To analyze limb pain in tethered dairy cows, we compared three cow floor mat materials (pasture mat ® [PM], KKM rubber mat ® [RM], and woodchip [WC]) with soil ground considering the pressure applied to the anterior knee of Holstein cows. Three Holstein dry cows (791.3 AE 44.6 kg, 3.8 AE 1.1 years old) moored at the Education