Very few studies are available on workable air pollution treatment, especially for airborne microbiota on human health. The present study has attempted to summarize the commonly known forms of air pollution, airborne viral, bacterial, and fungal attack, their possible effects on human health, and possible treatment. A testing rig is also designed and developed for air purification. Earlier, air purifiers were used to deliver clean air by treating particulate matters (PM) only, but due to the pandemic situation, i.e., COVID-19, air purifier units are expected to treat potentially harmful microbiota as well. In the present study, HEPA (High-Efficiency Particulate Air) filter is studied in combination with conventional mechanical filters. It is observed to be very efficient in trapping and neutralizing viruses and bacteria when used with sterilization techniques. This article also highlights the critical aspects required to be examined for the installation of air purification unit.