Impact of six fruits—banana, guava, mangosteen, pineapple, ripe mango and ripe papaya—on murine hepatic cytochrome P450 activities

Abstract

The effects of six Thai fruits, namely banana, guava, mangosteen, pineapple, ripe mango and ripe papaya, on cytochrome P450 (P450) activities were investigated. The median inhibitory concentrations (IC50) of each of the fruit juices on CYP1A1, CYP1A2, CYP2E1 and CYP3A11 activities were determined. Pineapple juice showed the strongest inhibitory effect against all the evaluated P450 isozyme activities in mouse hepatic microsomes, followed by mangosteen, guava, ripe mango, ripe papaya and banana. The study was further performed in male ICR mice given pineapple juice intragastrically at doses of 10, 20 and 40 mg kg−1 per day for 7 or 28 days. In a concentration‐dependent fashion, the pineapple juice raised ethoxyresorufin O‐deethylase, aniline hydroxylase and erythromycin N‐demethylase activities, which are marker enzymatic reactions responsible for CYP1A1, CYP2E1 and CYP3A11, respectively. The effect of pineapple juice on the expression of CYP1A1, CYP2E1 and CYP3A11 mRNAs corresponded to their enzymatic activities. However, the pineapple juice significantly decreased methoxyresorufin O‐demethylase activity. These observations supported that the six Thai fruits were a feasible cause of food–drug interaction or adverse drug effects owing to their potential to modify several essential P450 activities. Individuals consuming large quantities of pineapple for long periods of time should be cautioned of these potential adverse effects. Copyright © 2012 John Wiley & Sons, Ltd.

Mutagenicity and Antimutagenicity of Hydrophilic and Lipophilic Extracts of Thai Northern Purple Rice

“…For example, flavonoids containing a methoxyl group can induce CYP1A1 enzyme, which is an enzyme involved in benzo(a)pyrene metabolism (Androutsopoulos 2011). Moreover, some flavonols and flavones inhibited CYP1A2 activity, but enhanced CYP1A1 acitivity (Moon et al, 2006;Chatuphonprasert and Jarukamjorn, 2012). In addition, previous studies have emphasized that flavonoids act as AhR antagonists or agonists depending on their structure and concentration (Hodex et al, 2002).…”

Effect of tetrahydrocurcumin on the profiles of drug-metabolizing enzymes induced by a high fat and high fructose diet in mice

“…Hepatic microsomal protein was prepared as described [17]. Immunoblotting analysis was employed to determine the level of the protein expression of the CYPs [18] with some modification.…”

Thai red rice extract provides liver protection in paracetamol-treated mice by restoring the glutathione system

“…The total phenolic content of the colored rice extract was determined using the Folin-Ciocalteu method (Chatuphonprasert & Jarukamjorn, 2012). The rice extract was thoroughly mixed with Folin-Ciocalteu reagent and subjected to spectrophotometry at a wavelength of 750 nm.…”

“…The aluminum chloride colorimetric method was applied with some modification for determination of total flavonoid content (Chatuphonprasert & Jarukamjorn, 2012). The reaction mixture contained 10% aluminum chloride, 1 M sodium acetate, and the rice extract in a final volume of 200 mL.…”