Impact of TNF and IL-1β on capillary networks within engineered human adipose tissues

Proulx, Maryse; Safoine, Meryem; Mayrand, Dominique; Aubin, Kim; Maux, Amandine; Fradette, Julie

doi:10.1039/c6tb00265j

Cited by 6 publications

(12 citation statements)

References 76 publications

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“…The stability of the capillary networks in our tissues is particularly valuable for the in vitro evaluation of angiomodulatory molecules over many days [15].…”

Section: Discussionmentioning

confidence: 99%

“…This strategy can be applied to a multitude of substitutes such as reconstructed skin containing a microvascularized dermis [9,10], a field in which the Laboratoire d'organogénèse expérimentale (LOEX) center has been a pioneer [11][12][13]. Our team also developed an adipose tissue model featuring a capillary network in vitro [14,15], since the field of adipose tissue engineering would also greatly benefit from enhanced graft vascularization upon implantation.…”

Section: Introductionmentioning

confidence: 99%

“…The seeding of human microvascular endothelial cells (hMVECs) onto adipose sheets during the reconstruction enables the production of human reconstructed adipose tissue substitutes containing a preformed network of capillary-like structures [14]. Indeed, the natural human cells and matrix provide a biologically relevant environment conducive to the migration, proliferation, and tubule formation by adult hMVECs, leading to a capillary network that can be modulated by external angiogenic modulators [15].…”

Section: Introductionmentioning

confidence: 99%

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Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

et al. 2018

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Engineered adipose tissues are developed for their use as substitutes for tissue replacement in reconstructive surgery. To ensure a timely perfusion of the grafted substitutes, different strategies can be used such as the incorporation of an endothelial component. In this study, we engineered human adipose tissue substitutes comprising of functional adipocytes as well as a natural extracellular matrix using the self-assembly approach, without the use of exogenous scaffolding elements. Human microvascular

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“…The stability of the capillary networks in our tissues is particularly valuable for the in vitro evaluation of angiomodulatory molecules over many days [15].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

et al. 2018

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“…The above models primarily focused on adipocyte biology with and without endothelial cells. However, in 2016, Proulx et al analyzed the influence of adipocytes exposed to TNF and IL-1β on endothelial cells derived from human microvascular endothelial cells using the model established by Aubin et al [129,135]. Intriguingly, capillary-like structures made of endothelial cells exposed to TNF-α or IL-1β were shorter, less branched and were more apoptotic; demonstrating a valid model to study the crosstalk between adipocytes and endothelial cells.…”

Section: Human Vascular 3d Adipose Disease Modelsmentioning

confidence: 99%

In vitro tissue-engineered adipose constructs for modeling disease

2019

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Adipose tissue is a vital tissue in mammals that functions to insulate our bodies, regulate our internal thermostat, protect our organs, store energy (and burn energy, in the case of beige and brown fat), and provide endocrine signals to other organs in the body. Tissue engineering of adipose and other soft tissues may prove essential for people who have lost this tissue from trauma or disease. In this review, we discuss the applications of tissue-engineered adipose tissue specifically for disease modeling applications. We provide a basic background to adipose depots and describe three-dimensional (3D) in vitro adipose models for obesity, diabetes, and cancer research applications. The approaches to engineering 3D adipose models are diverse in terms of scaffold type (hydrogel-based, silk-based and scaffold-free), species of origin (H. sapiens and M. musculus) and cell types used, which allows researchers to choose a model that best fits their application, whether it is optimization of adipocyte differentiation or studying the interaction of adipocytes and other cell types like endothelial cells. In vitro 3D adipose tissue models support discoveries into the mechanisms of adipose-related diseases and thus support the development of novel anti-cancer or anti-obesity/diabetes therapies.

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“…It is possible to reconstruct adipose tissue by tissue engineering in a highly controlled environment. Such human reconstructed adipose tissues (hrAT) are interesting novel tools suitable for pharmaco-toxicolocal studies that could favorably replace the use of immortalized cell lines, monocultures or animal cells [22–24]. Indeed, these 3D models are particularly suited to study adipose tissue formation, maturation and remodelling in vitro , as well as the tissue-specific gene expression [24].…”

Section: Introductionmentioning

confidence: 99%

Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue

et al. 2019

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Reconstructed human adipose tissues represent novel tools available to perform in vitro pharmaco-toxicological studies. We used adipose-derived human stromal/stem cells to reconstruct, using tissue engineering techniques, such an adipose tridimensional model. To determine to what extent the in vitro model is representative of its native counterpart, adipogenic differentiation, triglycerides accumulation and phospholipids profiles were analysed. Ingenuity Pathway Analysis software revealed pathways enriched with differentially-expressed genes between native and reconstructed human adipose tissues. Interestingly, genes related to fatty acid metabolism were downregulated in vitro, which could be explained in part by the insufficient amount of essential fatty acids provided by the fetal calf serum used for the culture. Indeed, the lipid profile of the reconstructed human adipose tissues indicated a particular lack of linoleic acid, which could interfere with physiological cell processes such as membrane trafficking, signaling and inflammatory responses. Supplementation in the culture medium was able to influence the lipid profile of the reconstructed human adipose tissues. This study demonstrates the possibility to directly modulate the phospholipid profile of reconstructed human adipose tissues. This reinforces its use as a relevant physiological or pathological model for further pharmacological and metabolic studies of human adipose tissue functions.

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Impact of TNF and IL-1β on capillary networks within engineered human adipose tissues

Abstract: Inflammatory cytokines lead to capillary network disorganization and secreted factor modulation within human microvascularized engineered adipose tissues.

Cited by 6 publications

References 76 publications

Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

Short-term post-implantation dynamics of in vitro engineered human microvascularized adipose tissues

In vitro tissue-engineered adipose constructs for modeling disease

Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue

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