Impaired balance of type I and type III procollagen mRNA in cultured fibroblasts of patients with incisional hernia

Si, Zhongyi; Bhardwaj, R.; Rösch, R.; Merten, Peter Rene; Klosterhalfen, B.; Klinge, U.

doi:10.1067/msy.2002.121376

Cited by 107 publications

(61 citation statements)

References 27 publications

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“…In our study, 62 patients developed incisional hernia within a period of 1 year of previous surgery. Similar findings were noted by Ankman et al 14 Maximum patients (70) were presented with pain and swelling and other patients with irreducible swelling (20) and strangulation (10). This was in consistent with the findings of Bhamre et al in which pain and swelling was noted in 55.5% of patients.…”

Section: Discussionsupporting

confidence: 91%

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Prospective and retrospective study of incisional hernias in a tertiary care hospital

Harikrishnan

Vakayil

2017

Int Surg J

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Background: Incisional hernias are the most common complications of laparotomy. The aim of the present study was to assess and analyse the risk factors leading to development of incisional hernia, different modalities of treatment adopted, postoperative complications and surgical outcome in these patients on follow up. Methods: This was a combined prospective and retrospective study conducted on 100 cases of incisional hernia admitted to the Department of Surgery, Jubilee Mission Medical College Thrissur, Kerala during the period 2013 -2015. The technique of the repair was decided by the size of the hernial defect, abdominal muscle tone and general condition of the patient. Postoperatively patients were followed up for detection of possible complications and their treatment. Results: Majority of the patients were in the age group of 41-50 years. Female preponderance was observed. Postpartum sterilisation in about 30 cases tops the list of prior operation predisposing to incisional hernia. Pain and swelling was the most common complaint noted in 70 cases. The major risk factor noted was cough in about 60% cases. Elective surgeries were done in 85cases and emergency surgeries in 15 cases. Nausea was the immediate complication observed in about 25 cases. Pain and induration was the major late complication observed in 60 cases. Conclusions: Incisional hernias are more common among females. Cough following previous surgery was the most important risk factor followed by wound infection. Among the repair techniques, complications were lesser and recurrence least for those repaired by mesh, darning and transverse double breasting (Mayo's).

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Section: Discussionsupporting

confidence: 91%

“…Hence, tension free repairs are recommended. 10 Our present study was aimed to identify the risk factors associated with incisional hernia as well as factors affecting recurrence and to study the management and postoperative complications of incisional hernia.…”

Section: Introductionmentioning

confidence: 99%

Prospective and retrospective study of incisional hernias in a tertiary care hospital

Harikrishnan

Vakayil

2017

Int Surg J

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“…15,53,54 Previous work has established altered type I/III collagen ratios in IHFs. 55 Here, we report that fascia IHFs display reduced cell size, increased proliferation and migration rates, and decreased strength of adhesion, in parallel with an altered cytoskeleton network, fewer ␣-SMApositive stress fibers, and smaller focal contacts. 56,57 Moreover, they show enhanced sensitivity to proapoptotic challenges, as well as features compatible with compromised autophagic/lysosomal processes.…”

Section: Discussionmentioning

confidence: 67%

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Díaz

Quiles

Guillem-Marti

et al. 2011

The American Journal of Pathology

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Incisional hernia often occurs following laparotomy and can be a source of serious problems. Although there is evidence that a biological cause may underlie its development, the mechanistic link between the local tissue microenvironment and tissue rupture is lacking. In this study, we used matched tissue-based and in vitro primary cell culture systems to examine the possible involvement of fascia fibroblasts in incisional hernia pathogenesis. Fascia biopsies were collected at surgery from incisional hernia patients and non-incisional hernia controls. Tissue samples were analyzed by histology and immunoblotting methods. Fascia primary fibroblast cultures were assessed at morphological, ultrastructural, and functional levels. We document tissue and fibroblast loss coupled to caspase-3 activation and induction of apoptosis-like cell-death mechanisms in incisional hernia fascia. Alterations in cytoskeleton organization and solubility were also observed. Incisional hernia fibroblasts showed a consistent phenotype throughout early passages in vitro, which was characterized by significantly enhanced cell proliferation and migration, reduced adhesion, and altered cytoskeleton properties, as compared to non-incisional hernia fibroblasts. Moreover, incisional hernia fibroblasts displayed morphological and ultrastructural alterations compatible with autophagic processes or lysosomal dysfunction, together with enhanced sensitivity to proapoptotic challenges. Overall, these data suggest an ongoing complex interplay of cell death induction, aberrant fibroblast function, and tissue loss in incisional hernia fascia, which may significantly contribute to altered matrix maintenance and tissue rupture in vivo.

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“…iNOS, inducible nitric oxide synthase. type 1 and type 3 collagen fibers coexist within individual fibrils, their relative ratios play an important role in the regulation of fibrillogenesis and in the determination of the final fibular diameter and bundle architecture [32]. The mature type 1 collagen is responsible for mechanical stability, whereas the type 3 collagen that forms thin fibers can be regarded as immature collagen of the early wound healing process.…”

Section: Figmentioning

confidence: 99%

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

Linard

Tissedre

Busson

et al. 2015

Stem Cells and Development

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Mesenchymal stem cell (MSC) therapy has recently been investigated as a potential treatment for cutaneous radiation burns. We tested the hypothesis that injection of local gingival fibroblasts (GFs) would promote healing of radiation burn lesions and compared results with those for MSC transplantation. Human clinicalgrade GFs or bone marrow-derived MSCs were intradermally injected into mice 21 days after local leg irradiation. Immunostaining and real-time PCR analysis were used to assess the effects of each treatment on extracellular matrix remodeling and inflammation in skin on days 28 and 50 postirradiation. GFs induced the early development of thick, fully regenerated epidermis, skin appendages, and hair follicles, earlier than MSCs did. The acceleration of wound healing by GFs involved rearrangement of the deposited collagen, modification of the Col/MMP/TIMP balance, and modulation of the expression and localization of tenascin-C and of the expression of growth factors (VEGF, EGF, and FGF7). As MSC treatment did, GF injection decreased the irradiation-induced inflammatory response and switched the differentiation of macrophages toward an M2-like phenotype, characterized by CD163 + macrophage infiltration and strong expression of arginase-1. These findings indicate that GFs are an attractive target for regenerative medicine, for easier to collect, can grow in culture, and promote cutaneous wound healing in irradiation burn lesions.

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Impaired balance of type I and type III procollagen mRNA in cultured fibroblasts of patients with incisional hernia

Cited by 107 publications

References 27 publications

Prospective and retrospective study of incisional hernias in a tertiary care hospital

Prospective and retrospective study of incisional hernias in a tertiary care hospital

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

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