Impact of the inoculum composition on the structure of the total and active community and its performance in identically operated anaerobic reactors

Culture-Independent Analysis of Linuron-Mineralizing Microbiota and Functions in on-Farm Biopurification Systems via DNA-Stable Isotope Probing: Comparison with Enrichment Culture

“…The amplicons were obtained and purified according to the Illumina “16S Metagenomic Sequencing Library Preparation” protocol (Illumina, San Diego, CA). Library preparation and sequencing on the Illumina MiSeq platform were performed as described . Sequencing analysis was performed using R version 3.6.2 with packages: BiocManager, dada2, Phyloseq, Biostrings, ggplot2 and ggsci .…”

“…Library preparation and sequencing on the Illumina MiSeq platform were performed as described. 40 Sequencing analysis was performed using R version 3.6.2 41 with packages: BiocManager, 42 dada2, 43 Phyloseq, 44 Biostrings, 45 ggplot2 46 and ggsci. 47 Quality control and sequencing analysis resulted in between 40000−200000 valid reads per sample for the SIP experiments and ∼22000 valid reads for the EC.…”

Convergent Microbial Community Formation in Replicate Anaerobic Reactors Inoculated from Different Sources and Treating Ersatz Crew Waste

“…In the current study, a marked decrease in diversity was noted in comparing the lab-scale reactors to the inocula, and this was most dramatic for the Bog-inoculated reactors. Previous studies have demonstrated a similar decrease in community diversity when comparing lab-to fullscale reactors [16,17]. Scaling down the anaerobic digestion process contributes to a loss of microbial community complexity as full-scale reactors are more likely to harbor environmental niches that can support greater microbial diversity.…”

“…Similar to the findings of the other studies, the samples in this study clustered by the reactor from which they were taken, and the replicate reactors clustered according to the inoculum source, suggesting persistent and detectable differences in the community structure. Additionally, microbial communities in the lab-scale reactors were more similar to each other than were the communities of the original inocula, suggesting some community convergence in the lab-scale reactor environment [17]. Community convergence was also detected by Peces et al, who examined the temporal community changes of CSTRs processing cellulose and casein that were inoculated from four full-scale anaerobic reactor sources [18].…”

“…However, the triplicate reactors inoculated from a single source demonstrated different performance and different microbial communities, also suggesting stochastic influences in community assembly [16]. Lemoine et al inoculated replicate batch reactors from three full-scale digesters, two granular and one CSTR, which were fed pasteurized synthetic dairy wastewater until stable operation was achieved [17]. Performance was similar for all the reactors, regardless of the inoculum.…”