Climate change and global rapid agricultural expansion have drastically reduced the area of wetlands globally recently, so that the ecosystem functions of wetlands have been impacted severely. Therefore, this study integrated the land use data and the integrated valuation of ecosystem services and tradeoffs (InVEST) model to evaluate the impacts of the land-use change (LUC) on wetland ecosystem services (ES) from 1976 to 2016 in the Tumen River Basin (TRB). Results reveal that the area of wetlands in TRB had decreased by 22.39% since 1976, mainly due to the rapid conversion of wetlands to dry fields and construction lands, and the LUC had induced notable geospatial changes in wetland ES consequently. A marked decrease in carbon storage and water yield was observed, while the habitat quality was enhanced slightly. Specifically, the conversion of rivers and paddy fields to ponds and reservoirs were the main reasons for the increase in habitat quality and caused the habitat quality to increase by 0.09. The conversion of marshes to lakes, paddy fields, grasslands, dry fields, and artificial surfaces were the key points for the decline in carbon storage; the conversion of marshes to lakes (5.38 km2) and reservoir ponds (1.69 km2) were the dominant factors driving the losses of water yield. According to our results, we should center on the conservation of wetlands and rethink the construction of the land use. The findings are expected to provide a theoretical reference and basis for promoting environmental protection in TRB and the construction of ecological civilization in border areas.