Impacts of silver nanoparticles on enzymatic activities, nitrifying bacteria, and nitrogen transformation in soil amended with ammonium and nitrate

Liu, Xiaohong; Wang, Juan; Wu, Lingli; Zhang, Li; Si, Youbin

doi:10.1016/s1002-0160(21)60036-x

Cited by 21 publications

(10 citation statements)

References 49 publications

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“…For example, nitrification is carried out by ammonia‐oxidizing archaea (AOA) and ammonia‐oxidizing bacteria (AOB), and can be indicated by nitrification functional genes such as amoA . However, the target fragment of bacterial amoA is 491 bp (Rotthauwe et al, 1997), and the length of archaeal amoA is 635 bp (Francis et al, 2005; Liu et al, 2021). Similarly, nosZ gene, an important functional gene of denitrification, is more than 700 bp (Highton et al, 2016; Jones et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment

Wang

et al. 2022

Molecular Ecology Resources

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Quantitative real‐time PCR (qPCR) has been widely used in quantifying bacterial and fungal populations in various ecosystems, as well as the fungi to bacteria ratio (F:B ratio). Recently, researchers have begun to apply droplet digital PCR (ddPCR) to this area; however, no study has systematically compared qPCR and ddPCR for quantitating both bacteria and fungi in environmental samples at the same time. Here, we designed probe‐primer pair combinations targeting the 16S rRNA gene and internal transcribed spacer (ITS) for the detection of bacteria and fungi, respectively, and tested both SYBR Green and TaqMan approaches in qPCR and ddPCR methods for mock communities and in real environmental samples. In mock communities, the quantification results of ddPCR were significantly closer to expected values (p < .05), and had smaller coefficients of variations (p < .05) than qPCR, suggesting ddPCR was more accurate and repeatable. In environmental samples, ddPCR consistently quantified ITS and 16S rRNA gene concentrations in all four habitats without abnormal overestimation or underestimation, and the F:B ratio obtained by ddPCR was consistent with phospholipid fatty acid analysis. Our results indicated that ddPCR had better precision, repeatability, sensitivity, and stability in bacterial and fungal quantitation than qPCR. Although ddPCR has high cost, complicated processes and restricted detection range, it shows insensitivity to PCR inhibitors and the potential of quantifying long target fragments. We expect that ddPCR, which is complementary to qPCR, will contribute to microbial quantification in environmental monitoring and evaluation.

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Section: Discussionmentioning

confidence: 99%

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment

Wang

et al. 2022

Molecular Ecology Resources

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“…178 Moreover, a number of studies suggest that the presence of Ag and its salts in soil significantly impairs the nitrogen cycle. [179][180][181][182] Many other studies reveal that NPs harm beneficial microbial cells through a variety of exterior and interior toxicity mechanisms. Additionally, NPs have an inhibiting effect on unicellular, mycorrhizal, and cellulose/lignin-degrading fungi.…”

Section: Current Challenges and Future Prospectsmentioning

confidence: 99%

Biosynthesized metal oxide nanoparticles for sustainable agriculture: next-generation nanotechnology for crop production, protection and management

2022

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“…However, their study did not indicate the optimum dose of silver and further study is required to confirm the optimum dose of silver in a real distribution system. Liu et al [ 50 ] and Choi and Hu [ 48 ] also found that the abundance of AOB and NOB decreased in the presence of silver nanoparticles. In contrast, some studies found that THMs have the potential to inhibit the nitrification process via the products by cometabolism reactions [ 110 , 138 ].…”

Section: Rapid Chloramine Decay and Nitrification Control Measuresmentioning

confidence: 99%

“…It has been found that nitrifiers’ growth is inhibited by element toxicity. Metals such as zinc (Zn), copper (Cu), and silver (Ag) can inhibit nitrification to some extent [ 47 , 48 , 49 , 50 , 51 , 52 ]. On the other hand, the application of low levels of phosphate (

) (usually less than 5 μg

) is found to inhibit nitrification [ 53 ].…”

Section: Introductionmentioning

confidence: 99%

Review of Nitrification Monitoring and Control Strategies in Drinking Water System

Hossain

Chow

Cook

et al. 2022

IJERPH

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Nitrification is a major challenge in chloraminated drinking water systems, resulting in undesirable loss of disinfectant residual. Consequently, heterotrophic bacteria growth is increased, which adversely affects the water quality, causing taste, odour, and health issues. Regular monitoring of various water quality parameters at susceptible areas of the water distribution system (WDS) helps to detect nitrification at an earlier stage and allows sufficient time to take corrective actions to control it. Strategies to monitor nitrification in a WDS require conducting various microbiological tests or assessing surrogate parameters that are affected by microbiological activities. Additionally, microbial decay factor (Fm) is used by water utilities to monitor the status of nitrification. In contrast, approaches to manage nitrification in a WDS include controlling various factors that affect monochloramine decay rate and ammonium substrate availability, and that can inhibit nitrification. However, some of these control strategies may increase the regulated disinfection-by-products level, which may be a potential health concern. In this paper, various strategies to monitor and control nitrification in a WDS are critically examined. The key findings are: (i) the applicability of some methods require further validation using real WDS, as the original studies were conducted on laboratory or pilot systems; (ii) there is no linkage/formula found to relate the surrogate parameters to the concentration of nitrifying bacteria, which possibly improve nitrification monitoring performance; (iii) improved methods/monitoring tools are required to detect nitrification at an earlier stage; (iv) further studies are required to understand the effect of soluble microbial products on the change of surrogate parameters. Based on the current review, we recommend that the successful outcome using many of these methods is often site-specific, hence, water utilities should decide based on their regular experiences when considering economic and sustainability aspects.

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Impacts of silver nanoparticles on enzymatic activities, nitrifying bacteria, and nitrogen transformation in soil amended with ammonium and nitrate

Cited by 21 publications

References 49 publications

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment

ddPCR surpasses classical qPCR technology in quantitating bacteria and fungi in the environment

Biosynthesized metal oxide nanoparticles for sustainable agriculture: next-generation nanotechnology for crop production, protection and management

Review of Nitrification Monitoring and Control Strategies in Drinking Water System

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