Impacts of Nitrogen Starvation on Expression Profiles of Involving Genes in Triacylglycerol Biosynthesis in Chlamydomonas reinhardtii: Towards Microalgal Systems Biotechnology
Abstract:Objective: Chlamydomonas reinhardtii, the most commonly studied model organism in microalgal kingdom, is known to be a rich lipid producer. Methods: Here, a naturally isolated C. reinhardtii strain was cultivated phototrophically at Erlenmeyer scale in BG11 culture medium. After reaching to stationary growth phase, a nitrogen limited regime with different nitrogen concentrations was applied. Results: Quantitative real time PCR analysis of DGTT1 and DGTT2 genes involved in triacylglycerol accumulation confirmed… Show more
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