Impacts of carbon nanotubes on biochemical reactions: insight into interaction between carbon nanotubes and DNA polymerase enzyme

Employment of nanomaterials in polymerase chain reaction: insight into the impacts and putative operating mechanisms of nano-additives in PCR

“…It has been well-proven in the literature that ssDNA and DNA polymerase enzyme bind on nanomaterial surface via p-p stacking, surface charge facilitated interactions or van der Waals' forces. 59,78,90,130,131 It was reported long before that AuNPs-modied with ssDNA can distinguish a perfect complementary strand from a single base mutated sequence, 132 which could rationalize the enhanced specicity in nanomaterial-assisted PCR. However, it could be wise to optimize the nanomaterial concentration so that they cannot offer a huge surface where the all reactants concentrate at once and stop reacting.…”