Impact of Sterilization Exposure Time on Leaf Explants of Anthurium cv. Xavia for Establishing an Aseptic Culture

Toppo, Rashmita; Beura, Sashikala

doi:10.20546/ijcmas.2018.709.354

Cited by 1 publication

(2 citation statements)

References 4 publications

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“…The carbon source (sucrose 30 g/l) and gelling agent (Hi-media agar powder 8 g/l). A pH of 5.8 was adjusted with 0.1 N HCl or 0.1 NaOH [8]. The medium was immediately poured into glass tubes of 18 x 150 mm up to 20 ml in each tube.…”

Section: Preparation Of Chu Mediamentioning

confidence: 99%

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Hormonal Effect on Callus Induction and Shoot Multiplication on Leaf Explants in Gerbera (Gerbera jamesonii) Cultivar Grand

Kumar

Rajbher²,

Shukla³

et al. 2022

IJPSS

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Aims: Gerbera is very popular as a cut flower in India. Due to it is multi color its very attractive and it has longer vase life. It is a dwarf, stem-less, herbaceous, perennial plant that is commercially propagated by the cell culture. Methodology: The research was conducting an investigation into In vitro method used nutrient culture media under control condition. In-vitro method were observed that the effect of 2,4-D +Kinetin and 2,4-D + BAP with different treatment concentrations on callus induction and shoot multiplication under In vitro condition was carried out using leaf explants. Results: The result acquired has shown that minimum time (7.18 days) was noted under the treatment combination 2,4-D @ 2.00 mgl-1+ Kinetin @1.50 mgl-1 whereas maximum time (35.57 days) was found of callus with the treatment combination 2,4-D @2.00 mgl-1+ BAP @2.00 mgl-1under control. After the callus induction, the callus was transferred for the establishment of the shoot on different shoot multiplication media showed that time of shoot initiation 7.18 days. shoot per cent was 76.65 in these days also the number of shoots per explant 11.23 along with this, the development of shoot was completed in 11.74 days when Length of the shoot was viewed on 25 days to shoot the length was 2.17 cm, 35 days after 3.60 and 45 days after 4.56 cm. and after that count of shoot per culture was 12.62 were observed on treatment of CHU Media + BAP @ 2.50 mgl-1+Kinetin @ 1.50 mgl-1.

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Section: Preparation Of Chu Mediamentioning

confidence: 99%

“…The explants were treated with 4% sodium hypochlorite for 6-7 minutes followed by treatment of 0.1 per cent Mercuric Chloride (HgCl 2 ) for 5 minutes. After sterilization, explants were placed on a filter paper for absorption of extra water [8].…”

Section: Sterilization Of Explantsmentioning

confidence: 99%

Hormonal Effect on Callus Induction and Shoot Multiplication on Leaf Explants in Gerbera (Gerbera jamesonii) Cultivar Grand

Kumar

Rajbher²,

Shukla³

et al. 2022

IJPSS

View full text Add to dashboard Cite

show abstract

Impact of Sterilization Exposure Time on Leaf Explants of Anthurium cv. Xavia for Establishing an Aseptic Culture

Abstract: and pockets of North Eastern parts of India under semishade condition. In Odisha also cut anthurium flowers are widely used in bouquets and flower arrangements and mainly they are procured from the neighbouring states as its

Cited by 1 publication

References 4 publications

Hormonal Effect on Callus Induction and Shoot Multiplication on Leaf Explants in Gerbera (Gerbera jamesonii) Cultivar Grand

Hormonal Effect on Callus Induction and Shoot Multiplication on Leaf Explants in Gerbera (Gerbera jamesonii) Cultivar Grand

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