-1α, IL-1β and IL-1 receptor antagonist (IL-1Ra) in patients with liver cirrhosis. [9][10][11] The IL-1 family of cytokines comprises 11 proteins with the main function of controlling pro-inflammatory reaction in response to tissue injury. 12 Macrophages and monocytes are the major source of IL-1α and IL-1β.…”

Section: Introductionmentioning

confidence: 99%

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Interleukin‐1 receptor antagonist correlates with hepatic venous pressure gradient and predicts occurrence of overall complications and bacterial infections in patients with cirrhosis

Hsieh

Lee

Yang

et al. 2014

Hepatology Research

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interleukin‐1 receptor antagonist correlates with hepatic venous pressure gradient and predicts occurrence of overall complications and bacterial infections in patients with cirrhosis

Hsieh

Lee

Yang

et al. 2014

Hepatology Research

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“…Chondrocytes may lose the cortical actin distribution and develop cellular processes extending from the plasma membrane, becoming more fibroblast-like under certain culturing conditions (11,18). Interestingly, a similar morphological change has been associated in osteoarthritic chondrocytes (5).…”

Section: Resultsmentioning

confidence: 99%

Optimal 3D Culture of Primary Articular Chondrocytes for Use in the Rotating Wall Vessel Bioreactor

Mellor¹,

Baker²,

Brown³

et al. 2014

aviat space environ med

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INTRODUCTION Reliable culturing methods for primary articular chondrocytes are essential to study the effects of loading and unloading on joint tissue at the cellular level. Due to the limited proliferation capacity of primary chondrocytes and their tendency to dedifferentiate in conventional culture conditions, long-term culturing conditions of primary chondrocytes can be challenging. The goal of this study was to develop a suspension culturing technique that not only would retain the cellular morphology but also maintain gene expression characteristics of primary articular chondrocytes. METHODS Three-dimensional culturing methods were compared and optimized for primary articular chondrocytes in the rotating wall vessel bioreactor, which changes the mechanical culture conditions to provide a form of suspension culture optimized for low shear and turbulence. We performed gene expression analysis and morphological characterization of cells cultured in alginate beads, Cytopore-2 microcarriers, primary monolayer culture, and passaged monolayer cultures using reverse transcription-PCR and laser scanning confocal microscopy. RESULTS Primary chondrocytes grown on Cytopore-2 microcarriers maintained the phenotypical morphology and gene expression pattern observed in primary bovine articular chondrocytes, and retained these characteristics for up to 9 days. DISCUSSION Our results provide a novel and alternative culturing technique for primary chondrocytes suitable for studies that require suspension such as those using the rotating wall vessel bioreactor. In addition, we provide an alternative culturing technique for primary chondrocytes that can impact future mechanistic studies of osteoarthritis progression, treatments for cartilage damage and repair, and cartilage tissue engineering.

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“…At the molecular level NALPs promote the formation of large multi-protein signalling complexes called inflammasomes that activate inflammatory Caspases-1, -4 and -5 [92]. While inflammatory caspases are associated with apoptosis of infected macrophage apoptosis -called pyroptosis [93] these caspases are predominantly involved in processing inflammatory cytokines such as Interleukin-1 and -33 and promoting the inflammatory response [94]. Upon processing, interleukins -as extracellular signalling molecules -bind their cognate receptors and, in an autocrine or paracrine manner, activate the NF B pathway [95].…”

Section: Nalp1mentioning

confidence: 99%

A Prickly Subject: Apoptotic Regulation by Hedgehog Morphogens

Ditzel¹

2011

Open Cell Signal J

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Morphogens, as intercellular signalling proteins, provide a non-cell-autonomous mechanism to impart positional information to cells and govern essential cellular processes such as apoptosis. Individual morphogen pathways utilise diverse strategies to regulate the assembly and activity of the pro-apoptotic multi-protein complexes -namely the apoptosome and the death-inducing signalling complex (DISC). This review aims to highlight the apoptotic regulatory mechanisms utilised by the Hedgehog (HH) morphogen pathway -with particular emphasis on a novel Caspase-9 activating complex and the utilisation of a pro-apoptotic autocrine-signalling loop.

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Impaired counter-regulation of interleukin-1 by the soluble IL-1 receptor type II in patients with chronic liver disease

Cited by 16 publications

References 33 publications

Interleukin‐1 receptor antagonist correlates with hepatic venous pressure gradient and predicts occurrence of overall complications and bacterial infections in patients with cirrhosis

Interleukin‐1 receptor antagonist correlates with hepatic venous pressure gradient and predicts occurrence of overall complications and bacterial infections in patients with cirrhosis

Optimal 3D Culture of Primary Articular Chondrocytes for Use in the Rotating Wall Vessel Bioreactor

A Prickly Subject: Apoptotic Regulation by Hedgehog Morphogens

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