Impact of silver nanoparticles size on SERS for detection and identification of filamentous fungi

Ramirez-Perez, Javier C.; Alves, T. C.; Olivera, Cristiano; Rizzutto, M. A.

doi:10.1016/j.saa.2022.120980

Cited by 18 publications

(11 citation statements)

References 30 publications

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“…The UV–Vis absorbance peak was detected at 415 nm as shown in Figure 2 b, and the full width at half maximum (FWHM) of approximately 76 nm shows a relatively good uniformity of AgNP size. Both size and uniformity of AgNPs indicated good characteristics for the formation of “hotspots” [ 15 , 16 ]. These characteristics were maintained relatively stable for 6 months, which represented acquired stability for TLC-SERS measurement.…”

Section: Resultsmentioning

confidence: 99%

Single versus Double Coffee-Ring Effect Patterns in Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopic Analysis of Anti-Diabetic Drugs Adulterated in Herbal Products

Minh,

Tram,

Phong

et al. 2023

Molecules

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In thin-layer chromatography coupled with surface-enhanced Raman spectroscopy (TLC-SERS), the coffee ring effect (CRE) describes the formation of a ring-shape spot (blank in the middle and darker on the edge) caused by the aggregation of silver nanoparticles (Ag NPs), alone (single CRE) or with the analytes (double CRE). In this work, the SCRE and DCRE were investigated in two anti-diabetic drugs, hydrophobic glibenclamide (GLB) and more hydrophilic metformin (MET). The SCRE occurred in GLB analysis, as opposed to the DCRE that occurred in MET. It was proven that for optimization of the TLC-SERS analytical procedure, it is necessary to distinguish the CRE patterns of analytes. Additionally, MET and GLB were analyzed with the developed TLC-SERS method and confirmed by another validated method using high-performance liquid chromatography. Four herbal products collected on the market were found to be adulterated with GLB or/and MET; among those, one product was adulterated with both MET and GLB, and two products were adulterated with GLB at a higher concentration than the usual GLB prescription dose. The TLC-SERS method provided a useful tool for the simultaneous detection of adulterated anti-diabetic herbal products, and the comparison of the SCRE and DCRE provided more evidence to predict CRE patterns in TLC-SERS.

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Section: Resultsmentioning

confidence: 99%

Single versus Double Coffee-Ring Effect Patterns in Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopic Analysis of Anti-Diabetic Drugs Adulterated in Herbal Products

Minh,

Tram,

Phong

et al. 2023

Molecules

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“…Keissl., Aspergillus flavus Link, Fusarium verticillioides (Sacc.) Nirenberg, Aspergillus parasiticus Speare) previously analyzed by SERS [31,34]. In addition, these Raman spectra showed a high similarity with the SERS of some of the pigments existing in the fungal cell wall, such as flavonoid pigments [54].…”

Section: Normal Raman and Sers Spectroscopymentioning

confidence: 87%

“…These "hot spots" result from plasmonic coupling between NPs and increase rapidly as the interparticle distance becomes shorter [21]. As a result of their efficacy the SERS-based metallic NPs have been used to characterize complex biological samples such as nucleic acids [22][23][24] and proteins [25][26][27] as well as for the study of different microorganisms, including fungi [28][29][30][31][32], yeasts [33], and plant pathogens [34,35]. Moreover, SERS spectroscopy coupled with the PCA has been employed by several authors to discriminate different fungal species on the basis of their spectra [30,31,34].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic analysis of mushrooms by surface-enhanced Raman scattering (SERS)

Puliga

Zuffi

Zambonelli

et al. 2022

Chem. Biol. Technol. Agric.

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Background Mushrooms have always been considered an important source of food and biologically active compounds with several medicinal properties. In recent years, different methods were used to study the quality and chemical composition of mushrooms. Among these, Fourier transform infrared (FT-IR) and FT-Raman spectroscopy techniques have been successfully applied to identify different mushroom species. However, the structural biomolecule components existing in the mycelium or in the fruiting bodies may produce strong fluorescence emission that overlaps the Raman radiation, thus avoiding their analyses by Raman. SERS spectroscopy is a powerful technique which uses metal nanoparticles (NPs) to enhance the Raman signal of molecules adsorbed on the NPs surface. In addition, SERS is able to quench the macromolecule florescence. In this work, we have employed silver nanoparticles in order to get mushroom fingerprints based on SERS as quick procedure to analyze and identify different chemical compounds from the fruiting bodies of six edible and/or medicinal mushrooms: Lentinula edodes, Ganoderma lucidum, Pleurotus cornucopiae, Pleurotus ostreatus, Tuber aestivum and Tuber magnatum. Results SERS analyses performed directly on fruiting body fragments produced characteristic spectra for each species. One group of mushrooms (L. edodes, G. lucidum, T. aestivum and T. magnatum) was dominated by the bands of nucleic acids; and the other one (P. cornucopiae and P. ostreatus), by the bands of pigments such as melanins; carotenoids; azafilones; polyketides; and flavonoids located in the cell wall. Additionally, bands corresponding to cell wall polysaccharides, particularly chitosan and 1,3-β D-glucan, were identified in the extracts of P. cornucopiae, P. ostreatus and L. edodes. No signal of cell wall polysaccharides was found in G. lucidum extract. Raman mapping of the analyzed samples was useful in tracking the spatial distribution of the marker bands. Moreover, the principal component analysis (PCA) carried out on the acquired SERS spectra, allows to discriminate the analyzed mushroom species. Conclusions The SERS technique has the ability to generate a strong Raman signal from mushroom fruiting bodies using Ag-NPs deposited directly on intact, untreated mushroom tissues. Using this methodology, commonly applied laboratory time-consuming methods can be avoided or bypassed as well as analysis time can be reduced. Graphical Abstract

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“…Centrifugation was performed at 6000 rpm for 10 min for the removal of the supernatant. Now, the synthesized Ag NPs were characterized through SEM and TEM, , and the results are provided in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

Wasim,

Ghaffar,

Javed

et al. 2024

ACS Omega

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In this study, surface-enhanced Raman spectroscopy (SERS) technique, along with principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA), is used as a simple, quick, and cost-effective analysis method for identifying biochemical changes occurring due to induced mutations in the Aspergillus niger fungus strain. The goal of this study is to identify the biochemical changes in the mutated fungal cells (cell mass) as compared to the control/nonmutated cells. Furthermore, multivariate data analysis tools, including PCA and PLS-DA, are used to further confirm the differentiating SERS spectral features among fungal samples. The mutations are caused in A. niger by the clustered regularly interspaced palindromic repeat CRISPR-Cas9 genomic editing method to improve their biotechnological potential for the production of cellulase enzyme. SERS was employed to detect the changes in the cells of mutated A. niger fungal strains, including one mutant producing low levels of an enzyme and another mutant producing high levels of the enzyme as a result of mutation as compared with an unmutated fungal strain as a control sample.

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Impact of silver nanoparticles size on SERS for detection and identification of filamentous fungi

Cited by 18 publications

References 30 publications

Single versus Double Coffee-Ring Effect Patterns in Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopic Analysis of Anti-Diabetic Drugs Adulterated in Herbal Products

Single versus Double Coffee-Ring Effect Patterns in Thin-Layer Chromatography Coupled with Surface-Enhanced Raman Spectroscopic Analysis of Anti-Diabetic Drugs Adulterated in Herbal Products

Spectroscopic analysis of mushrooms by surface-enhanced Raman scattering (SERS)

Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

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