Impact of the Distinct Sampling Area on Volumetric Features of Gingival Crevicular Fluid

Hatipoğlu, Hasan; Yamalik, Nermin; Berberoğlu, Atilla; Eratalay, Kenan

doi:10.1902/jop.2007.060331

Cited by 28 publications

(18 citation statements)

References 45 publications

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“…Uttio 16) reported that in periodontitis, increased in GCF volume was associated with increased in probing pocket depth and deeper pockets were associated with higher GCF volume. Finding in the present study supports the suggestion that GCF volume increases with gingival inflammation and periodontitis 14,18,38,39) . In the present study, when GCF volume was compared at healthy gingival sites in all 3 groups, there was significant increase in GCF volume in both gingivitis and periodontitis group compared to healthy gingival group.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of the Impact of the Clinical Periodontal Status on Volumetric Features of Gingival Crevicular Fluid by using Periotron<sup>®</sup> 8000

Sehdev

Ganji

Bhongade

et al. 2017

J. Hard Tissue Biology.

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Clinical periodontal status is among the major determinants of the volumetric features of GCF. Previous studies have shown that probing depth, presence/severity of gingival and periodontal inflammation affect the biodynamics of GCF. The aim of the present study was to evaluate the impact of the clinical periodontal status on volumetric features of gingival crevicular fluid. Thirty-six patients were equally divided into healthy group, gingivitis group and periodontitis group. Clinical periodontal status was assessed by recording: papillary bleeding index, probing pocket depth and clinical attachment level. Gingival crevicular fluid (GCF) samples were obtained from 4 sites in each patient. The mean volume of GCF was determined in each group for comparison. Further in periodontitis group, GCF samples were also collected from healthy sites, gingivitis sites and periodontitis sites. Mean GCF volume recorded by using Periotron® 8000 in healthy gingival group, gingivitis group and periodontitis group were: 0.04, 0.09, 0.78 µl respectively. There was increase in GCF volume in a diseased related pattern, healthy gingival group < gingivitis group < periodontitis group. In periodontitis group, the sites with healthy gingiva showed significantly higher GCF volume than healthy gingival group (0.11µl Vs 0.04µl), similarly, the sites with gingivitis in periodontitis group also showed significantly higher GCF volume than gingivitis group (0.32µl Vs 0.11µl). The present study suggests that the volumetric analysis of GCF could be sensitive and reliable indicator of periodontal health. The ability of GCF volume as a chair side measure to differentiate healthy sites from sites with mild disease within the same mouth is considered noteworthy.

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Section: Discussionsupporting

confidence: 92%

Evaluation of the Impact of the Clinical Periodontal Status on Volumetric Features of Gingival Crevicular Fluid by using Periotron<sup>®</sup> 8000

Sehdev

Ganji

Bhongade

et al. 2017

J. Hard Tissue Biology.

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“…The wide range of volumetric distribution, the site‐specific nature, and the impact of a distinct sampling site on the volume were described as important features of GCF. A standardization of the extent of probing depth, degree of gingival inflammation, and distinct sampling may improve the reliability of GCF methology 29 . For this reason, only patients with comparable probing depths, good oral hygiene (plaque index <0.35%), 18 and low gingival inflammation (sample collection after the hygiene phase) to avoid contamination of GCF samples with blood were chosen.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Gingival Crevicular Fluid Sampling Methods in Patients With Severe Chronic Periodontitis

Guentsch

Kramesberger²,

Sroka

et al. 2011

Journal of Periodontology

112

106

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Background Analysis of samplings from periodontal pockets is important in diagnosis and therapy control of periodontitis. In this study, three different sampling techniques were compared to determine if one method can yield samples suitable for reproducible and simultaneous determination of bacterial load, cytokines, neutrophil elastase, and Arg-specific gingipains. R-gingipains are an important virulence factor of Porphyromonas gingivalis, the exact concentration of which in gingival crevicular fluid (GCF) has not yet been quantified. Methods GCF was sampled from four sites per patient (each two sites one method) in 36 chronic periodontitis patients. One week later, the procedure was repeated with alternative methods. The variables that had been determined were: loads of Aggregatibacter actinomycetemcomitans and P. gingivalis, levels of interleukin-6 and interleukin-8, activity of neutrophil elastase and level of R-gingipains. Results The detected cytokine levels were higher using paper strips compared to paper points. Bacteria were found in similar loads from the paper strips and paper points. R-gingipains were detectable in high quantities only by washing of the periodontal pocket. The level of R-gingipains correlated with the load of P. gingivalis. Conclusion The use of paper strips is suitable for simultaneous determination of microbial and immunological parameters. Obtaining GCF by washing can be useful for special purposes. Gingipain concentration in periodontal pockets was directly determined to be up to 1.5 μM. This value indicates that most of so far identified substrates of these proteases by in vitro assays can be easily degraded in P. gingivalis infected sites.

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“…Another study [21] demonstrated that very little concentrations of IL-12 in GCF were detected, with the levels decreasing with increasing inflammation. It is well established that with increasing inflammation, there are increasing amounts of GCF [22,23]. This could partly be the reason for the higher concentrations of IL-12 in healthy sites in the previous studies.…”

Section: Discussionmentioning

confidence: 73%

Effect of Periodontal Therapy on Salivary Interleukin-12 Levels in Chronic Periodontitis

Sharma

Khattak

Naagtilak

et al. 2014

JCDR

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Impact of the Distinct Sampling Area on Volumetric Features of Gingival Crevicular Fluid

Cited by 28 publications

References 45 publications

Evaluation of the Impact of the Clinical Periodontal Status on Volumetric Features of Gingival Crevicular Fluid by using Periotron<sup>®</sup> 8000

Evaluation of the Impact of the Clinical Periodontal Status on Volumetric Features of Gingival Crevicular Fluid by using Periotron<sup>®</sup> 8000

Comparison of Gingival Crevicular Fluid Sampling Methods in Patients With Severe Chronic Periodontitis

Effect of Periodontal Therapy on Salivary Interleukin-12 Levels in Chronic Periodontitis

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