After the introduction of the phlebotomist team, there was a reduction of samples discarded because of incorrect technique or route of collection, from 54 to only 4 after the intervention (P < .001). When evaluating contamination, 98 contaminated blood cultures were recorded before the intervention, establishing a baseline rate of 12.48% (98/785). With the work of technicians, the number of contaminated blood cultures was 67, leading to a rate of 7.9% (67/848). The intervention with the phlebotomist team significantly reduced the contamination rate (P = .002). Regarding culture site, 2 blood cultures in the postintervention period had no indication of the collection site and were not accounted for in the following analysis. Throughout the 13-month period analyzed in the study, 58.4% (953/1,633) of the blood cultures were collected in intensive care units and corresponded to 74.8% (122/163) of all contaminations. When comparing the respective contamination rates of each site, the reduction in the overall contamination rate was because of a significant reduction in the contamination of samples collected in intensive care units, which were reduced from 17.24% (75/435) to 9.07% (47/518) and presented statistical significance (P < .001). Single blood cultures corresponded to 5.73% of the total valid samples (205/ 3,579), and there was no significant change in their total amount between the analyzed periods (P = .144). Although they were disregarded for the contamination rate calculation, a reduction was observed in samples classified as possibly contaminated, which went from 17 to 4 (P = .005). The use of a phlebotomy team, associated with an educational intervention, can significantly reduce the blood culture contamination rate, especially in sites with a history of high contamination, such as intensive care units.