Impact of the molecular weight on the size of chitosan nanoparticles: characterization and its solid-state application

Villegas‐Peralta, Yedidia; López‐Cervantes, Jaime; Madera-Santana, Tomás J.; Sánchez-Duarte, Reyna G.; Sánchez‐Machado, Dalia I.; Martinez‐Macias, María del Rosario; Correa-Murrieta, Ma. A.

doi:10.1007/s00289-020-03139-x

Cited by 34 publications

(20 citation statements)

References 62 publications

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“…Though nanoparticle size is inversely related to the chitosan concentration used in their fabrication, a situation where more free NH + 3 groups are available to react with TPP and having a chitosan concentration beyond 0.3% w/v are reported to increase the nanoparticle size [49,59,60]. Our findings were contradictory to this, where with the use of 0.4% w/v chitosan concentration, a slight decrease in the nanoparticle size (Student's t-test, p = 0.260) was observed which can be attributed to the chitosan of low molecular weight used in the nanoparticle fabrication [61] (Table 1). Similarly, drug loading is also expected to further increase nanoparticles size, being an additional formulation moiety which is either adsorbed at the surface or entrapped in the nanoparticle core, and hence the particle size of the drug loaded nanoparticles was found to be significantly increased (Student's t-test, p = 0.015) compared to blank nanoparticles [49,62,63] as shown in Table 1.…”

Section: Discussioncontrasting

confidence: 85%

Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Basit

Amin

et al. 2020

Polymers

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Improved physicochemical properties of chitosan-curcumin nanoparticulate carriers using microwave technology for skin burn wound application are reported. The microwave modified low molecular weight chitosan variant was used for nanoparticle formulation by ionic gelation method nanoparticles analyzed for their physicochemical properties. The antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa cultures, cytotoxicity and cell migration using human dermal fibroblasts—an adult cell line—were studied. The microwave modified chitosan variant had significantly reduced molecular weight, increased degree of deacetylation and decreased specific viscosity. The nanoparticles were nano-sized with high positive charge and good dispersibility with entrapment efficiency and drug content in between 99% and 100%, demonstrating almost no drug loss. Drug release was found to be sustained following Fickian the diffusion mechanism for drug release with higher cumulative drug release observed for formulation (F)2. The microwave treatment does not render a destructive effect on the chitosan molecule with the drug embedded in the core of nanoparticles. The optimized formulation precluded selected bacterial strain colonization, exerted no cytotoxic effect, and promoted cell migration within 24 h post application in comparison to blank and/or control application. Microwave modified low molecular weight chitosan-curcumin nanoparticles hold potential in delivery of curcumin into the skin to effectively treat skin manifestations.

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Section: Discussioncontrasting

confidence: 85%

Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Basit

Amin

et al. 2020

Polymers

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“…Even though CHS has a semi crystalline structure, this behavior reduces because of the breaking of hydrogen bonds when it is crosslinked with STPP (Fan, Yi, Zhang, & Yokoyama, 2017). These results correspond well with the XRD patterns reported by Villegas-Peralta et al (Villegas-Peralta et al, 2020).…”

Section: Ftir-atr Analysissupporting

confidence: 91%

“…The lowest zeta potential value was obtained for HCHS-NPs, which due to agglomerates. In a similar study, Villegas-Peralta et al (Villegas-Peralta et al, 2020) reported that when molecular weight in CHS increases, a reduction in the zeta potential was observed.…”

Section: Hydrodynamic Diameter and Zeta Potential Measurementmentioning

confidence: 73%

Preparation of Allantoin Loaded Chitosan Nanoparticles and Influence of Molecular Weight of Chitosan on Drug Release

Ulu

2020

İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi

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In this study, allantoin-loaded chitosan nanoparticles were prepared and the effect of molecular weights (low, medium, and high) of chitosan was investigated on the release of allantoin. The allantoin-loaded chitosan nanoparticles were characterized by various methods. The morphology, size, zeta potential, and loading efficiency were affected by the molecular weight of chitosan. The results displayed that the average hydrodynamic diameter of chitosan nanoparticles was in the range of 427-768 nm and the zeta potential values from 5.97 to 5.12 mV was obtained. The allantoin release from chitosan nanoparticles reached the plateau level in 96 hours. Cumulative allantoin release values of low, medium, and high molecular mass chitosan nanoparticles were determined as 43%, 30%, and 27%, respectively. According to the results, the molecular weight of chitosan influenced on allantoin release.

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“…The TPP will create strong binding flex with the amine molecules of chitosan to stabilize the matrix. In addition, TPP is less toxic compared to any other chemical crosslinker, such as glutaraldehyde [28,35,36]. In this study, we aimed to formulate the nanochitosan by using the medium molecular weight chitosan under an acidic environment.…”

Section: E2f1 Mrna Expression Analysis After Ch-np-antimir-106b-5p Trmentioning

confidence: 99%

“…Molecular weight may affect the transfection ability and medium weight has notable advantages to induce better bioavailability since it carries more amines and hydroxyl molecules inside. The acidity of the dissolving solution also enhances the protonation among the amine molecules [36,37].…”

Section: E2f1 Mrna Expression Analysis After Ch-np-antimir-106b-5p Trmentioning

confidence: 99%

Chitosan Nanoparticles Mediated Delivery of Mir-106b-5p to Breast Cancer Cell Lines McF-7 and T47d

et al. 2021

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Objective: The development of nanomedicine, such as miRNA transfection to cancer cells,has widely gained interest in the past decade. Unfortunately, miRNA tends to decay easily by the cellular enzymatic process and requires a carrier. As a cationic biopolymer, chitosan is widely known as a non-viral vector. However, research about chitosan as a miRNA delivery system remains limited. This study aimed to investigate the effect and characters of synthetic miRNA loaded chitosan nanoparticles on breast cancer cell lines. Methods: To obtain the nanocomplex, chitosan-antimiR-106b-5p was formulated using natriumtripolyphosphate through ionic gelation methods. The nanochitosan formula was characterized by using gel electrophoresis; Nano Quant for encapsulation of entrapment quantification; morphology appearance as viewed by Scanning Electron Microscope (SEM), nanochitosan size analysis; in vitro analysis using MCF-7 and T47D breast cancer cell lines; in silico prediction of possible gene target; polymerase chain reaction analysis and gel electrophoresis for E2F1/GAPDH expression. Results: The efficiency entrapment value was 96.7%, particle size analysis was 458±11.79 nm, and polydispersity index (PDI) was 0.65±0.07, with spherical morphology as viewed in SEM. There was no significant difference between the nanochitosan supplemented group and the control group in MCF-7 cells (p=0.067). However, the ratio of E2F1 to GAPDH was significantly lower than the control group after nanochitosan antimiR-106b-5p was loaded at concentration 140 nmol (p=0.022) and 35 nmol (p=0.016). Conclusion: Our nanochitosan formula is non-toxic to use in MCF-7 cell lines. Most importantly, as the formula was conjugated to synthetic antimiR-106b-5p, the E2F1 expression decreased.

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Impact of the molecular weight on the size of chitosan nanoparticles: characterization and its solid-state application

Cited by 34 publications

References 62 publications

Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Preparation of Allantoin Loaded Chitosan Nanoparticles and Influence of Molecular Weight of Chitosan on Drug Release

Chitosan Nanoparticles Mediated Delivery of Mir-106b-5p to Breast Cancer Cell Lines McF-7 and T47d

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