Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice

Chen, Miao; Fan, Huimin; Ledford, Benjamin T.; Farah, Zayd; Barron, Catherine; Liu, Zhongmin; He, Jia‐Qiang

doi:10.1016/j.mvr.2016.12.006

Cited by 9 publications

(9 citation statements)

References 32 publications

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“…Surgical procedures range from a single ligation of the femoral or iliac artery [579–581] to a complete excision of the artery [576] and sometimes even the vein and the nerve were dissected as well [582, 583]. Several excellent review papers have been written on these variations in the hind limb ischemia model [584–587]. When choosing a particular variant of the mouse hind limb ischemia model for a study, it is important to define the goal of the study.…”

Section: Mouse Hind Limb Ischemia Modelmentioning

confidence: 99%

Consensus guidelines for the use and interpretation of angiogenesis assays

et al. 2018

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The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

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Section: Mouse Hind Limb Ischemia Modelmentioning

confidence: 99%

Consensus guidelines for the use and interpretation of angiogenesis assays

et al. 2018

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“…With its increasing prevalence, PAD, a new threat to public health beyond coronary artery disease, involves aberrant vascular endothelial cell proliferation and angiogenesis ( 2 , 7 , 18 ). Patients with PAD may develop MALE during the late stage of disease propagation, which markedly reduces blood flow to the extremities and progresses to the point of severe resting pain and even tissue loss ( 2 , 18 ). Although surgical or endovascular revascularization has been used for the treatment of PAD, some patients fail to tolerate these intricate and high-risk surgeries ( 2 ).…”

Section: Discussionmentioning

confidence: 99%

Deletion of MicroRNA-21 Impairs Neovascularization Following Limb Ischemia: From Bedside to Bench

Chang

Lin

Huang

et al. 2022

Front. Cardiovasc. Med.

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With an increasing prevalence, peripheral arterial disease (PAD), cause by atherosclerosis is a new threat to public health beyond coronary artery disease and involves aberrant vascular endothelial cell proliferation and angiogenesis. The degree of vascular remodeling is influenced by the processes described. MicroRNA-21 (miR-21) has been found to play a critical role in cellular functions, including angiogenesis. Nevertheless, the effect of miR-21 on endothelial cells in response to hypoxia is largely unknown. Using wild-type C57BL/6J and miR-21–/– mice, we compared the capability of angiogenesis in response to hindlimb hypoxic/ischemia. In an in vitro study, we further studied whether overexpression of miR-21 mitigates hypoxia-induced apoptosis and impaired angiogenesis. Also, we prospectively collected the sera of patients with limb ischemia and followed the clinical information, including major adverse limb events (MALEs). Using laser Doppler perfusion imaging and CD31 staining, compared with miR-21–/– mice, wild-type mice expressed a significantly higher capability of angiogenesis and less apoptosis following 28 days of hindlimb hypoxic/ischemic surgery. In our in vitro study, after 24 h of hypoxia, proliferation, migration, and tube formation were significantly impaired in cells treated with the miR-21 inhibitor but rescued by the miR-21 mimic. Mechanistically, by suppressing PTEN/PI3K/AKT, miR-21 promoted angiogenesis and suppressed apoptosis in endothelial cells post hypoxia. In patients with limb ischemia, the high expression of circulating miR-21 was associated with less subsequent MALE. Collectively, miR-21 could be a biomarker associated with the endogenous ability of angiogenesis and reflect subsequent MALE in patients. Additionally, abolishing miR-21 impairs angiogenesis and promotes apoptosis post limb ischemia. Further studies are required to elucidate the clinical applications of miR-21.

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“…With its increasing prevalence, PAD, a new threat to public health beyond coronary artery disease, involves aberrant vascular endothelial cell proliferation and angiogenesis [2,4,12]. Patients with PAD may develop MALE during the late stage of disease propagation, which markedly reduces blood flow to the extremities and progresses to the point of severe resting pain and even tissue loss [4,12]. Although surgical or endovascular revascularization has been used for the treatment of PAD, some patients fail to tolerate these intricate and high-risk surgeries [4].…”

Section: Discussionmentioning

confidence: 99%

“…The surgery of limb ischemia in mice 17 Young (10-week-old) and aged (80-100-week-old) mice were randomly assigned to (1) control young group, (2) control aged group, (3) young hindlimb ischemia group, (4) aged hindlimb ischemia group. As described previously, a model of hindlimb ischemia were established in the left hind limbs of mice [12,33]. Briefly, mice were anesthetized with an intraperitoneal injection of pentobarbital (80 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

Effects of STAT3 on aging-dependent neovascularization impairment following limb ischemia: from bedside to bench

Chang

Hong

Huang

et al. 2022

Aging

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Aging is a major risk factor for ischemic hypoxia-related diseases, including peripheral artery diseases (PADs). Signal transducer and activator of transcription 3 (STAT3) is a critical transcription activator in angiogenesis. Nevertheless, the effect of aging on endothelial cells and their responses to hypoxia are not well studied. Using a hindlimb hypoxic/ischemic model of aged mice, we found that aged mice (80-100-week-old) expressed significantly lower levels of angiogenesis than young mice (10-week-old). In our in vitro study, aged endothelial cells (≥30 passage) showed a significant accumulation of β-galactosidase and a high expression of aging-associated genes, including p16, p21, and hTERT compared with young cells (<10 passage). After 24 hours of hypoxia exposure, proliferation, migration and tube formation were significantly impaired in aged cells compared with young cells. Notably, STAT3 and angiogenesis-associated proteins such as PI3K/AKT were significantly downregulated in aged mouse limb tissues and aged cells. Further, using STAT3 siRNA, we found that suppressing STAT3 expression in endothelial cells impaired proliferation, migration and tube formation under hypoxia. Correspondingly, in patients with limb ischemia we also observed a higher expression of circulating STAT3, associated with a lower rate of major adverse limb events (MALEs). Collectively, STAT3 could be a biomarker reflecting the development of MALE in patients and also a regulator of age-dependent angiogenesis post limb ischemia. Additional studies are required to elucidate the clinical applications of STAT3.

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Impacts of femoral artery and vein excision versus femoral artery excision on the hindlimb ischemic model in CD-1 mice

Cited by 9 publications

References 32 publications

Consensus guidelines for the use and interpretation of angiogenesis assays

Consensus guidelines for the use and interpretation of angiogenesis assays

Deletion of MicroRNA-21 Impairs Neovascularization Following Limb Ischemia: From Bedside to Bench

Effects of STAT3 on aging-dependent neovascularization impairment following limb ischemia: from bedside to bench

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