Impacts after inhalation of nano- and fine-sized titanium dioxide particles: morphological changes, translocation within the rat lung, and evaluation of particle deposition using the relative deposition index

Eydner, Maja; Schaudien, Dirk; Creutzenberg, Otto; Ernst, Heinrich; Hansen, Tanja; Baumgärtner, Wolfgang; Rittinghausen, Susanne

doi:10.3109/08958378.2012.697494

Cited by 27 publications

(26 citation statements)

References 54 publications

(86 reference statements)

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“…Van Ravenzwaay et al also reported nano-TiO 2 (14% rutile, 86% anatase) accumulation in lymphoid tissue after inhalation exposure in the rat [20]. These results indicated that inhaled nano-TiO 2 could translocate throughout the body quickly and tend to accumulate in immune organ, which possibly through uptake by migratory antigen presenting cells, because many toxicological studies have observed that macrophages or foreign-body giant cells appeared in lung tissue as exposure doses increased [21,22]. …”

Section: Discussionmentioning

confidence: 99%

Systemic Immune Effects of Titanium Dioxide Nanoparticles after Repeated Intratracheal Instillation in Rat

Zhang

Chang

et al. 2014

IJMS

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The potential immune effects of titanium dioxide nanoparticles (nano-TiO2) are raising concern. Our previous study verified that nano-TiO2 induce local immune response in lung tissue followed by intratracheal instillation administration. In this study, we aim to evaluate the systemic immune effects of nano-TiO2. Sprague Dawley rats were treated by intratracheal instillation with nano-TiO2 at doses of 0.5, 4, and 32 mg/kg body weight, micro-TiO2 with 32 mg/kg body weight and 0.9% NaCl, respectively. The exposure was conducted twice a week, for four consecutive weeks. Histopathological immune organs from exposed animals showed slight congestion in spleen, generally brown particulate deposition in cervical and axillary lymph node. Furthermore, immune function response was characterized by increased proliferation of T cells and B cells following mitogen stimulation and enhanced natural killer (NK) cell killing activity in spleen, accompanying by increased number of B cells in blood. No significant changes of Th1-type cytokines (IL-2 and INF-γ) and Th2-type cytokines (TNF-α and IL-6) were observed. Intratracheal exposure to nano-TiO2 may be one of triggers to be responsible for the systemic immune response. Further study is needed to confirm long-lasting lymphocyte responses and the potential mechanisms.

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Section: Discussionmentioning

confidence: 99%

Systemic Immune Effects of Titanium Dioxide Nanoparticles after Repeated Intratracheal Instillation in Rat

Zhang

Chang

et al. 2014

IJMS

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“…For the nanoparticle pair TiO 2 P25 and TiO 2 T805, a 0.15 w-% phosphate buffer was chosen that had been successfully used in a nose-only experiment in rats to generate a partially nanoscaled TiO 2 P25 aerosol (Creutzenberg et al, 2009;Eydner et al, 2012). In case of TiO 2 T805 (hydrophobic surface properties because of surface-treatment with organics) Tween® 80 was used as an additional detergent auxiliary (Table 2).…”

Section: Preparation Of Particle Suspensionsmentioning

confidence: 99%

Change in agglomeration status and toxicokinetic fate of various nanoparticlesin vivofollowing lung exposure in rats

Creutzenberg

Bellmann

Korolewitz

et al. 2012

Inhalation Toxicology

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The deposition characteristics in lungs following inhalation, the potential toxic effects induced and the toxicokinetic fate including a possible translocation to other sites of the body are predominantly determined by the agglomeration status of nanoscaled primary particles. Systemic particle effects, i.e. effects on remote organs besides the respiratory tract are considered to be of relevant impact only for de-agglomerated particles with a nanoscaled aspect. Rats were exposed to various types of nanoscaled particles, i.e. titanium dioxide, carbon black and constantan. These were dispersed in physiologically compatible media, e.g. phosphate buffer, sometimes including auxiliaries. Rats were treated with aqueous nanoparticle dispersions by intratracheal instillation or were exposed to well-characterized nanoparticle aerosols. Subsequently, alterations in the particle size distribution were studied using transmission electron microscopy (TEM) as well as the bronchoalveolar lavage (BAL) technique. Based on the results in various approaches, a tendency of nanoscaled particles to form larger size agglomerates following deposition and interaction with cells or the respiratory tract is predominant. The contrary trend, i.e. the increase of particle number due to a disintegration of agglomerates seems not to be of high relevance.

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“…reported that exposure to TiO 2 by intraperitoneal injection induced obvious congestion and lymph nodule proliferation in the mouse spleen [61]. These results indicated that exposure to TiO 2 particles could translocate throughout the body quickly and tend to accumulate in immune organs, possibly through uptake by migratory antigen presenting cells, because many toxicological studies have observed that macrophages or foreign-body giant cells appeared in lung tissue as exposure doses increased [62]. …”

Section: Discussionmentioning

confidence: 99%

Immunotoxicity evaluation of novel bioactive composites in male mice as promising orthopaedic implants

El‐Bassyouni

Eshak

Barakat

et al. 2017

cejoi

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ObjectiveIn orthopaedics, novel bioactive composites are largely needed to improve the synthetic achievement of the implants. In this work, semiconducting metal oxides such as SiO2, TiO2, and ZrO2 particles (Ps) were used individually and in different ratios to obtain different biphasic composites. The immunotoxicity of these composites was tested to inspect the potential toxicity prior to their use in further medical applications.Materials and methodsIn vitro mineralisation ability was inspected by soaking the composites in simulated body fluid (SBF). Additionally, in vivo experiments were performed consuming male mice using ISSR-PCR, micronucleus (MN) test, comet assay, glutathione peroxidase activity, and determination of albumin, globulin, lymphocyte population, ALT, and AST levels. Several groups of adult male albino mice were treated with 100, 200, and 400 mg/kg body weight of SiO2, TiO2, and ZrO2-Ps in pure or mixed forms.ResultsOur findings revealed that treatment of mice with low and medium doses of SiO2, TiO2, and ZrO2-Ps in pure or mixed form revealed values relatively similar to the control group. However, using 400 mg/kg especially from TiO2-Ps in genuine form or mixed with SiO2 showed proliferation in the toxicity rates compared with the high dose of SiO2 and ZrO2-Ps.ConclusionsThe results suggest that TiO2 composite induced in vivo toxicity, oxidative DNA damage, bargain of the antioxidant enzymes, and variations in the levels of albumin, globulin, lymphocyte population, ALT, and AST in a dose-dependent manner. However, SiO2, and ZrO2 composites revealed a lower toxicity in mice compared with that of TiO2.

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Impacts after inhalation of nano- and fine-sized titanium dioxide particles: morphological changes, translocation within the rat lung, and evaluation of particle deposition using the relative deposition index

Cited by 27 publications

References 54 publications

Systemic Immune Effects of Titanium Dioxide Nanoparticles after Repeated Intratracheal Instillation in Rat

Systemic Immune Effects of Titanium Dioxide Nanoparticles after Repeated Intratracheal Instillation in Rat

Change in agglomeration status and toxicokinetic fate of various nanoparticlesin vivofollowing lung exposure in rats

Immunotoxicity evaluation of novel bioactive composites in male mice as promising orthopaedic implants

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