Impacts of Gadolinium and Yttrium on the Performance and Microbial Community Composition of a Bench-Scale-Activated Sludge System

Salmon, O. E.; Rasmussen, Kalen; Aurelius, Stephanie; Vanzin, Gary; Munakata-Marr, Junko; Fujita, Yoshiko

doi:10.1021/acsestwater.2c00098

Cited by 3 publications

(2 citation statements)

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“…The Kruskal-Wallis and pairwise Wilcoxon tests were used to determine alpha diversity statistical significances (adjusted value of p <0.05), along with the Wilcoxon effect size. To quantitatively assess between sample diversity, samples were first bootstrap rarefied with replacement (63 trials) to a sequencing depth of 1,080 as previously performed (Salmon et al, 2022). Bray-Curtis Dissimilarity distance matrices were constructed using the R package Vegan, and beta diversity plots were constructing using ggplot2 and loess regression for line fitting (Bray and Curtis, 1957;Wickham, 2016;Oksanen et al, 2022).…”

Section: Amplicon Sequence Processing and Analysismentioning

confidence: 99%

Spatial and temporal dynamics at an actively silicifying hydrothermal system

et al. 2023

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Steep Cone Geyser is a unique geothermal feature in Yellowstone National Park (YNP), Wyoming, actively gushing silicon-rich fluids along outflow channels possessing living and actively silicifying microbial biomats. To assess the geomicrobial dynamics occurring temporally and spatially at Steep Cone, samples were collected at discrete locations along one of Steep Cone’s outflow channels for both microbial community composition and aqueous geochemistry analysis during field campaigns in 2010, 2018, 2019, and 2020. Geochemical analysis characterized Steep Cone as an oligotrophic, surface boiling, silicious, alkaline-chloride thermal feature with consistent dissolved inorganic carbon and total sulfur concentrations down the outflow channel ranging from 4.59 ± 0.11 to 4.26 ± 0.07 mM and 189.7 ± 7.2 to 204.7 ± 3.55 μM, respectively. Furthermore, geochemistry remained relatively stable temporally with consistently detectable analytes displaying a relative standard deviation <32%. A thermal gradient decrease of ~55°C was observed from the sampled hydrothermal source to the end of the sampled outflow transect (90.34°C ± 3.38 to 35.06°C ± 7.24). The thermal gradient led to temperature-driven divergence and stratification of the microbial community along the outflow channel. The hyperthermophile Thermocrinis dominates the hydrothermal source biofilm community, and the thermophiles Meiothermus and Leptococcus dominate along the outflow before finally giving way to more diverse and even microbial communities at the end of the transect. Beyond the hydrothermal source, phototrophic taxa such as Leptococcus, Chloroflexus, and Chloracidobacterium act as primary producers for the system, supporting heterotrophic growth of taxa such as Raineya, Tepidimonas, and Meiothermus. Community dynamics illustrate large changes yearly driven by abundance shifts of the dominant taxa in the system. Results indicate Steep Cone possesses dynamic outflow microbial communities despite stable geochemistry. These findings improve our understanding of thermal geomicrobiological dynamics and inform how we can interpret the silicified rock record.

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Section: Amplicon Sequence Processing and Analysismentioning

confidence: 99%

Spatial and temporal dynamics at an actively silicifying hydrothermal system

et al. 2023

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“…The Kruskal-Wallis and pairwise Wilcoxon tests were used to determine alpha diversity statistical significances (adjusted pvalue < 0.05), along with the Wilcoxon effect size. To quantitatively assess between sample diversity, samples were first bootstrap rarefied with replacement (63 trials) to a sequencing depth of 1080 as previously performed (Salmon et al, 2022). Bray-Curtis Dissimilarity distance matrices were constructed using the R package Vegan, and beta diversity plots were constructing using ggplot2 and loess regression for line fitting (Bray and Curtis, 1957;Oksanen et al, 2022;Wickham, 2016).…”

Section: Amplicon Sequence Processing and Analysismentioning

confidence: 99%

Spatial and Temporal Dynamics at an Actively Silicifying Hydrothermal System

Rasmussen

Stamps

Vanzin

et al. 2023

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Steep Cone Geyser is a unique geothermal feature in Yellowstone National Park, Wyoming (YNP), actively gushing silicon rich fluids along outflow channels possessing living and actively silicifying microbial biomats. To assess the geomicrobial dynamics occurring temporally and spatially at Steep Cone, samples were collected at discrete locations along one of Steep Cones outflow channels for both microbial community composition and aqueous geochemistry analysis during field campaigns in 2010, 2018, 2019, and 2020. Geochemical analysis characterized Steep Cone as an oligotrophic, surface boiling, silicious, alkaline-chloride thermal feature with consistent dissolved inorganic carbon and total sulfur concentrations down the outflow channel ranging from 4.59 +/- 0.11 to 4.26 +/- 0.07 mM and 189.7 +/- 7.2 to 204.7 +/- 3.55 uM respectively. Furthermore, geochemistry remained relatively stable temporally with consistently detectable analytes displaying a relative standard deviation < 32%. A thermal gradient decrease of ~55C was observed from the sampled hydrothermal source to the end of the sampled outflow transect (90.34C +/- 3.38 to 35.06C +/- 7.24). The thermal gradient led to temperature-driven divergence and stratification of the microbial community along the outflow channel. The hyperthermophile Thermocrinis dominates the hydrothermal source biofilm community, and the thermophiles Meiothermus and Leptococcus dominate along the outflow before finally giving way to more diverse and even microbial communities at the end of the transect. Beyond the hydrothermal source, phototrophic taxa such as Leptococcus, Chloroflexus, and Chloracidobacterium act as primary producers for the system supporting heterotrophic growth of taxa such as Raineya, Tepidimonas, and Meiothermus. Community dynamics illustrate large changes yearly driven by abundance changes of the dominant taxa in the system. Results indicate Steep Cone possesses dynamic outflow microbial communities despite stable geochemistry. These findings improve our understanding of thermal geomicrobiological dynamics and inform how we can interpret the silicified rock record.

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