Impact of surface topography on biofilm formation by Candida albicans

Abstract

Candida albicans is a fungal pathogen that causes serious biofilm-based infections. Here we have asked whether surface topography may affect C. albicans biofilm formation. We tested biofilm growth of the prototypical wild-type strain SC5314 on a series of polydimethylsiloxane (PDMS) solids. The surfaces were prepared with monolayer coatings of monodisperse spherical silica particles that were fused together into a film using silica menisci. The surface topography was varied by varying the diameter of the silica particles that were used to form the film. Biofilm formation was observed to be a strong function of particle size. In the particle size range 4.0–8.0 μm, there was much more biofilm than in the size range 0.5–2.0 μm. The behavior of a clinical isolate from a clade separate from SC5314, strain p76067, showed results similar to that of SC5314. Our results suggest that topographic coatings may be a promising approach to reduce C. albicans biofilm infections.

Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity ofCandida albicansand Inflammatory Host ResponseIn Vitro

“…In this study, we used C. albicans SC5314 strain, and the MIC was found in the range of 6.25–12.5 µ M. C. albicans 5314 was used because the genome is fully described, with well-known molecular patterns and phenotypes. In addition, the biofilm formation by this strain is well established in several studies [ 22 , 23 ].…”

Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

“…For Amp, samples were taken every 30 min during the first 7 h of incubation and then at 24 and 48 h after inoculation. For Ofx, samples were taken at every 30 min during the first 7 h and then at 10,13,16,19,20,22, and 24 h. For the Amp and Tob treatment during the early-stage biofilm formation of uropathogenic E. coli ATCC 53505 and P. aeruginosa PAO1, samples were taken every 30 min during the first 3 h of incubation and then at 4, 5, 6, and 24 h after inoculation.…”

“…The early-stage biofilm formation includes initial adhesion and microcolony formation; both are important to the subsequent development of biofilm architecture and the physiology of biofilm cells (15)(16)(17)(18)(19). The generally accepted model is that motile bacteria use cell surface appendages, such as flagella and pili, to explore a surface and make the decision of switching from planktonic growth to biofilm formation (20)(21)(22)(23).…”

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

“…Candida albicans has 30 genes in which mutations could decrease adherence of hyphae. In addition to their genetic profile, the surface topography and host immunology are important factors controlling Candida albicans adherence [ 19 , 20 ]. Immune-competent cells present on the VP surface increase their migration upon activation by biofilm components [ 21 ].…”