Impact of selective fibronectin nanoconfinement on human dental pulp stem cells

Karakeçili, Ayşe; Messina, Grazia M. L.; Yurtsever, Merve Çapkın; Gümüşderelı́oğlu, Menemşe; Marletta, Giovanni

doi:10.1016/j.colsurfb.2014.08.008

Cited by 18 publications

(24 citation statements)

References 40 publications

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“…(E–H) and (B)]. This finding is consistent with a number of studies showing that no matter what cell types were used (human osteoblasts, SaoS‐2 cells, rat mesenchymal stem cells (rMSCs), and human dental pulp stem cells) cell spreading has been hindered by topographical features on concave surfaces such as nanoscaled (30–60 nm), submicron (120–500 nm) and micron (2 μm) pores/pits. The reduced cell spreading on porous surfaces can be attributed to the influence of pore depth.…”

Section: Discussionsupporting

confidence: 86%

A comparative study of the effect of submicron porous and smooth ultrafine‐grained Ti‐20Mo surfaces on osteoblast responses

Gui

Abraham

et al. 2018

J Biomedical Materials Res

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The surface of an orthopaedic implant plays a crucial role in determining the adsorption of proteins and cell functions. A detailed comparative study has been made of the in vitro osteoblast responses to coarse-grained (grain size: 500 μm), ultrafine-grained (grain size: 100 nm), coarse-porous (pore size: 350 nm), and fine-porous (pore size: 155 nm) surfaces of Ti-20Mo alloy. The purpose was to provide essential experimental data for future design of orthopaedic titanium implants for rapid osseointegration. Systematic original experimental data was produced for each type of surfaces in terms of surface wettability, cell morphology, adhesion, growth, and differentiation. Microscopic evidence was collected to reveal the detailed interplay between each characteristic surface with proteins or cells. Various new observations were discussed and compared with literature data. It was concluded that the coarse-porous surfaces offered the optimum topographical environment for osteoblasts and that the combination of ultrafine grains and considerable grain boundary areas is not an effective way to enhance cell growth and osteogenic capacity. Moreover, pore features (size and depth) have a greater effect than smooth surfaces on cell growth and osteogenic capacity. It proves that cells can discern the difference in pore size in the range of 100-350 nm. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2020-2033, 2018.

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Section: Discussionsupporting

confidence: 86%

A comparative study of the effect of submicron porous and smooth ultrafine‐grained Ti‐20Mo surfaces on osteoblast responses

Gui

Abraham

et al. 2018

J Biomedical Materials Res

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“…Polymer surfaces with topography introduced via electron-beam lithography or colloidal nanopatterning have proven to be particularly effective in protein confinement. [114][115][116][117] For example, nanopatterned polyhydroxymethylsiloxane (PHMS) surfaces with different sized cavities (70 and 270 nm) created by nanowell topography have been found to direct site-selective adsorption of fibrinogen molecules, namely, within and outside the cavities. [115] This selective protein nanoconfinement has been shown to correlate with human dental pulp stem cell morphology and cytoskeletal organization, and was interpreted in terms of nanotopographyinduced conformational changes.…”

Section: Protein Adsorption On Nanostructured Amorphous Polymer Surfacesmentioning

confidence: 99%

“…[114][115][116][117] For example, nanopatterned polyhydroxymethylsiloxane (PHMS) surfaces with different sized cavities (70 and 270 nm) created by nanowell topography have been found to direct site-selective adsorption of fibrinogen molecules, namely, within and outside the cavities. [115] This selective protein nanoconfinement has been shown to correlate with human dental pulp stem cell morphology and cytoskeletal organization, and was interpreted in terms of nanotopographyinduced conformational changes. Similarly, Ngandu Mpoyi et al have shown that a nanostructured PC surface comprising of 150 nm diameter pits is able to control cell differentiation, due to the surface ability to steer the nanoconfinement of fibronectin molecules.…”

Section: Protein Adsorption On Nanostructured Amorphous Polymer Surfacesmentioning

confidence: 99%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

105

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The initial host response to healthcare materials' surfaces after implantation is the adsorption of proteins from blood and interstitial fluids. This adsorbed protein layer modulates the biological/cellular responses to healthcare materials. This stresses the significance of the surface protein assembly for the biocompatibility and functionality of biomaterials and necessitates a profound fundamental understanding of the capability to control protein–surface interactions. This review, therefore, addresses this by systematically analyzing and discussing strategies to control protein adsorption on polymeric healthcare materials through the introduction of specific surface nanostructures. Relevant proteins, healthcare materials' surface properties, clinical applications of polymer healthcare materials, fabrication methods for nanostructured polymer surfaces, amorphous, semicrystalline and block copolymers are considered with a special emphasis on the topographical control of protein adsorption. The review shows that nanostructured polymer surfaces are powerful tools to control the amount, orientation, and order of adsorbed protein layers. It also shows that the understanding of the biological responses to such ordered protein adsorption is still in its infancy, yet it has immense potential for future healthcare materials. The review, which is—as far as it is known—the first one discussing protein adsorption on nanostructured polymer surfaces, concludes with highlighting important current research questions.

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“…Thus, protein adsorption is the very first biological event of cell‐material interactions. Generally, protein adsorption is affected by a range of surface properties, especially hydrophilicity and topography . For example, Ko et al reported that a hydrophilic surface could strengthen the binding of adhesion proteins to the surface.…”

Section: Discussionmentioning

confidence: 99%

“…The topographical features of material surface are known to modulate the adsorption and conformation of extracellular matrix (ECM) proteins, and adsorbed ECM proteins act as extracellular ligands to specifically bind to cell receptors and mediate cell adhesion. [30][31][32][33] Thus, protein adsorption is the very first biological event of cellmaterial interactions. Generally, protein adsorption is affected by a range of surface properties, especially hydrophilicity and topography.…”

Section: Discussionmentioning

confidence: 99%

Topographical cues of direct metal laser sintering titanium surfaces facilitate osteogenic differentiation of bone marrow mesenchymal stem cells through epigenetic regulation

Zheng

Guan

et al. 2018

Cell Proliferation

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Topographical cues of DMLS surfaces have greater potential for the induction of osteogenic differentiation of BMSCs than SLA and Ti surfaces both in vitro and in vivo. A potential epigenetic mechanism is that the appropriate topography allows rapid H3K27 demethylation and an increased H3K4me3 level at the promoter region of osteogenesis-associated genes during the osteogenic differentiation of BMSCs.

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Impact of selective fibronectin nanoconfinement on human dental pulp stem cells

Cited by 18 publications

References 40 publications

A comparative study of the effect of submicron porous and smooth ultrafine‐grained Ti‐20Mo surfaces on osteoblast responses

A comparative study of the effect of submicron porous and smooth ultrafine‐grained Ti‐20Mo surfaces on osteoblast responses

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Topographical cues of direct metal laser sintering titanium surfaces facilitate osteogenic differentiation of bone marrow mesenchymal stem cells through epigenetic regulation

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