Impact of Salinomycin on human cholangiocarcinoma: induction of apoptosis and impairment of tumor cell proliferation in vitro

Abstract

Background: Cholangiocarcinoma (CC) is a primary liver cancer with increasing incidence worldwide. Despite all efforts made in past years, prognosis remains to be poor. At least in part, this might be explained by a pronounced resistance of CC cells to undergo apoptosis. Thus, new therapeutic strategies are imperatively required. In this study we investigated the effect of Salinomycin, a polyether ionophore antibiotic, on CC cells as an appropriate agent to treat CC. Salinomycin was quite recently identified to induce apoptosis in cancer stem cells and to overcome apoptosis-resistance in several leukemia-cells and other cancer cell lines of different origin. Methods: To delineate the effects of Salinomycin on CC, we established an in vitro cell culture model using three different human CC cell lines. After treatment apoptosis as well as migration and proliferation behavior was assessed and additional cell cycle analyses were performed by flowcytometry.

Geno- and cytotoxicity of salinomycin in human nasal mucosa and peripheral blood lymphocytes

“…Another study showed an inhibition of autophagic activity by salinomycin in hepatocellular carcinoma (Klose et al, 2014). Additionally, salinomycin was introduced as a potent drug to overcome apoptosis resistance in several types of cancer (Lieke et al, 2012). Due to these abilities there is a growing hope of using salinomycin as an anticancer drug.…”