Impact of Temperature, Nutrients and Heavy Metals on Bacterial Diversity and Ecosystem Functioning Studied by Freshwater Microcosms and High-Throughput DNA Sequencing

Impact of heavy metal residues on the diversity of bacteria isolated from a few water points in areas of agricultural activity

“…These studies, mostly conducted in large cities, took into account the effect of pollution by multiple heavy metals on bacterial diversity and community structure in agricultural soils [8]. Other work focuses on the impact of temperature, nutrients and heavy metals on bacterial diversity and the functioning of ecosystems studied by freshwater microcosms and high-throughput DNA sequencing [9] Despite these data, little information is available on the levels of heavy metals present in water intended for human consumption in rural agricultural areas. Moreover, little is known about the impact that these heavy metals would have on the variation in the diversity of bacteria present in drinking water.…”

Investigation of the microbial community structure and diversity in the environment surrounding a veterinary antibiotic production factory

“…The second-generation of sequencing technology emerged in 2005, which can read millions of reads typically restricted to 100-500 nucleotide regions, such as single variable regions and combinations of variable regions of the bacterial 16S rRNA gene. [28][29][30] However, the short read approach introduces biases and can't provide effective resolution below the bacterial genus level. Until recently, an alternative for full-length bacterial 16S rRNA gene sequencing is provided by the third-generation single-molecule real-time sequencing technology.…”