Biochemical Pharmacology volume 80, issue 6, P811-818 2010 DOI: 10.1016/j.bcp.2010.05.021 View full text
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Xuetao Fan, Douglas D. Ross, Hiroshi Arakawa, Vadivel Ganapathy, Ikumi Tamai, Takeo Nakanishi

Abstract: Amino acids activate nutrient signaling via the mammalian target of rapamycin (mTOR), we therefore evaluated the relationship between amino acid transporter gene expression and proliferation in human ovarian cancer cell lines. Expression of three cancer-associated amino acid transporter genes, LAT1, ASCT2 and SN2, was measured by qRT-PCR and Western blot. The effects of silencing the LAT1 gene and its inhibitor BCH on cell growth were evaluated by means of cell proliferation and colony formation assays. The sy…

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