Impact of wastewater on the microbial diversity of periphyton and its tolerance to micropollutants in an engineered flow-through channel system

Carles, Louis; Wullschleger, Simon; Joss, Adriano; Eggen, Rik I.L.; Schirmer, Kristin; Schuwirth, Nele; Stamm, Christian; Tlili, Ahmed

doi:10.1016/j.watres.2021.117486

Cited by 34 publications

(75 citation statements)

References 60 publications

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“…In November the concentration of almost all ATBs was significantly higher than during other months. Some previous works had already pointed out the presence of similar concentrations of pharmaceuticals (including AZM, CPR, CLR, LVX, NFX, SMX and TMP) in river biofilms ( Aubertheau et al, 2017 ; Auguet et al, 2017 ; Carles et al, 2021 ; Valdés et al, 2021 ) and also observed variations in the distribution of these compounds over time ( Valdés et al, 2021 ).…”

Section: Resultsmentioning

confidence: 95%

“…Some previous works had already pointed out the presence of pharmaceuticals (including AZM, CPR, CLR, LVX, NFX, SMX and TMP) (Aubertheau et al, 2017;Auguet et al, 2017;Carles et al, 2021;Valdés et al, 2021) and MGEs (integrons) in similar samples (Aubertheau et al, 2017;Auguet et al, 2017;Chen et al, 2020;Li and Zhang, 2020). However, it was not clear how the presence of integrons can be linked to the presence of ATBs, as integrons also contain genes other than ARGs or encoding proteins of unknown function.…”

Section: What Potential Risk Does River Biofilm Contamination Pose?mentioning

confidence: 99%

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River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

et al. 2022

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Continuous exposure to low concentrations of antibiotics (sub-minimal inhibitory concentration: sub-MIC) is thought to lead to the development of antimicrobial resistance (AMR) in the environmental microbiota. However, the relationship between antibiotic exposure and resistance selection in environmental bacterial communities is still poorly understood and unproven. Therefore, we measured the concentration of twenty antibiotics, resistome quality, and analyzed the taxonomic composition of microorganisms in river biofilms collected upstream (UPS) and downstream (DWS) (at the point of discharge) from the wastewater treatment plant (WWTP) of Poitiers (France). The results of statistical analysis showed that the antibiotic content, resistome, and microbiome composition in biofilms collected UPS were statistically different from that collected DWS. According to Procrustes analysis, microbial community composition and antibiotics content may be determinants of antibiotic resistance genes (ARGs) composition in samples collected DWS. However, network analysis showed that the occurrence and concentration of antibiotics measured in biofilms did not correlate with the occurrence and abundance of antibiotic resistance genes and mobile genetic elements. In addition, network analysis suggested patterns of co-occurrence between several ARGs and three classes of bacteria/algae: Bacteroidetes incertae sedis, Cyanobacteria/Chloroplast, and Nitrospira, in biofilm collected UPS. The absence of a direct effect of antibiotics on the selection of resistance genes in the collected samples suggests that the emergence of antibiotic resistance is probably not only due to the presence of antibiotics but is a more complex process involving the cumulative effect of the interaction between the bacterial communities (biotic) and the abiotic matrix. Nevertheless, this study confirms that WWTP is an important reservoir of various ARGs, and additional efforts and legislation with clearly defined concentration limits for antibiotics and resistance determinants in WWTP effluents are needed to prevent their spread and persistence in the environment.

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Section: Resultsmentioning

confidence: 95%

Section: What Potential Risk Does River Biofilm Contamination Pose?mentioning

confidence: 99%

River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

et al. 2022

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“…The experiment was conducted in a flow-through channel system called “Maiandros” that mimics natural conditions in freshwater streams but allows for targeted manipulations. Initially described by Burdon et al (2020), this system was subsequently equipped with a light system to ensure a photoperiod of 12h light: 12h dark (Carles et al, 2021), as well as with an automated-online monitoring system for parameters such as flow rate, water conductivity and temperature (Desiante et al, 2022). The ultra-filtration (UF) unit added to the system consisted of a membrane module of 50 m 2 surface and a nominal pore size of 0.4 μm, allowing for the removal of particulates (including microorganisms) from the effluent, with a microorganism removal efficiency of 99.3% (Desiante et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

“…The selection criteria included detection frequency, concentrations in municipal wastewater, toxicity, analytical limitations and substance groups. Passive samplers from the channels were prepared and extracted according to Moschet et al (2015) with few modifications (Carles et al, 2021). Micropollutant concentrations in the passive sampler extracts were then used to derive theoretical concentrations in the water by using the sampling rates (R S ) established by Moschet et al (2015).…”

Section: Methodsmentioning

confidence: 99%

“…Micropollutants were also analysed in the passive sampler extract used for the community tolerance bioassays – see Tlili et al (2017) for the detailed description of the extraction method. Concentrations of micropollutants that accumulated in periphyton were measured after extraction by a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method as described by Munz et al (2018) with some modifications (Carles et al, 2021). Micropollutant analysis in all samples was performed by HPLC-MS/MS as described by Carles et al (2021).…”

Section: Methodsmentioning

confidence: 99%

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Wastewater microorganisms impact microbial diversity and important ecological functions of stream periphyton

Carles¹,

Wullschleger²,

Joss³

et al. 2022

Preprint

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Wastewater treatment plant effluents can impact microbial communities in receiving streams. However, little is known about the role of microorganisms in wastewater as opposed to other wastewater constituents, such as nutrients and micropollutants. We aimed therefore at determining the impact of wastewater microorganisms on the microbial diversity and function of periphyton, key microbial communities in streams. Periphyton was grown in flow-through channels that were continuously alimented with a mixture of stream water and unfiltered or ultra-filtered wastewater. Impacts were assessed on periphyton biomass, activities and tolerance to micropollutants, as well as on microbial diversity. Our results showed that wastewater microorganisms colonized periphyton and modified its community composition, resulting for instance in an increased abundance of Chloroflexi and a decreased abundance of diatoms and green algae. This led to shifts towards heterotrophy, as suggested by the changes in nutrient stoichiometry and the increased mineralization potential of carbon substrates. An increased tolerance towards micropollutants was only found for periphyton exposed to unfiltered wastewater but not to ultra-filtered wastewater, suggesting that wastewater microorganisms were responsible for this increased tolerance. Overall, our results highlight the need to consider the role of wastewater microorganisms when studying potential impacts of wastewater on the receiving water body.

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Microbial community structure and diversity attached to the periphyton in different urban aquatic habitats

Yang,

Zhou,

Pang

et al. 2024

Environ Monit Assess

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Impact of wastewater on the microbial diversity of periphyton and its tolerance to micropollutants in an engineered flow-through channel system

Cited by 34 publications

References 60 publications

River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

Wastewater microorganisms impact microbial diversity and important ecological functions of stream periphyton

Microbial community structure and diversity attached to the periphyton in different urban aquatic habitats

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