Impacts of long-term exposure to tetracycline and sulfamethoxazole on the sludge granules in an anoxic-aerobic wastewater treatment system

Du, Bingbing; Yang, Qi; Li, Xunan; Yuan, Wei; Chen, Yulong; Wang, Ruifei

doi:10.1016/j.scitotenv.2019.05.313

Cited by 59 publications

(13 citation statements)

References 53 publications

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“…Thiothrix and Sphaerotilus are both well-known filamentous bacteria that are involved in sludge bulking, and have been validated as the main bulking causative bacteria in the type of A-O wastewater treatment system examined in this study [31,54,55]. The abundance of the sul2 gene showed an obvious tendency to increase when exposed to antibiotics in system B, which was possibly related to the higher percentage of Thiothrix that resulted from the exposure of microorganisms to TC and SMX (Figure 3 and Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…Experiments were conducted in two identical laboratory-scale A-O systems (System A and System B) as shown schematically in Figure 1. The detailed information was described previously [25,31]. System A was set as the control, while system B was fed with 5 mg·L −1 TC from day 41 to 200 and a combination of 5 mg·L −1 TC and 1 mg·L −1 SMX from day 241 to 420.…”

Section: Methodsmentioning

confidence: 99%

“…Primer sequences targeting these genes are listed in Table 1 and these were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). The qPCR reaction was performed on the LightCycler ® 96 qPCR System (F. Hoffmann-La Roche AG, Basel, Switzerland) with SYBR ® Green PCR master mix (Vazyme, Nanjing, China) following the protocol reported previously [31,39]. In brief, the qPCR reaction mixture (20.0 μL) contained 10.0 μL SYBR ® Green Master Mix, 0.4 μL forward/reverse primer (10 μM), 2.0 μL template DNA, and 7.2 μL double distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…SMX was then chosen as another representative antibiotic for the season that it was the most widely found sulfonamides in wastewater treatment and has ecological toxicity to the environment [3,12]. Previous studies have investigated the effects of sub-inhibition dosages of TC and SMX exposure on the performances and activated sludge characteristics in a laboratory-scale anoxic-aerobic (A-O) wastewater treatment system [25,31]. The results indicated that although there were no significant effects on the main pollutant removal (such as chemical oxygen demand and total nitrogen), long-term exposure to TC alone or to TC combined with SMX did affect the sludge settling performance by affecting the compositions of microbial communities.…”

Section: Introductionmentioning

confidence: 99%

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Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole

Yang

Ruifei

et al. 2019

IJERPH

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The removal of antibiotics and widespread of antibiotic resistance genes (ARGs) have received continuous attention due to the possible threats to environment. However, little information is available on the evolution of antibiotic resistance and the relationship between ARGs and microbial communities under long-term exposure to sub-inhibitory concentrations of antibiotics. In our study, two laboratory-scale anoxic-aerobic wastewater treatment systems were established and operated for 420 days to investigate the evolution of antibiotic resistance under exposure of 5 mg·L−1 tetracycline (TC) or 5 mg·L−1 TC and 1 mg·L−1 sulfamethoxazole (SMX). The average removal rates of TC and SMX were about 59% and 72%, respectively. The abundance of the main ARGs responsible for resistance to TC and SMX increased obviously after antibiotics addition, especially when TC and SMX in combination (increased 3.20-fold). The tetC and sul1 genes were the predominant genes in the development of TC and SMX resistance, in which gene sul1 had the highest abundance among all the detected ARGs. Network analysis revealed that under antibiotic pressure, the core bacterial groups carrying multiple ARGs formed and concentrated in about 20 genera such as Dechloromonas, Candidatus Accumulibacter, Aeromonas, Rubrivivax, in which intI1 played important roles in transferring various ARGs except sul3.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole

Yang

Ruifei

et al. 2019

IJERPH

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“…Because of their unreasonable use and abuse, due to the recent increasing demands of livestock production, TCs have received increasing attention as an important class of contaminants of emerging concern (CEC). TCs pose potential hazards both environmentally and health wise, including the increase in multi-resistant bacterial strains, bioaccumulation and biomagnification in aquatic organisms, and can be carcinogenic to human beings upon long-term exposure [1][2][3][4]. Residual TCs could be absorbed by plants and livestock, and then transferred into human beings through the food chain, representing a major threat to human health.…”

Section: Introductionmentioning

confidence: 99%

A Mild Method for Preparation of Highly Selective Magnetic Biochar Microspheres

Zhao

Rong-qi

Wang

2020

IJMS

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We report the use of biochar and Fe3O4 nanoparticles as co-stabilizers for oil-in-water (o/w) Pickering emulsion. The emulsion is subsequently used to prepare magnetic tetracycline-imprinted biochar composite microspheres (MMIPMs) with good uniformity and high selectivity. The MMIPMs were characterized by scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) measurements, Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and thermogravimetry analysis (TGA). The adsorption properties of tetracycline to the MMIPMs were investigated using different adsorption experiments including adsorption kinetic experiment, equilibrium binding experiment, selectivity evaluation and competitive adsorption tests. The theoretical maximum adsorption capacity of the MMIPMs (15.45 mg g−1) was greater than that of the raw biochar (2.10 mg g−1) and non-imprinted biochar composite microspheres (3.39 mg g−1) for tetracycline. Further, the MMIPMs were used as adsorbent for magnetic solid phase extraction (SPE) for the extraction of tetracycline present in drinking water, milk, fish and chicken samples. Under optimal conditions, the results showed good recovery yield ranging from 88.41% to 106.29% with a relative standard deviation (RSD) ranging from 0.35% to 6.83%, respectively.

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Effect of sulfamethoxazole on nitrate removal by simultaneous heterotrophic aerobic denitrification

Progress

Zhao

Wang

et al. 2022

Water Environment Research

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The increase in mariculture activities worldwide has not only led to a rise of nitrogen compounds in the ecosystem but has also intensified the accumulation of antibiotics in both terrestrial and marine environments. This study focused on the effect of typical antibiotics, specifically sulfamethoxazole (SMX) on nitrate removal from mariculture wastewater by aerobic denitrification process; an aerobic denitrification system feeding with 148.2 mg/L COD, 8.59 mg/L nitrate, 0.72 mg/L nitrite, and 4.75 mg/L ammonium was set up. The hydraulic retention time (HRT) was 8 h. As the aerobic bioreactor started up successfully without SMX dosage, an excellent removal of ammonium, nitrite, and nitrate was achieved at 91.35%, 93.33%, and 88.51%, respectively; the corresponding effluent concentrations were 0.41 mg/L, 0.048 mg/L, and 0.96 mg/L. At the influent SMX doses of 0, 1, 5, and 10 mg/L, the COD removal reached 96.91%, 96.27%, 88.69%, and 85.89%, resulting in effluent concentrations of 4.53, 5.45, 17.38, and 20.6 mg/L, respectively. Nitrification was not inhibited by SMX dosage. However, aerobic denitrification was inhibited by 10 mg/L SMX. Proteobacteria was the most abundant phylum, and surprisingly its abundance increased with the increase in SMX concentration. An excellent SMX degradation was noted at initial SMX dosages of 1, 5, and 10 mg/L; the removal rate was 100%,100%, and 99.8%, respectively. The SMX degrading genera Comamonas sp., Acinetobacter sp., and Thauera sp. are of great validity to wastewater engineers because they have demonstrated efficiency in simultaneous heterotrophic aerobic denitrification and antibiotic degradation as well as COD removal. Practitioner Points Nitrification was not inhibited by increase in SMX dosage. An increase in SMX dosage inhibited aerobic denitrification. COD removal was not affected by increased SMX dosage. Comamonas, Acinetobacter, and Thauera had high efficiency in COD removal and SMX degradation.

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Impacts of long-term exposure to tetracycline and sulfamethoxazole on the sludge granules in an anoxic-aerobic wastewater treatment system

Cited by 59 publications

References 53 publications

Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole

Evolution of Antibiotic Resistance and the Relationship between the Antibiotic Resistance Genes and Microbial Compositions under Long-Term Exposure to Tetracycline and Sulfamethoxazole

A Mild Method for Preparation of Highly Selective Magnetic Biochar Microspheres

Effect of sulfamethoxazole on nitrate removal by simultaneous heterotrophic aerobic denitrification

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