“…Time and hand coverage were assessed in addition to bacterial load outcome in two 28,30 and three 30,32,35 studies, respectively. The participants were doctors and nurses, 28,30,35 medical students, [31][32][33] or HCWs, 6,29,34 while the settings included hospitals, 6,28,30,34,35 hospital laboratories 29,33 or university hospital training facilities. 31,32 The studies that compared the 6-step technique to other approaches [28][29][30][31]33,34 and the studies that investigated the effectiveness of the 6-step technique were analyzed and discussed separately.…”