Impact of shelf-life simulation on bonding performance of universal adhesive systems

Cuevas‐Suárez, Carlos Enrique; Ramos, T.S.; Rodrigues, Stéfani Becker; Collares, Fabrício Mezzomo; Zanchi, César Henrique; Lund, Rafael Guerra; Silva, Adriana Fernandes da; Piva, Evandro

doi:10.1016/j.dental.2019.05.023

Cited by 23 publications

(36 citation statements)

References 23 publications

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“…All have phosphated acid monomers in their composition and are considered 'ultra mild' based on their pH (SBU: pH=2.7; FTU: pH=2.5 and TET: pH=2.5-3.0) (13,1), which makes them capable of demineralizing and diffusing in the dentin, forming a hybrid layer that is more stable to hydrolytic degradation due to changes in their chemical composition (11). The phosphated functional monomers in their composition chemically interact with hydroxyapatite forming hydrolytic and more stable bonds with calcium (14,15,16). It is reported that adhesive systems containing MDP phosphated monomers chemically interact with hydroxyapatite forming 10-MDP-Ca salts that have low solubility, better resistance to hydrolysis, and are more stable.…”

Section: Discussionmentioning

confidence: 99%

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Influence of Acid Etching and Universal Adhesives on the Bond Strength to Dentin

et al. 2020

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The purpose of this study was to evaluate the influence of the application mode of three universal adhesive systems on interfacial physical properties of indirect composite restorations adhesively cemented to dentin cavities. Seventy-eight bovine lower incisors were selected and a slice of dentin (thickness: 2 mm) between the buccal surface and pulp chamber was obtained for each tooth. Conical cavities were made on this surface. The internal walls of the cavities were then coated with a hydrophilic gel, filled with composite resin and photopolymerized. The dentin/cone sets were divided into 6 groups (n=10) according to type of universal adhesive (TETRI: Tetric N Bond, FUT: Futura Bond U, SBU: Single Bond Universal) and acid etching on dentin (A: with acid etching; WA: without acid etching). The acid etching and the adhesive systems were applied to the surface of the dentin. All composite resin cones were sandblasted (Al2O3, 20 s) and silanized. After surface treatment, the cones were cemented (RelyX Ultimate) into the dentin cavity and photopolymerized. After thermocycling (10,000 cycles), samples were submitted to marginal adaptation analysis (using caries detector dye), push-out test (0.5 mm/min), and failure mode analysis. Additional samples were prepared for nanoleakage analysis (SEM). The data (MPa) were analyzed by two-way ANOVA and Tukey’s post-test (5%). The groups in which the dentin was acid etched showed significantly lower bond strength values in the push-out test (p<0.01). Dentin acid etching significantly reduced the bond strength between universal adhesive systems and dentin in indirect restorative procedures.

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Section: Discussionmentioning

confidence: 99%

“…Varied results are found in the literature (19,21,15). One study (22) reported that dentin conditioning had no negative impact on adhesion.…”

Section: Discussionmentioning

confidence: 99%

Influence of Acid Etching and Universal Adhesives on the Bond Strength to Dentin

et al. 2020

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“…The concept provided more choices regarding the adhesion strategy; self-etch, total-etch or selective enamel-etch mode (total-etch on enamel and self-etch on dentin) [11,12]. However, a hurdle is presented when formulating universal adhesives, since water, which might be needed inside adhesive systems, may lead to the degradation of the chemical formulation of these systems, contributing to phase separation and reducing shelf life [13].…”

Section: Introductionmentioning

confidence: 99%

Reinforced Universal Adhesive by Ribose Crosslinker: A Novel Strategy in Adhesive Dentistry

et al. 2021

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Enzymatic biodegradation of demineralized collagen fibrils could lead to the reduction of resin–dentin bond strength. Therefore, methods that provide protection to collagen fibrils appear to be a pragmatic solution to improve bond strength. Thus, the study’s aim was to investigate the effect of ribose (RB) on demineralized resin–dentin specimens in a modified universal adhesive. Dentin specimens were obtained, standardized and then bonded in vitro with a commercial multi-mode adhesive modified with 0, 0.5%, 1%, and 2% RB, restored with resin composite, and tested for micro-tensile bond strength (µTBS) after storage for 24 h in artificial saliva. Scanning electron microscopy (SEM) was performed to analyze resin–dentin interface. Contact angles were analyzed using a contact angle analyzer. Depth of penetration of adhesives and nanoleakage were assessed using micro-Raman spectroscopy and silver tracing. Molecular docking studies were carried out using Schrodinger small-molecule drug discovery suite 2019-4. Matrix metalloproteinases-2 (MMP-2) and cathepsin-K activities in RB-treated specimens were quantified using enzyme-linked immunosorbent assay (ELISA). The significance level was set at α = 0.05 for all statistical analyses. Incorporation of RB at 1% or 2% is of significant potential (p < 0.05) as it can be associated with improved wettability on dentin surfaces (0.5% had the lowest contact angle) as well as appreciable hybrid layer quality, and higher resin penetration. Improvement of the adhesive bond strength was shown when adding RB at 1% concentration to universal adhesive (p < 0.05). Modified adhesive increased the resistance of collagen degradation by inhibiting MMP-2 and cathepsin-K. A higher RB concentration was associated with improved results (p < 0.01). D-ribose showed favorable negative binding to collagen. In conclusion, universal adhesive using 1% or 2% RB helped in maintaining dentin collagen scaffold and proved to be successful in improving wettability, protease inhibition, and stability of demineralized dentin substrates. A more favorable substrate is created which, in turn, leads to a more stable dentin-adhesive bond. This could lead to more advantageous outcomes in a clinical scenario where a stable bond may result in longevity of the dental restoration.

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“…Recently, problems related to the conservation of the bonding agent have been emphasized, being liable to hydrolysis or difficulty in the polymerization of the monomers, degradation of the additive components (initiators/stabilizers), or evaporation of the ingredients [6,7]. While on one side the manufacturers specify to strictly follow the recommendations related to the storage instructions of the materials (temperatures, heat sources, bottle security), on the other side, failure to observe the expiry dates of the products can lead to premature deterioration of the adhesive performances [3,8]. Usually, the manufacturers of the adhesive systems provide a two-year expiry time for the products, after which they foresee a risk of physical-chemical compromise, which could prematurely invalidate their adhesive capacity [8].…”

Section: Introductionmentioning

confidence: 99%

“…While on one side the manufacturers specify to strictly follow the recommendations related to the storage instructions of the materials (temperatures, heat sources, bottle security), on the other side, failure to observe the expiry dates of the products can lead to premature deterioration of the adhesive performances [3,8]. Usually, the manufacturers of the adhesive systems provide a two-year expiry time for the products, after which they foresee a risk of physical-chemical compromise, which could prematurely invalidate their adhesive capacity [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of shelf-life of a universal adhesive to dentin

Mazzitelli

Maravić

Sebold

et al. 2020

International Journal of Adhesion and Adhesives

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The microtensile bond strength (μTBS), nanoleakage expression (NL), and endogenous enzymatic activity of a simplified universal adhesive system used in combination with a dual-cure resin cement for luting indirect restorations were evaluated considering its shelf-life (as-received vs expired). The universal adhesive was used asreceived (AS-R) by manufacturer or after three months after being expired (EXP). Resin composite overlays (n ¼ 10) were luted to flat deep coronal dentin surfaces with a universal bonding system (iBond universal, used in the self-etch mode) in combination with a dual-cure resin cement (RelyX Ultimate; 20s of light-cure followed by 15 min of self-cure at 37 � C). After 24 h, the specimens were cut into microtensile sticks (1 mm 2 ) and stressed to failure under tension, in accordance with ISO/TS 11.405. The fracture pattern was evaluated with optical microscopy. The significance level of p ¼ 0.05 was used for the statistical analysis. Additional specimens (n ¼ 4) were processed for quantitative interfacial nanoleakage expression using ammoniacal silver nitrate. To investigate the endogenous enzymatic activity within the hybrid layer in situ zymography was performed. The EXP group obtained significantly lower μTBS values than those recorded in the AS-R group (p < 0.05). NL resulted in higher deposition of AgNO 3 granules when the adhesive was used in the EXP group compared to the AS-R bonding system. In situ zymography assay revealed increased level of fluorescence when the universal bonding system was used at the end of shelf-life compared to the AS-R group. The use of the universal adhesive system beyond the expiry limit resulted in decreased bonding performances. Furthermore, the higher endogenous enzymatic activity recorded after the end of the shelf life of the universal adhesive would portend for a shortened durability of the restorations.

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Impact of shelf-life simulation on bonding performance of universal adhesive systems

Cited by 23 publications

References 23 publications

Influence of Acid Etching and Universal Adhesives on the Bond Strength to Dentin

Influence of Acid Etching and Universal Adhesives on the Bond Strength to Dentin

Reinforced Universal Adhesive by Ribose Crosslinker: A Novel Strategy in Adhesive Dentistry

Effect of shelf-life of a universal adhesive to dentin

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