Impacts of Non-Lethal High-Temperature Stress on the Development and Reproductive Organs of Bradysia odoriphaga

Abstract

Bradysia odoriphaga is an agricultural pest in China’s vegetable industry. In this study, pupae and adults were exposed to various non-lethal high-temperatures. The results demonstrated a decreased rate of eclosion once the pupae were exposed to temperatures exceeding 37 °C for 1 h. No effect on the lifespan of unmated female adults was observed after exposure to temperature stress, while unmated male adult lifespan decreased (>37 °C for 2 h). The size of the testis and ovaries for unmated male and female adults decreased, as did the fecundity and egg hatching rate for mated females. Compared with the control group (25 °C), the testis size of unmated male adults decreased after high-temperature stress followed by recovery at 25 °C for 1 h, though the size of the ovaries of female adults did not change. Additionally, the size of the testis and ovaries for unmated male and female adults decreased following high-temperature stress and 24 h of recovery at 25 °C. High temperatures affected males more than females; 37 °C is the critical temperature to control the population of B. odoriphaga. These results lay the foundation for the future development of environmentally friendly high-temperature prevention and pest-control strategies.

First evidence that interactions of Heat Shock Protein 20 with reproduction-related proteins aid in fertility recovering from heat stress in Monochamus alternatus

“…Here, we found that by the age of 16 days, under normal environmental conditions, both the testes in males and ovaries in females were found to be fully mature, containing well-developed sperm and eggs, respectively. Previously researches revealed that high temperature damaged the development of reproductive organs in many insects, such as Bradysia odoriphaga, Drosophila suzukii and Agasicles hygrophila (Hu et al, 2022;Green et al, 2019;Zhao et al, 2016). In this study, we similarly found that exposing newly emerged adults of M. alternatus to heat stress for a duration of fteen days adversely affected the development of their reproductive organs.…”

The pine sawyer beetle alters oviposition strategy in response to extreme high temperatures

“…The reproductive organs of certain insect species undergo gradual development and maturation during the pupal stage. Previous studies have indicated that subjecting the pupae of these insects to non-lethal high-temperature stress has a greater impact on adult reproductive traits compared to applying heat stress to adult insects (Walsh et al 2021;Hu et al 2022). All these ndings make it reasonable to hypothesize that the stage at which insect reproductive organs mature is more susceptible to high temperatures in relation to reproductive traits.…”

Heat stress‐induced oviposition behavioral change correlates with sperm damage in the pine sawyer beetle, Monochamus alternatus

“…Previous studies have indicated that subjecting the pupae of these insects to nonlethal high-temperature stress had a greater impact on adult reproductive traits compared to applying heat stress to adult insects. 32,33 All of these findings made it reasonable to hypothesize that the stage at which insect reproductive organs mature was more susceptible to high temperatures about reproductive traits.…”