Impacts of nitrogen-containing coagulants on the nitritation/denitrification of anaerobic digester centrate

Abstract

Heterotrophic nitrification is linked to the use of nitrogen-containing coagulants added to enhance centrifugablity of biosolids.

Robust Nitritation of Anaerobic Digester Centrate Using Dual Stressors and Timed Alkali Additions

“…16S rRNA community analysis results revealed 5 clones in the SS/SBR and 15 clones in the DS/SBR. A possible explanation is heterotrophic nitrification mediated by ammonia-oxidizing Xanthomonadaceae, as previously reported for SBRs operated at low DO and fed with ADC amended with nitrogen-containing coagulants …”

“…Table summarizes changes in the number of dominant 16S rRNA and amoA clones (see Figures S1 and S2 for detailed resolution of the clone libraries). Compared to the inoculum, both the SS/SBR and the DS/SBR were enriched in Nitrosomonadaceae, a family that includes many AOB, and in Xanthomonadaceaee, a family that includes heterotrophic AOB. − Compared to the day 0 inoculum, the number of AOB clones in the family Nitrosomonadaceae nearly doubled in the SS/SBR (from 3 to 5), but in the DS/SBR, the increase was 5-fold (from 3 to 15) (Table ). The amo A clone library confirmed 16S rRNA data for Nitrosomonadaceae, suggesting selection for Nitrosomonas IWT514, a single species that accounted for 61 of the 62 amoA clones (98%) in the DS/SBR.…”

“…A possible explanation is heterotrophic nitrification mediated by ammonia-oxidizing Xanthomonadaceae, as previously reported for SBRs operated at low DO 69 and fed with ADC amended with nitrogencontaining coagulants. 70 On day 100, cycle performance was evaluated for both the SS/SBR and DS/SBR. For the SS/SBR, NOB were initially inhibited, and NO 2 − −N accumulated to 200 mg/L during a period of elevated FA at the initiation of the cycle (Figure 2C-1).…”

Characterization of the nitrogen-transforming microbial community in the biofilms of a full-scale rotating biological contactor system treating wastewater from a fresh market building

“…Chitinophagaceae are known as heterotrophic AOB, which were previously found in engineered environments. 49,50 Nitrosomonas, autotrophic AOB, were present at approximately 1% relative abundance in both samples, T1 and T2 (Fig. 3 and 4).…”

CFD-accelerated bioreactor optimization: reducing the hydrodynamic parameter space

“…Microbial processes play an integral role in the removal of organic carbon and nutrients. 6–8 The prevailing technology first developed at the turn of the 20th century is activated sludge, a process that has since been modified to enable nutrient removal. Many emerging technologies cannot cross the “Valley of Death” because they treat tiny flows (in many cases, just milliliters per day) while adoption in practical applications may require treatment of tens of millions of liters per day.…”