Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

Li, Yang; Liang, Shusheng; Shao, Yuchao; Li, Yuanyou; Chen, Cuiying; You, Cuihong; Monroig, Óscar; Tocher, Douglas R.; Wang, Shuqi

doi:10.1016/j.aquaculture.2021.737113

Cited by 24 publications

(11 citation statements)

References 83 publications

(111 reference statements)

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“…The proper dietary lipid and protein ratio supports higher growth rates, partly based on protein-sparing in many species, including blunt snout bream [2]. However, the present study showed that dietary 11% lipid significantly suppress the specific growth rate of fish, which is similar to results obtained in blunt snout bream, golden pompano (Trachinotus ovatus), and largemouth bass (Micropterus salmoides) fed with 11%, 18%, and 18% fat diet, respectively [3,26,29,30]. From the perspective of energy utilization, a possible reason may be that excessively high, dietary fat-driven energy intake results in the imbalance of metabolism and uptake of other nonlipid nutrients of fish [31,32].…”

Section: Discussionsupporting

confidence: 80%

“…As is well known, dietary lipid level is closely associated with fish whole-body lipid content, and an excessive lipid diet is generally accompanied with increased fat deposition in the liver, viscera, and muscle [29,34,35]. The lipid homeostasis of the liver is maintained by the uptake, secretion, and transport of lipid.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicated that dietary pantothenic acid can regulate lipid metabolism in blunt snout bream fed with high-fat diet. Generally, a high-fat diet is easier to generate more reactive oxygen species (ROS) that cause oxidative stress and oxidative destruction of cellular membranes [29,42]. Fortunately, fish have the enzymatic and nonenzymatic antioxidant system, including SOD, CAT, glutathione peroxidase (GSH-Px), and GSH, which have been confirmed to protect cells and tissues against ROS [43].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Pantothenic Acid on Growth Performance, Oxidative Stress, and Lipid Content in High-Fat-Fed Megalobrama amblycephala

Tang

Zhang

et al. 2022

Aquaculture Nutrition

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This study was to elucidate the effects of dietary pantothenic acid (PA) on growth, lipid metabolism, and antioxidant status in juvenile blunt snout bream Megalobrama amblycephala. Fish were fed with four experimental diets containing two lipid levels (5% and 11%) and two PA supplementation levels (24 mg kg-1 and 240 mg kg-1) for eight weeks. The results showed that diets containing 11% lipid significantly increased condition factor, mesenteric fat index, triacylglycerol (TG), and cholesterol (TCH) in the liver as well as total lipid content and TCH in the muscle ( P < 0.05 ), but significantly decreased specific growth rate and whole-body protein ( P < 0.05 ). As the dietary lipid level increased, hepatic antioxidant enzyme activities were significantly increased ( P < 0.05 ) as well as malondialdehyde content ( P < 0.05 ). Besides, cytosolic cytochrome c concentration was also significantly ( P < 0.05 ) increased, while mitochondrial protein, cytochrome c, and Na+-K+-ATPase activities were significantly decreased ( P < 0.05 ). There were no significant changes on growth performance and body composition of PA supplementation. Then, significantly negative ( P < 0.05 ) interactions were observed between dietary lipid and PA supplementation on EPA, DHA, and EPA + DHA content of the muscle. Saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) contents in the liver were significantly increased ( P < 0.05 ) with dietary lipid level increased, while liver monounsaturated fatty acid (MUFA) was significantly decreased ( P < 0.05 ). Muscle SFA, DHA, and EPA + DHA contents were significantly decreased ( P < 0.05 ) when dietary fat level increased. Meanwhile, EPA, DHA, and EPA + DHA content was significantly decreased ( P < 0.05 ) with dietary high PA supplementation. On the whole, a high PA supplementation level of 240 mg kg-1 could alleviate lipid content accumulated and oxidative damage induced by a high-fat diet.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of Pantothenic Acid on Growth Performance, Oxidative Stress, and Lipid Content in High-Fat-Fed Megalobrama amblycephala

Tang

Zhang

et al. 2022

Aquaculture Nutrition

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“…In addition, lipid accumulation could be observed after chronic feeding with a high-fat diet of fish because fatty acid oxidation, as well as fatty acid transport capacity, is inhibited [13][14][15]. Furthermore, highcarbohydrate and high-fat diets can lead to oxidative stress [16][17][18][19] and poor immune function in fish [20][21][22][23]. Hepatic lipid dysregulation, oxidative stress, and proinflammatory cytokines act synergistically to trigger hepatic lipid accumulation [24,25].…”

Section: Introductionmentioning

confidence: 99%

Effects of Genistein on Lipid Metabolism, Antioxidant Activity, and Immunity of Common Carp (Cyprinus carpio L.) Fed with High-Carbohydrate and High-Fat Diets

Yang

Zhao

et al. 2023

Aquaculture Nutrition

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A 56-day feeding trial was conducted to investigate the effects of genistein on growth, lipid metabolism, antioxidant capacity, and immunity of common carp fed with high-carbohydrate or high-fat diets. Five diets were used to feed fish: control diet (5% fat; CO), high-fat diet (11% fat; HF), high-carbohydrate diet (45% carbohydrate; HC), and HF or HC diet with 500 mg/kg genistein (FG or CG). Results showed that final body weight (FW) and specific growth rate (SGR) were significantly reduced, but the supplementation with genistein resulted in higher values of FW and SGR than the HF or HC group. Both high carbohydrate and high fat belong to high-energy diets, which may promote lipid deposition. Genistein obviously decreased liver triglyceride (TG) content and alleviated hepatic fat vacuolation in the HF and HC groups. The expression of lipid metabolism genes (cpt-1 and atgl) was markedly higher in the FG group than in the HF group. The lipid synthesis-related genes (fas, acc, and pparγ) were elevated in high-energy diets but recovered to the control level or reduced after genistein treatments. With respect to fatty acid transporter genes, fatp increased in the FG group, and cd36 increased in the CG group. Furthermore, the antioxidant and immune indexes, such as total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), acid phosphatase (ACP), and lysozyme (LZM) activities, were decreased, while malonate aldehyde (MDA) content, activities of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were enhanced in the HF and HC groups. The antioxidant and immunity values could be ameliorated by treatment with genistein. Moreover, the transcript levels of antioxidant-related genes (cat, gr, and nrf2) in the liver and anti-inflammatory factors (tgf-β and il-10) and lyz in the head kidney tissue were promoted, although the expression levels of proinflammatory factors (tnf-α and il-6) declined in the genistein supplementation group, which confirmed the antioxidant and immune-enhancing effects of genistein. Therefore, 500 mg/kg genistein could ameliorate the negative effects of high-energy diets on immunity.

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“…Inflammatory responses are regulated by a combination of anti-inflammatory cytokines (IL-10, TGF-β, etc.) ( 30 , 62 ) and pro-inflammatory cytokines (IL-1β, IL-8, TNF-α, etc.) ( 30 , 48 , 63 ), which in turn are typically modulated through the NF-κB/IκB/IKK signaling pathway ( 64 , 65 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of cysteine addition to low-fishmeal diets on the growth, anti-oxidative stress, intestine immunity, and Streptococcus agalactiae resistance in juvenile golden pompano (Trachinotus ovatus)

Liu

Zhu²,

Guo³

et al. 2022

Front. Immunol.

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As the precursor of taurine, cysteine serves physiological functions, such as anti-oxidative stress and immune improvement. Investigation of cysteine and its derivatives has made positive progress in avian and mammalian species, yet the study and application of cysteine in aquatic animals are relatively rare. Therefore, we evaluated the effects of supplementing a low-fishmeal diet with various levels of cysteine on the growth, antioxidant capacity, intestine immunity, and resistance against Streptococcus agalactiae of the juvenile golden pompano (Trachinotus ovatus). According to our study, exogenous supplementation with 0.6-1.2% cysteine greatly increased the final body weight (FBW) and specific growth rate (SGR) of golden pompano compared to the control group. Under the present conditions, the optimum dietary cysteine supplementation level for golden pompano was 0.91% based on the polynomial regression analysis of SGR. Meanwhile, we found that the Nrf2/Keap1/HO-1 signaling pathway was notably upregulated with the increase of exogenous cysteine, which increased antioxidant enzyme activity in serum and gene expression in the intestine and reduced the level of reactive oxygen species (ROS) in the serum of golden pompano. In addition, morphological analysis of the midgut demonstrated that exogenous cysteine improved muscle thickness and villi length, which suggested that the physical barrier of the intestine was greatly strengthened by cysteine. Moreover, cysteine increased the diversity and relative abundance of the intestinal flora of golden pompano. Cysteine suppressed intestinal NF-κB/IKK/IκB signaling and pro-inflammatory cytokine mRNA levels. Conversely, intestinal anti-inflammatory cytokine gene expression and serum immune parameters were upregulated with the supplementary volume of cysteine and improved intestine immunity. Further, exogenous cysteine supplementation greatly reduced the mortality rate of golden pompano challenged with S. agalactiae. In general, our findings provide more valuable information and new insights into the rational use of cysteine in the culture of healthy aquatic animals.

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Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

Cited by 24 publications

References 83 publications

Effects of Pantothenic Acid on Growth Performance, Oxidative Stress, and Lipid Content in High-Fat-Fed Megalobrama amblycephala

Effects of Pantothenic Acid on Growth Performance, Oxidative Stress, and Lipid Content in High-Fat-Fed Megalobrama amblycephala

Effects of Genistein on Lipid Metabolism, Antioxidant Activity, and Immunity of Common Carp (Cyprinus carpio L.) Fed with High-Carbohydrate and High-Fat Diets

Effects of cysteine addition to low-fishmeal diets on the growth, anti-oxidative stress, intestine immunity, and Streptococcus agalactiae resistance in juvenile golden pompano (Trachinotus ovatus)

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