Impact of temperature on the survival and the biocontrol efficacy of Lysobacter capsici AZ78 against Phytophthora infestans

Abstract

Temperature can strongly influence the biocontrol efficacy of Lysobacter strains when used against phytopathogens. We investigated the influence of temperature on physiological characteristics of Lysobacter capsici AZ78 in a biocontrol situation. L. capsici AZ78 effectively colonized tomato leaves at 15 and 25°C while exposure to 35 and 37°C was highly detrimental. Accordingly, Phytophthora infestans attacks on tomato leaves were controlled when L. capsici AZ78 was pre-exposed to 15 and 25°C for 24 h. Conversely, exposure to 35°C drastically reduced the biocontrol efficacy of L. capsici AZ78 probably by impairing anti-oomycete and proteolytic activity. Furthermore, the exposure to 15°C made the L. capsici AZ78 cells more resistant to UV light and copper ions compared to 25 and 35°C. These findings give an indication on the best temperature range for the future use of biofungicides based on L. capsici AZ78 cells.

Probiotic Consortia: Reshaping the Rhizospheric Microbiome and Its Role in Suppressing Root-Rot Disease of Panax notoginseng

“…L. antibioticus produces lytic enzymes, a toxic compound that inhibits the growth of Phytophthora capsici and also restrains the DNA synthesis of the pathogen by producing a chemical named Myxin (Ko et al, 2009;Chowdhury et al, 2011). The growth of phytopathogenic Oomycetes (Plasmopara viticola and Phytophthora infestans) can be restricted by the secondary metabolites produced by Lysobacter capsici AZ78 (Puopolo et al, 2014(Puopolo et al, , 2015. Pseudomonas spp.…”

The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors

“…In comparison to L. enzymogenes , much less is known about the biological features of L. capsici (Puopolo et al, 2015 ). As understanding the biological characteristics of a microorganism is crucial for its development as a biopesticide, we sequenced the Lc AZ78 genome using PacBio technology and carried out functional experiments to assess the biological properties predicted by the genome analysis.…”

Stepwise flow diagram for the development of formulations of non spore-forming bacteria against foliar pathogens: The case of Lysobacter capsici AZ78

“…AZ78 alone had no efficacy in the field trials, and this may be explained by its susceptibility to environmental factors such as temperature (Puopolo et al 2015). Indeed, temperature values below the optimal temperature for AZ78 efficacy (25°C; Puopolo et al 2015) occurred during the 24 h in which the experiments took place. Moreover, other environmental conditions such as UV light and desiccation negatively affected the persistence of AZ78 cells on grapevine leaves.…”