Impact of submerged culture conditions on growth and bioactive metabolite produced by endophyte Hypocrea spp. NSF-08 isolated from Dillenia indica Linn. in North-East India

“…Miq.were tested against four gram-positive, Seven gram-negative bacteria and against three pathogenic fungi. n-Hexane and dichloromethane fractions showed remarkable activities against all the tested bacteria however, nhexane fraction showed highest activity against Shigella dysenteriae 60,67,84 . Adding on, the antibacterial and antifungal activity of the crude extracts as well as for the isolated pure compounds has been checked using fifteen bacterial strains, which included seven gram-positive and eight gram-negative organisms, and nine fungi.…”

Section: According Tomentioning

confidence: 94%

A Review Update on Dillenia Indica F.ELONGATA (Miq.)miq.

Boparai

Niazi

Bajwa

et al. 2016

J. Drug Delivery Ther.

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Dillenia indica f. elongata (Miq.) Miq. (Dilleniaceae) commonly known as Elephant apple. The vernacular names include Chalta, Chulta, Karambel, Bhavya and Ramphal.It is found in Bangladesh, Nepal, China, Indonesia and Assam, West Bengal, Orissa, Bihar, Myanmar regions of India. Dillenia indica f. elongata (Miq.) Miq.has been prevalently used in Indian traditional and ayurvedic medicine for curing plethora of ailments such as digestive, respiratory and central nervous systems. The information was put together, with the help of literature surveyed. Traditionally different parts of Dillenia indica f. elongata (Miq.) Miq. have been used for the relief of indigestion, asthma, influenza, dysentery, jaundice, promeho, weakness and rheumatic pain. Moreover, the extractives showed significant cytotoxic, CNS depressant and free radical scavenging activity. Wood obtained from the bark of the plant has been used for preparing rafters, tool-handles, cupboard and house pots. It is interesting to know that bark of Dillenia indica f. elongata (Miq.) Miq.is used for production of charcoal. Microspheres of metformin hydrochloride and novel mucoadhesive buccal tablets of oxytocin were also prepared from Dillenia indica f. elongata (Miq.) Miq. In vitro propagation is practised whereas micropropagation is considered at the time, when large scale plant multiplication is required. Phytochemical studies revealed substantial presence of polyphenols, tannins, alkaloids and flavanoids which are responsible for the various pharmacological activities. Major chemical constituents present in the plant are betulin (pentacyclic triterpenoid) and betulinic acid that show wide spectrum of pharmacological activities which include anti-HIV, anti-inflammatory, anti-cancer, anti-malarial etc. Furthermore, Dillenia indica f. elongata (Miq.) Miq. is found to possess analgesic, anti-diabetic, anti-microbial,anti-bacterial,anti-diabetic,antioxidant,anti-proliferation,anti-diarrhoeal, antiimplantation, cytotoxic, wound healing and hair waving activity.

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“…Since Eucalyptus is highly lignified, endophytic fungi are co-evolved with its host 22 . By definition, endophytes are those microbes colonizing healthy plant tissues 6 . To colonize healthy plant tissues and stay symbiotically with plants, it is expected that endophytic fungi possess and produce enzymes which can break up barriers of plant tissues and stay harmoniously.…”

Section: Antimicrobial Assay By Well and Disc Diffusion Methodmentioning

confidence: 99%

“…Endophytic fungi have recently gained importance due to their ability to produce bioactive metabolites, immuno-suppressants, anti-cancer compounds and biocontrol agents 6 . There are reports on endophytic fungi from Eucalyptus plants where they focused on density of colonization, differences in population of endophytes and sometimes production of extracellular oxidative enzymes 7 .…”

mentioning

confidence: 99%

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2012

IJPSR

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A total of thirty endophytic fungi were isolated from leaves and twigs of Eucalyptus globulus and Eucalyptus citriodora. Among thirty endophytic fungal isolates, four (P3MT1, P3MT2, OP4MT2 and P7ML2) are consistently producing compounds which are inhibiting Pseudomonas aeroginosa, Mycobacterium smegmatis and Candida albicans even after 10 generations tested under dual culture, well diffusion and disc diffusion methods. P3MT1 and OP4MT2 are inhibiting even a filamentous fungi Penicillium chrysogenum. The fungal isolate OP4MT2 showed highest zone of inhibition (20 mm) against Penicillium chrysogenum among two test fungi. The crude ethyl acetate extract of P3MT1 isolate showed highest zone of inhibition against Candida albicans (19 mm) by both well and disc diffusion method when compared to other fungal isolates. Another four fungal isolates (P3ML1, P6MT1, P5MT1 and P2MT1) from the same set of thirty isolates showed positive for the secretion of amylase, protease and laccase enzymes in agar plate method. Two endophytic fungal isolates (P6MT1 & P2MT1) among thirty are able to oxidize guaiacol indicating the presence of Lignin degrading enzymes. Four fungal isolates indicated presence of laccase enzymes by qualitative test were able to decolorize both methylene blue and aniline blue (synthetic dyes) in solid and liquid media. The quantitative estimation of percent decolorization of synthetic dyes by spectrophotometric method confirmed more than 90 % reduction in color is made possible by the endophytic fungi. All these fungal strains with good bioactivity are of worth studying in detail for the purification and characterization of the active compounds and enzymes. INTRODUCTION: Eucalyptus genus belonging to the family of Myrtaceae grows rapidly to a gigantic size and is one among the tallest trees of the world 1. There are about 500 species of Eucalyptus plants which produce essential oils 2. Eucalyptus oil has antibacterial, antiviral and anti fungal components and used for the treatment of cold, influenza, rhinitis, sinusitis and other respiratory infections 3. Almost all the plants which were studied are known to support a vast group of microorganisms called endophytes living inside the live parts of plants 4. Endophytic fungi are one among these endophytes living inside the plants without causing any apparent symptoms 5 .

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Impact of submerged culture conditions on growth and bioactive metabolite produced by endophyte Hypocrea spp. NSF-08 isolated from Dillenia indica Linn. in North-East India

Cited by 15 publications

References 36 publications

Effect of fructose on promoting fengycin biosynthesis inBacillus amyloliquefaciensfmb-60

Effect of fructose on promoting fengycin biosynthesis inBacillus amyloliquefaciensfmb-60

A Review Update on Dillenia Indica F.ELONGATA (Miq.)miq.

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