Impact of Selected Plant Growth Regulators on Rooting Response of Stem Cuttings of Psidium guajava L.

Abstract

Guava can be propagated vegetatively through layering, cutting and grafting, in addition to seeds. Among the propagation techniques, cuttings have gained popularity because they produce results in a shorted span of time. Rooting is an essential step in the propagation of guava through cuttings. Nowadays, the most popular technique for growing cuttings in a misty environment is the use of plant growth regulators, especially auxins such as Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) that promote root growth. The present study was undertaken to assess the influence of plant growth regulators on the rooting of diverse stem cuttings from guava cv. Arka Kiran under mist chamber conditions at the Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu. Two plant growth regulators, IBA and NAA were employed at various concentrations. These regulators were applied to single-node cuttings with one leaf, double node cuttings with two leaves, and herbaceous cuttings. The highest rooting percentage (55.10%), the duration for bud sprouting (21.13 days), and number of leaves (4.83) were observed in single-node cuttings treated with IBA at a concentration of 3000 ppm concentration. Single-node cuttings treated with IBA at 3000 ppm exhibited superior rooting and sprouting performance compared to Naphthalene Acetic Acid (NAA). Among the three stem cuttings, single-node cuttings with one leaf proved to be the most effective for rapid multiplication.

Performance of Guava Stem Cutting – A Review of Successful Though Non-commercial Propagation Method

“…However, in some species, the leaves are removed to reduce the loss of water due to transpiration (Sharma, 2012). Vanitha et al (2023) comparing cuttings namely single-node cutting with one leaf, double node cuttings with two leaves and herbaceous cuttings on rooting induction and found significant difference rooting success. While, Santoro et al (2010) studied the influence of leaf in the guava rooting and revealed that cutting with a pair of leaves had been superior to the parameters of the fresh and dry root mass while cutting without leaves showed no root formation.This result shows the essentiality of leaves presence for rooting.…”

“…In such circumstances, use of FYM and properly decomposed compost as growing media resolve the issue. Vanitha et al (2023) used growth media composed of sand, soil and well decomposed FYM in a 2:1:1 ratio for successful rooting induction.…”

“…Thus, it is obvious that in spite of highest area and production, country is far behind in terms of productivity (Anonymous, 2018). Vegetative propagation of guava is now performed by inarching, air layering, wedge grafting and budding with varying degree of success (Pereira et al, 2017, Kumar et al, 2022, Vanitha et al, 2023. However, expense of maintaining plant, long duration for producing sellable plants and complex stock-scion relationship are making layering, grafting and budding less suitable method for rapid clonal propagation.…”

“…Joshi, 2024 cuttings with several manipulative treatments viz., wounding, hormonal treatment, growing environment (Siva Prakash et al, 2018, Costa et al, 2019, El-Sharony et al, 2020, Sarjiyah et al, 2021, Vanitha et al, 2023, Yogesh et al, 2023. Although, propagation of guava stem cutting was not so popular method in old days because of its difficult to root nature (Kumar et al, 2022).…”

Does the Physiological Age of Stock Plant Material Affect the Uptake of Indole-3-Butyric Acid (IBA) in Leafy Cuttings of Prunus subhirtella ‘Autumnalis’?

“…The formation of ARs is of great practical importance in horticulture, but many important plant species, especially those belonging to woody species, are difficult to root [9]. Since auxins have been shown to be effective in AR formation, exogenous application of auxin is used to promote rooting of cuttings in several plant species [10][11][12][13]. The most commonly used exogenous auxin is indole-3-butyric acid (IBA).…”