This paper investigated the demographic determinants of job satisfaction among non-academic senior staff (referred to as senior staff) of the University of Cape Coast, Ghana. By employing the descriptive design with a quantitative approach, we used a sample of 302 senior staff who were selected through multi-stage sampling procedures. An adapted version of the Minnesota Satisfaction Questionnaire was used to gather data. One-way between groups Analysis of Variance (ANOVA), and independent samples t-test were used to analyse the data. Findings revealed a statistically significant difference in the job satisfaction of senior staff concerning their years of working experience [F(2, 300) = 9.940, p< 0.001)], with senior staff who had 6-10 years of work experience being more satisfied with their jobs (Mean = 175.20). Again, the paper established a statistically significant difference in the job satisfaction of senior staff regarding their academic qualification [F(2, 300) = 8.357, p = 0.043], with senior staff who had second degree being more satisfied (Mean = 183.61). However, our results revealed no statistically significant difference in job satisfaction of senior staff concerning their gender [t (300) = -0.384, p = .701], and rank [F(2, 300) = -4.462, p = 0.061]. We concluded from the results that while some demographic variables (academic qualification and years of work experience) determine senior staff’s job satisfaction, others (gender and rank) do not. We therefore, recommend that managers and administrators of tertiary educational institutions should develop strategies to enhance working conditions that will ensure opportunities for further studies, higher responsibilities and promotion.