Impacts of Silver Nanoparticle Ingestion on Pigmentation and Developmental Progression in Drosophila

Key, State; Reaves, Denise K.; Turner, Fran; Bang, John J.

doi:10.5147/ajb.v1i3.10

Cited by 18 publications

(2 citation statements)

References 48 publications

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“…Cuticular pigmentation is a critical parameter of an insect’s life, as it influences various physiological and behavioral aspects, including lifespan, immunity, stress tolerance, and courtship. Loss of cuticular melanization is a well-known manifestation of AgNPs exposure in Drosophila [ 44 , 45 , 46 ]. Importantly, it was observed that the extent of loss of pigmentation in flies upon NP ingestion is directly correlated with decreased longevity, decreased locomotor activity, and overall toxicity.…”

Section: Resultsmentioning

confidence: 99%

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An In Vitro and In Vivo Study of the Efficacy and Toxicity of Plant-Extract-Derived Silver Nanoparticles

Desai

Singh

Edis

et al. 2022

JFB

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Silver nanoparticles (AgNPs) display unique plasmonic and antimicrobial properties, enabling them to be helpful in various industrial and consumer products. However, previous studies showed that the commercially acquired silver nanoparticles exhibit toxicity even in small doses. Hence, it was imperative to determine suitable synthesis techniques that are the most economical and least toxic to the environment and biological entities. Silver nanoparticles were synthesized using plant extracts and their physico-chemical properties were studied. A time-dependent in vitro study using HEK-293 cells and a dose-dependent in vivo study using a Drosophila model helped us to determine the correct synthesis routes. Through biological analyses, we found that silver nanoparticles’ cytotoxicity and wound-healing capacity depended on size, shape, and colloidal stability. Interestingly, we observed that out of all the synthesized AgNPs, the ones derived from the turmeric extract displayed excellent wound-healing capacity in the in vitro study. Furthermore, the same NPs exhibited the least toxic effects in an in vivo study of ingestion of these NPs enriched food in Drosophila, which showed no climbing disability in flies, even at a very high dose (250 mg/L) for 10 days. We propose that stabilizing agents played a superior role in establishing the bio-interaction of nanoparticles. Our study reported here verified that turmeric-extract-derived AgNPs displayed biocompatibility while exhibiting the least cytotoxicity.

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Section: Resultsmentioning

confidence: 99%

“…It is generally accepted that the extent of AgNP-mediated loss of melanization is proportional to the extent of system dysfunctions observed in Drosophila [ 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

An In Vitro and In Vivo Study of the Efficacy and Toxicity of Plant-Extract-Derived Silver Nanoparticles

Desai

Singh

Edis

et al. 2022

JFB

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Toxic and Genotoxic Effects of Silver Nanoparticles in Drosophila

Alaraby

Romero

Hernández

et al. 2018

Environ and Mol Mutagen

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The in vivo model Drosophila melanogaster was used here to determine the detrimental effects induced by silver nanoparticles (AgNPs) exposure. The main aim was to explore its interaction with the intestinal barrier and the genotoxic effects induced in hemocytes. The observed effects were compared with those obtained by silver nitrate, as an agent acting via the release of silver ions. Larvae were fed in food media containing both forms of silver. Results indicated that silver nitrate was more toxic than AgNPs when the viability “egg‐to‐adult” was determined. Depigmentation was observed in adults including those exposed to nontoxic concentrations, as indicative of exposure action. Interestingly, AgNPs were able to cross the intestinal barrier affecting hemocytes that show significant increases in the levels of intracellular reactive oxygen species. Additionally, significant levels of genotoxic damage, as determined by the comet assay, were also induced. When the expression of different stress‐response genes was determined, for both AgNPs and silver nitrate, significant upregulation of Sod2 and p53 genes was observed. Our results confirm for the first time that in an in vivo model as Drosophila, AgNPs are able to cross the intestinal barriers and produce primary DNA damage (comet assay) via oxidative stress induction. In general, the effects induced by silver nitrate were more pronounced than those induced by AgNPs what would emphasize the role of silver ions in the observed effects. Environ. Mol. Mutagen. 60:277–285, 2019. © 2018 Wiley Periodicals, Inc.

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Combating silver nanoparticle‐mediated toxicity in Drosophila melanogaster with curcumin

Singh

Raj

Padmanabhan

et al. 2020

J of Applied Toxicology

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Nanoscale materials display unique physical and chemical properties that enable their assimilation into a variety of industrial and consumer products. Amongst the widely used nanomaterials, silver nanoparticles (AgNPs) have gained tremendous recognition for various applications, owing to their extraordinary plasmonic and bactericidal properties. Despite of the extensive usage of AgNPs in various sectors, its impact on human health remains ambiguous. Several studies have established that higher doses of AgNPs are detrimental to organismal health. In order to attain the best from these versatile nanoparticles, a recent advent of green nanotechnology, that is, employment of metal nanoparticles synthesized using plant extracts, has emerged. Here, using Drosophila as a model system, we tested if adding curcumin, a biologically active polyphenolic compound present in turmeric, having multitudes of therapeutic properties, could mitigate AgNP‐mediated biotoxicity. We found that co‐administration of AgNPs with curcumin in the fly food could alleviate several harmful effects evoked by AgNPs ingestion in Drosophila model. Addition of curcumin superseded reduction in feeding, pupation, eclosion, pigmentation, and fertility caused by AgNPs ingestion. Interestingly, impairment in ovary development observed in flies reared on AgNPs‐supplemented food was also partially restored by co‐administration of AgNPs with curcumin. Furthermore, substantial alleviation of reactive oxygen species level and cell death was observed in larval tissues upon co‐supplementation of AgNPs with curcumin. We therefore propose that curcumin, when administered with AgNPs, can abrogate the toxic manifestations of AgNPs ingestion and hence can be incorporated in various consumer products encompassing it.

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Impacts of Silver Nanoparticle Ingestion on Pigmentation and Developmental Progression in Drosophila

Cited by 18 publications

References 48 publications

An In Vitro and In Vivo Study of the Efficacy and Toxicity of Plant-Extract-Derived Silver Nanoparticles

An In Vitro and In Vivo Study of the Efficacy and Toxicity of Plant-Extract-Derived Silver Nanoparticles

Toxic and Genotoxic Effects of Silver Nanoparticles in Drosophila

Combating silver nanoparticle‐mediated toxicity in Drosophila melanogaster with curcumin

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