Impact of the blue-carba rapid test for carbapenemase detection on turnaround time for an early therapy against Pseudomonas aeruginosa

Cunha, Rick Shandler Rodrigues da; Carniel, Eliana; Narváez, Gabriel; Dias, Cícero; Perez, Leandro Reus Rodrigues

doi:10.1016/j.ajic.2020.08.018

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…The predominance of MBL was also reported by Bitar et al [28]. O'Neall et al reported that VIM was the most predominant (20.8 %) in their isolates while NDM was detected only in 2.8 % [23].…”

Section: Novaissupporting

confidence: 70%

“…Carbapenemase production can be detected in the laboratory by many phenotypic and genotypic methods. Among the phenotypic methods the Carba Blue test has been evaluated and found to be both rapid, accurate and cost-effective [23]. However, the test may give false negative result with week or lowlevel carbapenemase production, especially with metallo-beta lactamases (MBL) [24].…”

Section: Detection Of Carbapenemasesmentioning

confidence: 99%

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Susceptibility of multi-drug resistant isolates of Pseudomonas aeruginosa to ceftazidime/aviabactam and ceftolozane/tazobactam in Benha University Hospital in Egypt and detection of carbapenemase genes

Fayed,

Mohammed,

Khashaba

et al. 2023

Microbes and Infectious Diseases

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Background: Multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) is a major public health threat. Ceftazidime/avibactam (CZA) and ceftolozane/tazobactam (C/T) are active against many MDR P. aeruginosa, and can improve the management of infected patients. However, resistance to the new antimicrobials has emerged in many countries. In this study we aimed to test the susceptibility of clinical isolates of MDR P. aeruginosa in our hospital to CZA and C/T, and to identify the role of carbapenemases in antimicrobial resistance. Methods: We studied 51 MDR P. aeruginosa isolates from 51 ICU patients admitted at Benha University Hospital in Egypt. Identification was done by VITEK-2 (BioMerieux). Testing the antimicrobial susceptibility was performed by disk diffusion method according to CLSI 2022. For all isolates, we used Carba Blue Test for phenotypic identification of carbapenemases, and multiplex PCR for detection of carbapenemase genes. Results: The isolates were 98% resistant to meropenem, while 23.5% were susceptible to each of C/T and CZA. Carbapenemase production was common, mostly by metallo-beta-lactamases (MBL). The most common carbapenemase genes were NDM, VIM and SIM. Carba Blue test identified carbapenemases in 52.9%, while multiplex PCR identified carbapenemase genes in 91.1%. Conclusion: Compared to conventional antibiotics, both CZA and C/T have shown moderate efficacy against our MDR P. aeruginosa isolates, making them good choices for the treatment of infection by some MDR P. aeruginosa. PCR is more sensitive than Carba Blue in detection of carbapenemase production.

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Section: Novaissupporting

confidence: 70%

Section: Detection Of Carbapenemasesmentioning

confidence: 99%

Susceptibility of multi-drug resistant isolates of Pseudomonas aeruginosa to ceftazidime/aviabactam and ceftolozane/tazobactam in Benha University Hospital in Egypt and detection of carbapenemase genes

Fayed,

Mohammed,

Khashaba

et al. 2023

Microbes and Infectious Diseases

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“…5,6 Concordantly, we have shown the importance of an early BCT result when applied in infections caused by Pseudomonas aeruginosa isolates. 7 For the infection control point of view, in our study, for 25 patients (16%) and 121 patients (78%) anticipated the installation of standard and contact precaution, respectively, based on an early BCT notification. On the other hand, from a clinical point of view, this fact means that an active antimicrobial agent (mostly polymyxin B in our study) should be initiated or included for adequacy of therapy.…”

mentioning

confidence: 73%

Impact of early carbapenemase notification on infection control management and antimicrobial stewardship

Perez

Narváez

Dias

2021

Infect. Control Hosp. Epidemiol.

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issues including governance and ownership, for which interdepartmental communication was efficient within the UHS. Specifically, the tracking system expanded the healthcare informatics system that pharmacists were familiar with, and its user-friendly interfaces for PPE providers and consumers helped expedite distribution processes. 5 The UHS and the TCDC have also promoted the system to increase the distribution channels, within which government offices may also allot masks to lessen the burden on healthcare providers.Because masks alone are not effective without combining infection-control measures, 7 we recommend this integrative platform for the maintenance of more PPE stockpiles, including critical infection-control equipment to reduce iatrogenic SARS-CoV-2 exposure.

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“…The methodological quality of six diagnostic accuracy studies was assessed by the QUADAS-2 guideline. Five studies were of high quality with scores ranging from 13 to 17 points [ 24 – 28 ], while one study was of medium quality (11 points) [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

Shahab,

van Veen,

Büchler

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Detection of carbapenem-resistant Pseudomonas aeruginosa (CR-PA) in humans is important to prevent transmission. However, the most optimal culture method to detect CR-PA is unknown. This systematic review aims to determine which culture method is most sensitive and which culture methods are used to detect CR-PA in humans. Second, to establish the most feasible culture method taking into account the turnaround time (TAT), and third, to provide an overview of the sampling sites used to detect carriage. Methods We systematically searched the electronic databases Embase, Medline Ovid, Cochrane, Scopus, CINAHL, and Web of Science until January 27, 2023. All diagnostic accuracy studies comparing two or more culture methods to detect CR-PA and recent outbreak or surveillance reports on CR-PA carriage or infection in humans, which describe culture methods and their results, were eligible for inclusion. We used QUADAS-2 guideline for diagnostic accuracy studies and the STROBE or ORION guideline for outbreak-surveillance studies to assess the risk of bias. Results Six diagnostic accuracy studies were included. An enrichment broth was found to increase the detection of CR-PA. Using an enrichment broth extended the TAT by 18–24 h, yet selective media could reduce the TAT by 24 h compared to routine media. In total, 124 outbreak-surveillance studies were included, of which 17 studies with surveillance samples and 116 studies with clinical samples. In outbreak-surveillance studies with surveillance samples, perianal, rectal swabs or stools were the most common sampling site/specimen (13/17, 76%). A large variety was observed in whether and which kind of enrichment broth and selective media were used. Conclusions We found a benefit of using an enrichment step prior to inoculation of the material onto selective media for the detection of CR-PA. More research is needed to determine the most sensitive sampling site and culture method. Trail registration: This study was registered in the PROSPERO International prospective register of systematic reviews (registration number: CRD42020207390, http://www.crd.york.ac.uk/PROSPERO/display_record.asp?ID=CRD42020207390).

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Impact of the blue-carba rapid test for carbapenemase detection on turnaround time for an early therapy against Pseudomonas aeruginosa

Cited by 5 publications

References 8 publications

Susceptibility of multi-drug resistant isolates of Pseudomonas aeruginosa to ceftazidime/aviabactam and ceftolozane/tazobactam in Benha University Hospital in Egypt and detection of carbapenemase genes

Susceptibility of multi-drug resistant isolates of Pseudomonas aeruginosa to ceftazidime/aviabactam and ceftolozane/tazobactam in Benha University Hospital in Egypt and detection of carbapenemase genes

Impact of early carbapenemase notification on infection control management and antimicrobial stewardship

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

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