Impact of wall modifications on protein elution in high performance capillary zone electrophoresis

Maa, Yih-Fen; Hyver, Karen J.; Swedberg, Sally A.

doi:10.1002/jhrc.1240140117

Cited by 41 publications

(8 citation statements)

References 6 publications

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“…However, Eq. (1) points to another mechanism, namely viscosity increase in the electric double layer close to the wall, e.g., due to a voluminous coating [13]. To enhance this effect, the immobilized polymer has to be solvated by the solvent.…”

Section: Introductionmentioning

confidence: 98%

“…Some are based on silanization of surface silanol groups with alkyl silanes [14][15][16][17], others involve derivatization with anchor groups for a consecutive polymerization or polymer immobilization step. Anchor groups can be glycidsilanes for polyethylene glycol coatings [18], aminosilanes for maltopolysaccharides via reductive amination [19], or for protein binding after glutardialdehyde modification [13] as well as methacryloyltrimethoxysilane for polyacrylamide [20], and vinylsilanes for polymerization of acrylamide [21,22] or vinyl acetate to prepare PVA coatings [23,24]. A noncovalent immobilization of PVA was published by Gilges et al [25].…”

Section: Introductionmentioning

confidence: 99%

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Control of electroosmotic flow in nonaqueous capillary electrophoresis by polymer capillary coatings

Steiner

Hassel

2003

Electrophoresis

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In aqueous capillary electrophoresis the electroosmotic flow (EOF) can be strongly suppressed or eliminated by coating the capillary surface silanols either by buffer additive adsorption or chemical modification. Hydrophilic coatings, e.g., polyvinyl alcohol (PVA) proved to be most efficient for EOF control in applications like DNA analysis. In nonaqueous capillary electrophoresis (NACE), however, the EOF cannot be totally suppressed with these capillaries and coating efficiency turned out to be solvent-depending. In this paper, fused-silica capillaries with monomeric and polymeric coatings differing in hydrophobicity and chemical properties (vinyl, vinyl acetate, vinyl alcohol and acrylates with different alkyl chain length) were investigated. Besides studying the EOF characteristics with different organic solvents and water, gas chromatography (GC) measurements were carried out to probe the silanol reduction via ether retention and the surface hydrophobicity by retention of nonane. Good correlations between GC results and EOF magnitude could be found. It could be demonstrated that the polymeric coating has to be solvatized by the buffer solvent to reduce the EOF. The PVA coating was optimal for aqueous systems but not effective for some nonaqueous buffers. On the other hand, polyvinyl acetate and polyethyl acrylate as polymeric coatings proved to be optimal to reduce the EOF in NACE.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Control of electroosmotic flow in nonaqueous capillary electrophoresis by polymer capillary coatings

Steiner

Hassel

2003

Electrophoresis

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“…The use of polymeric coatings has led to high efficiency and reproducible protein separations preventing the surface adsorptions. Polymers ranging from highly hydrophobic to highly hydrophilic were used for this purpose to obtain efficient separations of proteins in CE [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cifuentes

Poppe

Kraak

et al. 1996

Journal of Chromatography B: Biomedical Sciences and Applicatio

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“…Four different approaches have been reported and involve, briefly, (i) elimination of the interaction by adjusting the pH of the buffer to such a value that the silanol groups are non-charged [1]; (ii) adjusting the pH of the buffer of such a value that the charges of the silanol groups and protein have the same sign [2,3]; (iii) dynamic modification of the surface by neutral and cationic additives in the buffer [4][5][6][7][8][9]; and (iv) chemical modification using covalent bonding of the fusedsilica surface [10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Cellulose acetate-coated fused-silica capillaries for the separation of proteins by capillary zone electrophoresis

Busch

Kraak

Poppe

1995

Journal of Chromatography A

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Cellulose acetate-coated fused-silica capillaries for the separation of proteins by capillary zone electrophoresis Busch, M.H.A.; Kraak, J.C.; Poppe, H. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Thin-film coatings of cellulose acetate on the surface of fused-silica capillaries were tested for the separation of proteins by capillary zone electrophoresis. The coating procedure is very simple and only involves the filling of the capillary with cellulose acetate solution, followed by flushing the capillary with helium. The coating appears to mask the underlying silanol groups towards basic proteins effectively; column efficiencies up to 10 6 plates per metre were achieved for ribonuclease A. The high efficiency, the batch-to-batch and the run-to-run reproducibility and the long-term stability of the coating are advantageous features of the method. The coating procedure provides a simple, stable and easy to reproduce method of surface deactivation and can be applied with other cellulose derivatives such as cellulose triacetate or cross-linked hydroxypropylcellulose. The films can also be applied to shield the surface of hollow polypropylene fibres. Unfortunately, this skin coating is destroyed above pH 7.5 and therefore cannot be recommended for zone electrophoresis at alkaline pH or for isoelectric focusing.

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Impact of wall modifications on protein elution in high performance capillary zone electrophoresis

Cited by 41 publications

References 6 publications

Control of electroosmotic flow in nonaqueous capillary electrophoresis by polymer capillary coatings

Control of electroosmotic flow in nonaqueous capillary electrophoresis by polymer capillary coatings

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cellulose acetate-coated fused-silica capillaries for the separation of proteins by capillary zone electrophoresis

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