Impact of thymoquinone supplementation on immobilisation stress-induced changes in reproductive characteristics of male mice

Abstract

The aim of the current study was to investigate changes in the reproductive parameters during stress and the impact of thymoquinone during the period. The effects of stress were measured through immobilisation stress on mice. Group I was administered normal saline daily via intraperitoneal injection while Groups II and III were subjected to 2 and 6 hours of immobilisation stress respectively. Groups IV and V were subjected to stress for 2 and 6 hours respectively followed by intraperitoneal injection of 10 mg/kg thymoquinone which was continued on alternate days. The level of significance was set at p<0.05 and statistical analysis showed significant difference in testicular weight of mice in groups II and III compared to the controls but no significant difference was obtained for sperm count between all groups. Sperm motility, however, was significantly different among the groups under stress for 2 and 6 hours and that of 6 hours with the treatment of thymoquinone when compared to the controls. The histology of the testes also indicated a few alterations in comparison to the controls in the germinal epithelium and spermatogenic pattern in groups III and V.

Genetic, biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats

“…The results of the study by Soleimani Mehranjani et al showed that the simultaneous consumption of black seed oil and bisphenol prevents the adverse effects of bisphenol on testicular tissue of adult mice (Sh, 2015). The results of Rahman et al`s study showed that the effects of THY on stressinduced changes in the reproductive system represented that in the THY groups, the sperm motility, sperm count and testicular weight were increased significantly compared with the stressed groups (Rahman et al, 2014). In a study by Dirican et al, anti-inflammatory and anti-cancer effects of THY were approved.…”