Impact of the lipid bilayer on energy transfer kinetics in the photosynthetic protein LH2

Ogren, John I.; Tong, Ashley L.; Gordon, Samuel C.; Chenu, Aurélia; Lü, Yue; Blankenship, Robert E.; Cao, Jianshu; Schlau‐Cohen, Gabriela S.

doi:10.1039/c7sc04814a

Cited by 22 publications

(33 citation statements)

References 93 publications

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“…3A-B). Such a reorientation of the B800 rings has been implicated in the enhancement of the energy transfer rate between the B800 and B850 rings (Ogren et al, 2018). The QM/MM computations indicate a selective broadening of the B800 absorption band, relative to B850 (Figs.…”

Section: Segregation Of Proteins Enables Heterogeneous Membrane Curvamentioning

confidence: 93%

“…Each LH2 complex is composed of a ring of nine B800 molecules oriented perpendicularly to nine B850 and nine B850 molecules. (B) Orientation of the B800 pigments relative to their crystallographic geometry (PDB ID: 1NKZ) about the transition-dipole, NB-ND axis (inset) (Ogren et al, 2018) for LH2 in flat membrane (black) vs. in the chromatophore (red). A statistical shift is observed in the chromatophore towards B800 orientation angles of 11 o relative to the crystallographic geometry.…”

Section: Segregation Of Proteins Enables Heterogeneous Membrane Curvamentioning

confidence: 99%

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Atoms to Phenotypes: Molecular Design Principles of Cellular Energy Metabolism

et al. 2019

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Bioenergetic membranes are the key cellular structures responsible for coupled energy-conversion processes, which supply ATP and important metabolites to the cell. Here, we report the first 100million atom-scale model of an entire photosynthetic organelle, a chromatophore membrane vesicle from a purple bacterium, which reveals the rate-determining steps of membrane-mediated energy conversion. Molecular dynamics simulations of this bioenergetic organelle elucidate how the network of bioenergetic proteins influences membrane curvature and demonstrates the impact of thermal disorder on photosynthetic excitation transfer. Brownian dynamics simulations of the quinone and cytochrome c2 charge carriers within the chromatophore interior probe the mechanisms of nanoscale charge transport under various pH and salinity conditions. Reproducing phenotypic properties from atomistic details, a rate-kinetic model evinces that low-light adaptations of the bacterium emerge as a spontaneous outcome of optimizing the balance between the chromatophore's structural integrity and robust energy conversion. Put together, the hybrid structure determination and systems-level modeling of the chromatophore, in conjunction with optical spectroscopy, illuminate the chemical and organizational design principles of biological membranes that foster energy storage and transduction in living cells. Parallels are drawn with the more universal mitochondrial bioenergetic machinery, from whence molecular-scale insights on the mechanism of cellular aging are inferred. This endeavor made feasible through the advent of petascale supercomputers, paves the way to first-principles modeling of whole living cells.

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Section: Segregation Of Proteins Enables Heterogeneous Membrane Curvamentioning

confidence: 93%

Section: Segregation Of Proteins Enables Heterogeneous Membrane Curvamentioning

confidence: 99%

Atoms to Phenotypes: Molecular Design Principles of Cellular Energy Metabolism

et al. 2019

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“…For example, the fluorescence lifetime of LHCII has been reported to be different in a lipid environment as compared to in detergent micelles 28,29 . The in vitro environments, which employ detergent or crystallization, may introduce additional, non-native conformational changes that could alter or even denature the functional structure of membrane proteins [30][31][32] . In contrast, in vivo spectroscopy on whole leaves provides physiological information [33][34][35] .…”

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confidence: 99%

Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

et al. 2020

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Plants prevent photodamage under high light by dissipating excess energy as heat. Conformational changes of the photosynthetic antenna complexes activate dissipation by leveraging the sensitivity of the photophysics to the protein structure. The mechanisms of dissipation remain debated, largely due to two challenges. First, because of the ultrafast timescales and large energy gaps involved, measurements lacked the temporal or spectral requirements. Second, experiments have been performed in detergent, which can induce nonnative conformations, or in vivo, where contributions from homologous antenna complexes cannot be disentangled. Here, we overcome both challenges by applying ultrabroadband twodimensional electronic spectroscopy to the principal antenna complex, LHCII, in a near-native membrane. Our data provide evidence that the membrane enhances two dissipative pathways, one of which is a previously uncharacterized chlorophyll-to-carotenoid energy transfer. Our results highlight the sensitivity of the photophysics to local environment, which may control the balance between light harvesting and dissipation in vivo.

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“…2 ). In the LH2 complexes, B800 molecules are localized near the cytoplasmic side of the chromatophore membrane and protrude from the protein (Ogren et al 2018 ). For these reasons, B800 seems most vulnerable to the effects of antiseptics.…”

Section: Discussionmentioning

confidence: 99%

The effect of some antiseptic drugs on the energy transfer in chromatophore photosynthetic membranes of purple non-sulfur bacteria Rhodobacter sphaeroides

et al. 2021

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Impact of the lipid bilayer on energy transfer kinetics in the photosynthetic protein LH2

Abstract: Embedding photosynthetic light-harvesting complexes in membrane discs enables measurements of the physiological energy transfer kinetics.

Cited by 22 publications

References 93 publications

Atoms to Phenotypes: Molecular Design Principles of Cellular Energy Metabolism

Atoms to Phenotypes: Molecular Design Principles of Cellular Energy Metabolism

Observation of dissipative chlorophyll-to-carotenoid energy transfer in light-harvesting complex II in membrane nanodiscs

The effect of some antiseptic drugs on the energy transfer in chromatophore photosynthetic membranes of purple non-sulfur bacteria Rhodobacter sphaeroides

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