Impact of solubility on laser tissue-welding with albumin solid solders

Lauto, Antonio; Stewart, Robert B.; Ohebshalom, Michael; Nikkoi, N.D.; Felsen, Diane; Poppas, Dix P.

doi:10.1002/1096-9101(2001)28:1<44::aid-lsm1015>3.0.co;2-w

Cited by 13 publications

(11 citation statements)

References 25 publications

(7 reference statements)

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“…Hence, the fluence ($52 J/cm 2 ) characterizes the LTR of chitosan adhesives better than the radiation dose (J/mg). In contrast, the latter is more appropriate for albumin solders, which undergo a phase transition during denaturation and they require a fixed amount of energy per mole to change the protein conformation (1.2 AE 0.5 J/g) [21].…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

et al. 2006

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Background and Objectives: Sutures are currently the gold standard for wound closure but they are still unable to seal tissue and may induce scarring or inflammation. Biocompatible glues, based on polysaccharides such as chitosan, are a possible alternative to conventional wound closure. In this study, the adhesion of laser-activated chitosan films is investigated in vitro and in vivo. In particular we examine the effect of varying the laser power, as well as adding a natural cross-linker (genipin) to the adhesive composition. Study Design/Materials and Methods: Flexible and insoluble strips of chitosan films (surface area $34 mm 2 , thickness $20 mm) were bonded to sheep intestine using several laser powers (0, 80, 120, and 160 mW) at 808-nm wavelength. The strength of repaired tissue was tested by a calibrated tensiometer to select the best power. A natural cross-linker (genipin) was also added to the film and the tissue repair strength compared with the strength of plain films. The adhesive was also bonded in vivo to the sciatic nerve of rats and the thermal damage induced by the laser assessed 4 days post-operatively. Results: Chitosan adhesives successfully repaired intestine tissue, attaining a maximum repair strength of 14.7 AE 4.3 kPa (n ¼ 30) at the laser power of 120 mW. The chitosan-genipin films achieved lower repair strength (9.1 AE 2.9 kPa). The laser caused partial demyelination of axons at the site of operation, but the myelinated axons retained a normal morphology proximally and distally. Conclusions: The chitosan adhesive effectively bonded to tissue causing only localized thermal damage in vivo, when the appropriate laser parameters were selected.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

et al. 2006

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“…It has been previously shown that a solid protein strip dissolves a few micron of its thickness when in contact with tissue moisture and therefore, is equivalent to a concentrated fluid solder at the tissue interface [5]. In this report, the solid solder was preferred over the fluid one since it allowed control of the solder thickness and surface area with much higher precision.…”

Section: Discussionmentioning

confidence: 95%

“…The major drawback of this technique was the weak tensile strength of the repairs due to the decreased solubility of the partially denatured solder. Indeed, it has recently been reported by Lauto et al that repair strength is reduced when solder solubility decreases [5].…”

Section: Introductionmentioning

confidence: 98%

Albumin–genipin solder for laser tissue repair

Lauto¹,

Foster²,

Ferris³

et al. 2004

Lasers Surg Med

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“…Reports suggest a variety of contributing factors from non-covalent, covalent, and disulfide bonds through denaturation of the collagen substructure and the formation of mechanical interlocks as the protein solder and adjacent tissues denature [18,20,21]. Our experience with the BioWeld 1 device has shown that by far the most important mechanism for vascular fixation is geometric in origin rather than chemical.…”

Section: Discussionmentioning

confidence: 99%

Laser‐assisted end‐to‐end bioweld^® anastomosis in an ovine model

Wright¹,

Vicaretti

Schwaiger³

et al. 2007

Lasers Surg Med

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The BioWeld tube, an albumin-based exovascular stent, has been used for microsurgical anastomoses and compared to conventional sutures. The study presented investigated the potential of the BioWeld tube for vascular anastomosis in larger vessels. Laser-assisted BioWeld anastomoses were compared to conventional-sutured anatomoses of the carotid artery of Merino-x ewes. The BioWeld procedure resulted in 100% survival and 100% patency at 1 and 6 week post-operative periods, with no noticeable foreign body response. Sutured animals showed 100% survival and patency. The ischemic time for BioWeld anastomosis averaged 15 minutes compared with 10 minutes for sutures. This study indicates that the BioWeld tube is an easy to use anastomotic technique with equivalent success rates and comparable anastomotic times.

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Impact of solubility on laser tissue-welding with albumin solid solders

Abstract: The soluble solid solder behaved like dense liquid solder at the tissue interface. Hence, the interface strength of these two forms of solder should be similar. This correlation made it possible to identify an intrinsic limit for the weld strength of albumin solders.

Cited by 13 publications

References 25 publications

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

Albumin–genipin solder for laser tissue repair

Laser‐assisted end‐to‐end bioweld^® anastomosis in an ovine model

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Impact of solubility on laser tissue-welding with albumin solid solders

Abstract: The soluble solid solder behaved like dense liquid solder at the tissue interface. Hence, the interface strength of these two forms of solder should be similar. This correlation made it possible to identify an intrinsic limit for the weld strength of albumin solders.

Cited by 13 publications

References 25 publications

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

In vitro and in vivo tissue repair with laser‐activated chitosan adhesive

Albumin–genipin solder for laser tissue repair

Laser‐assisted end‐to‐end bioweld® anastomosis in an ovine model

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Product

Resources

About

Laser‐assisted end‐to‐end bioweld^® anastomosis in an ovine model