Impact of vineyard ground cover management on the occurrence and activity of entomopathogenic nematodes and associated soil organisms

Blanco-Pérez, Rubén; Sáenz-Romo, María Gloria; Vicente-Díez, Ignacio; Ibáñez-Pascual, Sergio; Martínez-Villar, Elena; Mancebón, Vicente Santiago Marco; Pérez-Moreno, Ignacio; Campos‐Herrera, Raquel

doi:10.1016/j.agee.2020.107028

Cited by 15 publications

(9 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enhancing good practices for pest, disease, and disease vector management would help address these challenges [40,54]. By maintaining the biodiversity of the vineyard agroecosystem, natural enemies of arthropods can contribute to crop protection [55][56][57]. In this study, we compared different EPN populations as facultative biological control agents against larval and pupal instars of the key grape pest, EGVM.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

Vicente-Díez

Blanco-Pérez

Chelkha

et al. 2021

Insects

Self Cite

View full text Add to dashboard Cite

The European grapevine moth (EGVM) Lobesia botrana (Lepidoptera: Tortricidae) is a relevant pest in the Palearctic region vineyards and is present in the Americas. Their management using biological control agents and environmentally friendly biotechnical tools would reduce intensive pesticide use. The entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are well-known virulent agents against arthropod pests thanks to symbiotic bacteria in the genera Xenorhabdus and Photorhabdus (respectively) that produce natural products with insecticidal potential. Novel technological advances allow field applications of EPNs and those bioactive compounds as powerful bio-tools against aerial insect pests. This study aimed to determine the viability of four EPN species (Steinernema feltiae, S. carpocapsae, S. riojaense, and Heterorhabditis bacteriophora) as biological control agents against EGVM larval instars (L1, L3, and L5) and pupae. Additionally, the bioactive compounds from their four symbiotic bacteria (Xenorhabdus bovienii, X. nematophila, X. kozodoii, and Photorhabdus laumondii subsp. laumondii, respectively) were tested as unfiltered ferment (UF) and cell-free supernatant (CFS) against the EGVM larval instars L1 and L3. All of the EPN species showed the capability of killing EGVM during the larval and pupal stages, particularly S. carpocapsae (mortalities of ~50% for L1 and >75% for L3 and L5 in only two days), followed by efficacy by S. feltiae. Similarly, the bacterial bioactive compounds produced higher larval mortality at three days against L1 (>90%) than L3 (~50%), making the application of UF more virulent than the application of CFS. Our findings indicate that both steinernematid species and their symbiotic bacterial bioactive compounds could be considered for a novel agro-technological approach to control L. botrana in vineyards. Further research into co-formulation with adjuvants is required to expand their viability when implemented for aboveground grapevine application.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

Vicente-Díez

Blanco-Pérez

Chelkha

et al. 2021

Insects

Self Cite

View full text Add to dashboard Cite

show abstract

“…The IJs were recovered in tap water upon emergence, stored at 12–14 °C, and used within two weeks after harvest [ 39 ]. We completed the molecular identification of all EPN populations following the procedures described by Blanco-Pérez et al [ 40 ]. Briefly, we mechanically disaggregated ~500 IJs employing sterile blue pestles assembled in a pellet mixer (VWR International, Lutterworth, UK).…”

Section: Methodsmentioning

confidence: 99%

Insecticidal Effect of Entomopathogenic Nematodes and the Cell-Free Supernatant from Their Symbiotic Bacteria against Philaenus spumarius (Hemiptera: Aphrophoridae) Nymphs

Vicente-Díez

Blanco-Pérez

González-Trujillo

et al. 2021

Insects

Self Cite

View full text Add to dashboard Cite

The meadow spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the primary vector of Xylella fastidiosa (Proteobacteria: Xanthomonadaceae) in Europe, a pest–disease complex of economically relevant crops such as olives, almonds, and grapevine, managed mainly through the use of broad-spectrum pesticides. Providing environmentally sound alternatives to reduce the reliance on chemical control is a primary challenge in the control of P. spumarius and, hence, in the protection of crops against the expansion of its associated bacterial pathogen. Entomopathogenic nematodes (EPNs) are well-known biocontrol agents of soil-dwelling arthropods. Recent technological advances in field applications, including improvements in obtaining cell-free supernatant from their symbiotic bacteria, allow their successful implementation against aerial pests. Thus, this study aimed to evaluate, for the first time, the efficacy of EPN applications against nymphal instars of P. spumarius. We tested four EPN species and the cell-free supernatant of their corresponding symbiotic bacteria: Steinernema feltiae–Xenorhabdus bovienii, S. carpocapsae–X. nematophila, S. riojaense–X. kozodoii, and Heterorhabditis bacteriophora–Photorhabdus laumondii subsp. laumondii. First, we showed that 24 and 72 h exposure to the foam produced by P. spumarius nymphs did not affect S. feltiae virulence. The direct application of steinernematid EPNs provided promising results, reaching 90, 78, and 53% nymphal mortality rates after five days of exposure for S. carpocapsae, S. feltiae, and S. riojaense, respectively. Conversely, the application of the cell-free supernatant from P. laumondii resulted in nymphal mortalities of 64%, significantly higher than observed for Xenorhabdus species after five days of exposure. Overall, we demonstrated the great potential of the application of specific EPNs and cell-free supernatant of their symbiont bacteria against P. spumarius nymphs, introducing new opportunities to develop them as biopesticides for integrated management practices or organic vineyard production.

show abstract

“…EPNs are distributed in soils throughout the world (Adams et al, 2006), including in commercial vineyards (Belair et al, 2001;Mracek et al, 2005;Campos-Herrera et al, 2008;Blanco-Pérez et al, 2020). EPN infectivity and survival ability are mediated by abiotic (type of soil, humidity, temperature, etc.…”

Section: Exploring the Possible Contribution Of Entomopathogenic Nema...mentioning

confidence: 99%

“…However, nowadays, the use of EPN is not restricted to the belowground application either to their use just as a biological control agent. Recent studies are providing evidence that the natural presence of EPN can be an indicator of the impact of soil management (Campos-Herrera et al, 2008;2014;Valadas et al, 2014;Majic et al, 2019;Blanco-Pérez et al, 2020). Hence, the framework of the use of EPN is now expanded to all components of the IPDM in a direct or indirect approach (Figure 2).…”

Section: Exploring the Possible Contribution Of Entomopathogenic Nema...mentioning

confidence: 99%

Positioning entomopathogenic nematodes for the future viticulture: exploring their use against biotic threats and as bioindicators of soil health

Campos‐Herrera¹,

Vicente-Díez²,

Blanco-Pérez³

et al. 2021

Turk J Zool

Self Cite

View full text Add to dashboard Cite

Vineyards face several biotic threats that compromise the grape quality and quantity. Among those that cause relevant economic impact and have worldwide distribution are the oomycete Plasmopara vitícola, the fungi Erysiphe necator and Botrytis cinerea, and the arthropods Lobesia botrana, Tetranychus urticae, and Phylaenus spumarius (principal vector of the bacterial disease Xylella fastidiosa in Europe). Their management relies primarily on agrochemicals with short persistence; widespread use of these chemicals causes environmental and human health problems. The challenge of sustainable viticulture is to provide ecologically sound alternatives. In this regard, the application of entomopathogenic nematodes (EPNs) and natural products derived from their symbionts can be an alternative. EPNs are well-known biocontrol agents for soil-dwelling insects. However, current research demonstrates the great potential of both EPN and their derivates as direct bio-tools against some of the key fungal and arthropods pests present aboveground. In addition, recent evidence shows that detecting EPN presence and activity and their relation with other soil organisms associated with them can help us to understand the impact of different agricultural practices on vineyard management. Altogether, this review illustrates the great potential of EPN to enhance pest and disease management in the next generation of viticulture.

show abstract

Impact of vineyard ground cover management on the occurrence and activity of entomopathogenic nematodes and associated soil organisms

Cited by 15 publications

References 66 publications

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

Exploring the Use of Entomopathogenic Nematodes and the Natural Products Derived from Their Symbiotic Bacteria to Control the Grapevine Moth, Lobesia botrana (Lepidoptera: Tortricidae)

Insecticidal Effect of Entomopathogenic Nematodes and the Cell-Free Supernatant from Their Symbiotic Bacteria against Philaenus spumarius (Hemiptera: Aphrophoridae) Nymphs

Positioning entomopathogenic nematodes for the future viticulture: exploring their use against biotic threats and as bioindicators of soil health

Contact Info

Product

Resources

About