Impact of Targeted Specific Antibiotic Delivery for Gut Microbiota Modulation on High-Fructose-Fed Rats

Abstract

The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fecal microbiota community, oral glucose tolerance, the markers of liver injury, plasma and hepatic lipids profile, and histological evaluation were investigated. The levels of blood glucose, liver injury markers, lipid profile in plasma and liver, and fat tissue were significantly increased in high-fructose-fed rats. However, after pH-sensitive cefdinir microsphere administration, the elevation of these parameters was significantly suppressed. Cef EL significantly lowered the increased AST (p < 0.05) and ALT (p < 0.001) levels in HFD group. There is a significant lower (p < 0.01) AUCglucose level in Cef EL group than HFD group The histological changes in the liver and the small and large intestines were more profound in HFD group as compared to cefdinir-treated HFD and control groups. Feeding of cefdinir microsphere sustained lactobacilli and bifidobacteria and significantly decreased (p < 0.05) the number of Enterobacteriaceae induced by HFD. Experimental evidences demonstrated that the effectiveness of pH-specific cefdinir microsphere on reducing insulin resistance and development of metabolic changes in high-fructose-fed rats and suggested that it may be a promising therapeutic agent in treating type 2 diabetes. Intestinal-targeted antibiotic delivery needs to be further explored for its therapeutic applications.

Deleterious Metabolic Effects of High Fructose Intake: The Preventive Effect of Lactobacillus kefiri Administration

“…in FRD-mice, independently if they received or not L. kefiri . This result agrees with those previously shown by Di Luccia et al and Jena et al , who reported a decrease in Lactobacillus in high fructose-fed rats [67,68]. When we evaluated the effect of L. kefiri administration we observed that it has a significant positive influence in both Firmicutes and Bacteroidetes phyla, and also in two of the four specific populations studied, B. fragilis and L. murinus .…”

Dietary Carbohydrate Constituents Related to Gut Dysbiosis and Health

“…Many studies reported that a high carbohydrate diet changes the intestinal microbiota, which is associated with metabolic diseases such as type 2 diabetes, cardiovascular disease, and IBD [29,71]. High sugar consumptions induced obesity, insulin resistance, inflammation, and metabolic dysfunction due to changes in the intestinal microbiota in animal studies [56,93]. There are still few studies on the correlation between microbiota changes due to carbohydrate diets and metabolic disorders in human trials, but it is now becoming increasingly clear with recent studies.…”

Obesity and Clinical Riskiness Relationship: Therapeutic Management by Dietary Antioxidant Supplementation—a Review

“…A significant increase in total refined carbohydrate intake and fructose has paralleled recent increase in incidence of obesity and diabetes [17]. Metabolism of sugars, particularly fructose, occurs mainly in the liver, and high-fructose flux leads to enhanced hepatic triglyceride accumulation resulting in impaired glucose and lipid metabolism and increased pro-inflammatory cytokine expression [18].…”