Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level

Abstract

Biotechnological approaches using genetic modifications such as homologous gene overexpression can be used to decode gene functions under well-defined circumstances. However, only the recording of the resulting phenotypes allows inferences about the impact of the modification on the organisms’ evolutionary, ecological or economic performance. We here compare a potato wild-type cell line with two genetically engineered cell cultures homologously overexpressing Pathogenesis Related Protein 10a (pr-10a). A detailed analysis of the relative gene-expression patterns of pr-10a and its regulators sebf and pti4 over time provides insights into the molecular response of heterotrophic cells to distinct osmotic and salt-stress conditions. Furthermore, this system serves as an exemplar for the tracing of respiration kinetics as a faster and more sensitive alternative to the laborious and time-consuming recording of growth curves. The utility and characteristics of the resulting data type and the requirements for its appropriate analysis are figured out. It is demonstrated how this novel type of phenotypic information together with the gene-expression-data provides valuable insights into the effect of genetic modifications on the behaviour of cells on both the molecular and the macroscopic level.

Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N. tabacum

“…3b ). We also identified an up-regulated homolog ( c51305.g_c0 ) of tyrosine aminotransferase (TAT) gene known for SA synthesis, and an up-regulated pathogen-related gene PR-10 homolog ( c67327.g_c0 ) in the SA pathway, consistent with that reported previously 49 . The homolog of transcription factor TGA known to be involved in the SA pathway 50 was down-regulated.…”

Complete genome sequence of the marine methyl-halide oxidizing Leisingera methylohalidivorans type strain (DSM 14336T), a representative of the Roseobacter clade

“…Expectedly [30], on some substrates respiration was detected by phenotype microarray analysis even though these substrates did not sustain growth.…”

Rice Salt-Tolerance Enhancement by Expression of 1-Aminocyclopropane-1- Carboxylic Acid Oxidase Gene from Salt Tolerant Barley

“…Proteome analysis demonstrated that roots of Pisum sativum, a salinity-tolerant peanut callus cell line, had significantly elevated concentrations of PR-10 protein [36] as did salt-tolerant barley [21]. Overexpression of potato PR-10a gene in potato suspension cells and PR-10a gene from Jatropha curcas in tobacco exhibited significantly increased salt and osmotic tolerance [37,38]. Most PR proteins are induced through the plant hormones in addition to abiotic stress such as salt and drought and biotic stressors such as pathogens [39].…”