Impact of Time on Fluid Resuscitation with Hypertonic Saline (NaCl 7.5%) in Rats with LPS-Induced Acute Lung Injury

Petroni, Ricardo; Biselli, Paolo José Cesare; Lima, Thaís Martins de; Velasco, Irineu Tadeu; Soriano, Francisco

doi:10.1097/shk.0000000000000461

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Hypertonic solution acts on polymorphonuclear A2 adenosine receptors and causes a feedback mechanism that stimulates cAMP and PKA release, thus blocking neutrophil activation [ 41 , 42 ]. Nevertheless, the therapeutic window for a beneficial effect of fluid resuscitation with hypertonic fluid seemed to be very narrow [ 43 ] and may be related to leukocyte activation at the time of fluid use [ 44 ]. Our study confirms the beneficial impact of hypertonic solutions on the IL-6 release but not on TNFα.…”

Section: Discussionmentioning

confidence: 99%

Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study

Duburcq

Durand

Tournoys

et al. 2018

Ann. Intensive Care

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BackgroundSodium lactate seemed to improve fluid balance and avoid fluid overload. The objective of this study was to determine if these beneficial effects can be at least partly explained by an improvement in disseminated intravascular coagulation (DIC)-associated renal microvascular thrombosis.MethodsAncillary work of an interventional randomized open label controlled experimental study. Fifteen female “Large White” pigs (2 months old) were challenged with intravenous infusion of E. coli endotoxin. Three groups of five animals were randomly assigned to receive different fluids: a treatment group received sodium lactate 11.2% (SL group); an isotonic control group received 0.9% NaCl (NC group); a hypertonic control group, with the same amount of osmoles and sodium than SL group, received sodium bicarbonate 8.4% (SB group). Glomerular filtration rate (GFR) markers, coagulation and inflammation parameters were measured over a 5-h period. Immediately after euthanasia, kidneys were withdrawn for histological study. Statistical analysis was performed with nonparametric tests and the Dunn correction for multiple comparisons. A p < 0.05 was considered significant.ResultsThe direct immunofluorescence study revealed that the percentage of capillary sections thrombosed in glomerulus were significantly lesser in SL group [5 (0–28) %] compared to NC [64 (43–79) %, p = 0.01] and SB [64 (43–79), p = 0.03] groups. Alterations in platelet count and fibrinogen level occurred earlier and were significantly more pronounced in both control groups compared to SL group (p < 0.05 at 210 and 300 min). The increase in thrombin–antithrombin complexes was significantly higher in NC [754 (367–945) μg/mL; p = 0.03] and SB [463 (249–592) μg/mL; p = 0.03] groups than in SL group [176 (37–265) μg/mL]. At the end of the experiment, creatinine clearance was significantly higher in SL group [55.46 (30.07–67.85) mL/min] compared to NC group [1.52 (0.17–27.67) mL/min, p = 0.03].ConclusionsIn this study, we report that sodium lactate improves DIC-associated renal microvascular thrombosis and preserves GFR. These findings could at least partly explain the better fluid balance observed with sodium lactate infusion.

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Section: Discussionmentioning

confidence: 99%

Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study

Duburcq

Durand

Tournoys

et al. 2018

Ann. Intensive Care

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“…Another investigation showed an increase in inflammatory markers and histological lesion scores when the infusion of hypertonic saline was made 90 minutes after the induction of endotoxemia, when compared to that performed 15 minutes later 15 , including levels of IL-10. In our study, the levels of IL-10 and INF-gamma showed no significance between therapeutic groups, with all of them being significant lower than IO group, perhaps again influenced by the time of resuscitation and sampling.…”

Section: Discussionmentioning

confidence: 99%

Effects of N-acetylcysteine on the inflammatory response and bacterial translocation in a model of intestinal obstruction and ischemia in rats

et al. 2022

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Purpose: To evaluate effect of N-acetylcysteine (NAC) associated with Ringer lactate or hypertonic saline in inflammation and bacterial translocation on experimental intestinal obstruction (IO). Methods: Wistar rats was subjected to IO. Six or 24 hours after, rats were subjected to enterectomy and fluid resuscitation: IO, RL (subjected to the same procedures but with fluid resuscitation using Ringer’s lactate solution); RLNAC (added NAC to Ringer’s solution); and HSNAC (surgical procedure + fluid reposition with 7.5% hypertonic saline and NAC). After 24 h, tissues were collected to cytokines, bacterial translocation, and histological assessments. Results: In kidney, interleukin-1beta (IL-1beta) was lower in the groups with fluid resuscitation compared to IO group. The RLNAC showed lower levels compared to the RL. Interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-alpha), and ( IFN-gamma ) were lower in the treatment groups than in IO. In lung, IL-1beta and IL-6 were lower in RLNAC compared to IO. IL-10 was lower in RL, RLNAC and HSNAC compared to IO. TNF-alpha was higher in HSNAC compared to both RL and RLNAC. Bacterial translocation was observed in all animals of IO group. In kidneys, inflammation and congestion degrees were lower in HSNAC compared to RL. In lungs, inflammation levels were higher in RLNAC compared with the sham group. Conclusions: The data indicates that NAC associated with RL can promote a decrease in the inflammatory process in the kidneys and lungs in rats, following intestinal obstruction and ischemia in rats.

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“…Studies with hypertonic sodium chloride solution revealed a prompt blood pressure restoration in experimental severe hemorrhagic shock [8–12]. Hypertonic solution treatment was effective in reducing mortality rate of endotoxemic rats [13], as well as prevented lung injury by LPS in the experimental model of ARDS [14, 15]. Hypertonic solution has been used in sepsis’ experimental studies and in small clinical studies [9, 16, 17].…”

Section: Introductionmentioning

confidence: 99%

Hypertonic solution-induced preconditioning reduces inflammation and mortality rate

et al. 2019

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Background Dysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect from a lethal dose of LPS and modulates systemic inflammatory profile in mice. Methods Male Balb/C mice received intravenous (i.v.) injections of Hypertonic solution (NaCl 7.5%) (0.8 ml) for 3 days, on day 8th was challenged with LPS 15 mg/kg. Controls with Saline 0.9%, urea and sorbitol were performed. Microarray of mRNA expression was analyzed from HS versus saline from macrophages to identified the pathways activated by HS. Results HS preconditioning reduced mortality after LPS injection as well reduced the cytokines release in plasma of the animals challenged by LPS. In order to check how HS induces a preconditioning state we measured plasma cytokines after each HS infusion. Repeated HS injections induced a state of preconditioning that reprograms the inflammatory response, resulting in reduced inflammatory cytokine production. A microarray of mRNA demonstrated that Hypertonic solution increased the expression of several genes in special Mapkbp1 and Atf3. Conclusion hypertonic solution induces preconditioning/tolerance reducing mortality and inflammatory response after LPS challenge.

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Impact of Time on Fluid Resuscitation with Hypertonic Saline (NaCl 7.5%) in Rats with LPS-Induced Acute Lung Injury

Cited by 8 publications

References 25 publications

Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study

Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study

Effects of N-acetylcysteine on the inflammatory response and bacterial translocation in a model of intestinal obstruction and ischemia in rats

Hypertonic solution-induced preconditioning reduces inflammation and mortality rate

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