Impact of silver nanoparticles on natural marine biofilm bacteria

Fabrega, Julia; Zhang, Rui; Renshaw, Joanna C.; Liu, Wen Tso; Lead, Jamie R.

doi:10.1016/j.chemosphere.2011.06.066

Cited by 111 publications

(73 citation statements)

References 30 publications

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“…As a result of aggregation, surface area and dissolution potential are reduced. Capped nanoparticles have enhanced colloidal stability and so capped and uncapped particles may behave differently from each other (Badawy et al 2010;Fabrega et al 2011). In addition, the capping agent can affect the size and surface charge of particles which may affect particle toxicity which in turn affect the diversity and activity of microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver

Beddow

Stolpe

Cole

et al. 2014

International Biodeterioration & Biodegradation

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a b s t r a c tLittle is currently known about the potential impact of silver nanoparticles (AgNPs) on estuarine microbial communities. The Colne estuary, UK, is susceptible to oil pollution through boat traffic, and there is the potential for AgNP exposure via effluent discharged from a sewage treatment works located in close proximity. This study examined the effects of uncapped AgNPs (uAgNPs), capped AgNPs (cAgNPs) and dissolved Ag 2 SO 4 , on hydrocarbon-degrading microbial communities in estuarine sediments. The uAgNPs, cAgNPs and Ag 2 SO 4 (up to 50 mg L À1 ) had no significant impact on hydrocarbon biodegradation (80e92% hydrocarbons were biodegraded by day 7 in all samples). Although total and active cell counts in oil-amended sediments were unaffected by silver exposure; total cell counts in non-oiled sediments decreased from 1.66 to 0.84 Â 10 7 g À1 dry weight sediment (dws) with 50 mg L À1 cAgNPs and from 1.66 to 0.66 Â 10 7 g À1 dws with 0.5 mg L À1 Ag 2 SO 4 by day 14. All silver-exposed sediments also underwent significant shifts in bacterial community structure, and one DGGE band corresponding to a member of Bacteroidetes was more prominent in non-oiled microcosms exposed to 50 mg L À1 Ag 2 SO 4 compared to non-silver controls. In conclusion, AgNPs do not appear to affect microbial hydrocarbon-degradation but do impact on bacterial community diversity, which may have potential implications for other important microbial-mediated processes in estuaries.

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Section: Discussionmentioning

confidence: 99%

Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver

Beddow

Stolpe

Cole

et al. 2014

International Biodeterioration & Biodegradation

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“…Therefore, data on NP toxicity using planktonic bacteria are inappropriate for predicting the toxic effects of NPs on biofilms (Costerton et al 1995). Thus, the potential adverse effects of NPs on biofilms must be addressed, and to date, few studies have investigated this problem (Costerton et al 1995;Fabrega et al 2009Fabrega et al , 2011Choi et al 2010;Dror-Ehre et al 2010;Rodrigues and Elimelech 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the cytotoxic effect of polystyrene latex nanoparticles on planktonic cells and bacterial biofilms

et al. 2016

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The cytotoxic effect of positively charged polystyrene latex nanoparticles (PSL NPs) was compared between planktonic bacterial cells and bacterial biofilms using confocal laser scanning microscopy, atomic force microscopy, and a colony counting method. Pseudomonas fluorescens, which is commonly used in biofilm studies, was employed as the model bacteria. We found that the negatively charged bacterial surface of the planktonic cells was almost completely covered with positively charged PSL NPs, leading to cell death, as indicated by the NP concentration being greater than that required to achieve single layer coverage. In addition, the relationship between surface coverage and cell viability of P. fluorescens cells correlated well with the findings in other bacterial cells (Escherichia coli and Lactococcus lactis). However, most of the bacterial cells that formed the biofilm were viable despite the positively charged PSL NPs being highly toxic to planktonic bacterial cells. This indicated that bacterial cells embedded in the biofilm were protected by self-produced extracellular polymeric substances (EPS) that provide resistance to antibacterial agents. In conclusion, mature biofilms covered with EPS exhibit resistance to NP toxicity as well as antibacterial agents.

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“…However, one disadvantage is the selection of the restriction enzymes because even when the enzymes used are optimized, many microbial species may present the same TRFs length (Marsh 1999;Dahllöf 2002). In the nanotoxicology context, T-RFLP analysis has been applied to evaluate the environmental impact of silver nanoparticles on marine bacteria biofilms (Fabrega et al 2011) and toxicological effect of ZnO and TiO 2 nanoparticles on soil bacteria communities (Ge et al 2011).…”

Section: Culture-independent Methods Of Community Analysismentioning

confidence: 99%

“…The biofilm formation was stained using Syto 9/PI and the image acquisition of the biofilms was performed by confocal microscopy. Additionally, other studies have been described the anti-biofilm activity of functionalized multi-walled carbon nanotubes (Qi et al 2011), graphene oxide (Carpio et al 2012), and silver nanoparticles (Dror-Ehre et al 2010;Fabrega et al 2011) using the live/dead assay as protocol to quantify the percentage of living cells.…”

Section: Live/dead Cell Stainingmentioning

confidence: 98%

Toxicity of Nanomaterials to Microorganisms: Mechanisms, Methods, and New Perspectives

2013

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In the last few years, several nanomaterials with unique physicochemical properties have been developing. Specially, nano-sized materials such as silver and zinc nanoparticles, carbon nanotubes, and graphene oxide have been attracting great attention due to their potential as novel antimicrobial agents. Worldwide, the constant and indiscriminate use of conventional antibiotics has been responsible for the development of several resistant microbial species. In this context, there is a real and increasing demand for new antimicrobial agents. Nanomaterials offer several benefits due to their small size (high aspect volume/ area) that provides to nanoparticles great ability to get through physical barriers such as membranes and cellular walls. Henceforth, the aim of this present chapter is to discuss the toxicological aspects of nanomaterials to microorganisms, describing the methods to evaluate their antimicrobial activity and highlighting their implications on the microbial communities of soil and water environments. We also stress the main industrial applications of antimicrobial-engineered nanomaterials.

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Impact of silver nanoparticles on natural marine biofilm bacteria

Cited by 111 publications

References 30 publications

Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver

Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver

Comparison of the cytotoxic effect of polystyrene latex nanoparticles on planktonic cells and bacterial biofilms

Toxicity of Nanomaterials to Microorganisms: Mechanisms, Methods, and New Perspectives

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